LOCUS       sequence-418232-        2068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 CDS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-C_dummy-CDS
FEATURES             Location/Qualifiers
     source          1..2068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(188..282)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(303..962)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     877..896
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1124..1142
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1146..1189)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1233..1290
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1314..1331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1485..2068,1..5))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1565..1584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
       61 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      121 aaaaattaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga
      181 ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      241 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      301 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      361 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      421 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      481 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgacac gaaaaacata
      541 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
      601 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
      661 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
      721 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      781 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      841 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      901 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      961 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1021 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
     1081 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1141 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttcg acttggtctc
     1201 gcataggtag tgataatcga tgagaccaag tctccggcaa aaaagggcaa ggtgtcacca
     1261 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     1321 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1381 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     1501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1921 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1981 ggtagctctt gatccggcaa acaaaccacc gctaccagcg gtggtttttt tgtttgcaag
     2041 cagcagatta cgcgcagaaa aaaaggat
//
