LOCUS       sequence-418225-        2071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_MCD15
FEATURES             Location/Qualifiers
     source          1..2071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(186..280)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(301..960)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     875..894
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1122..1140
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1144..1187)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1234..1291
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1315..1332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1486..2071,1..3))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1566..1585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
       61 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
      181 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      241 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
      301 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      361 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      421 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      481 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgacacga aaaacatatt
      541 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      601 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      661 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      721 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      781 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      841 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      901 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      961 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1021 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
     1081 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1141 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttcggt ctcagtactc
     1201 ttaatcatgc gggggagtct ttcataagag acctccggca aaaaagggca aggtgtcacc
     1261 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     1321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
     1501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1921 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1981 tggtagctct tgatccggca aacaaaccac cgctaccagc ggtggttttt ttgtttgcaa
     2041 gcagcagatt acgcgcagaa aaaaaggatc t
//
