LOCUS       sequence-418234-        2770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 CDS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-C_mTagBFP2
FEATURES             Location/Qualifiers
     source          1..2770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(177..271)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(292..951)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     866..885
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1113..1131
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1135..1178)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             1194..1904
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      1924..1981
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2005..2022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2176..2764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2256..2275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
       61 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaattaagt
      121 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
      181 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
      241 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
      301 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      361 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      421 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      481 atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat tctcaataaa
      541 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      601 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
      661 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      721 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      781 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      841 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      901 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      961 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     1021 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
     1081 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
     1141 gataaaaaaa atccttagct ttcgctaagg atgatttcga cttggtctcg catatggtgt
     1201 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtatatg gagggcaccg
     1261 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     1321 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     1381 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     1441 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     1501 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     1561 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     1621 gctgggaggc cttcaccgag actctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     1681 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     1741 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     1801 tggaaagaat caaggaggcc aacaacgaaa cctacgtcga gcagcacgag gtggcagtgg
     1861 ccagatactg cgacctccct agcaaactgg ggcacaagct taattaatcg atgagaccaa
     1921 gtctccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     1981 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2161 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     2221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2581 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2701 cgctaccagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2761 tcaagaagat
//
