LOCUS       sequence-418240-        2137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 terminator part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-T_ECK120029600
FEATURES             Location/Qualifiers
     source          1..2137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(177..271)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(292..951)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     866..885
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1113..1131
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1135..1178)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1291..1348
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1372..1389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1543..2131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1623..1642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
       61 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      121 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
      181 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
      241 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
      301 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      361 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      421 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      481 atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat tctcaataaa
      541 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      601 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
      661 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      721 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      781 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      841 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      901 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      961 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     1021 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
     1081 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
     1141 gataaaaaaa atccttagct ttcgctaagg atgatttcgg tctcatcgat tcagccaaaa
     1201 aacttaagac cgccggtctt gtccactacc ttgcagtaat gcggtggaca ggatcggcgg
     1261 ttttcttttc tcttctcaat gttagagacc tccggcaaaa aagggcaagg tgtcaccacc
     1321 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
     1381 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1501 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
     1561 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1621 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1681 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1741 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1801 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1861 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1921 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1981 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2041 tagctcttga tccggcaaac aaaccaccgc taccagcggt ggtttttttg tttgcaagca
     2101 gcagattacg cgcagaaaaa aaggatctca agaagat
//
