LOCUS       sequence-418252-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of anti-GluN3A/NR3A glutamate receptor 
            (Rat) IgG2a R-mAb. Derived from hybridoma N416/40..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-GluN3A/NR3A glutamate receptor [N416/40R]
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2393..2696
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2697..2900
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4160..4855
                     /codon_start=1
                     /product="CH2 and CH3 domains of the mouse IgG2a heavy 
                     chain plus the hinge region"
                     /label=Fc tag
                     /translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
                     CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
                     KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
                     VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
                     TKSFSRTPGK"
     polyA_signal    4906..4961
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4960..4979)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7197..7215)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7283..7387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7388..7456,7457..8248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7606..7625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8419..9007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8908..8927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9161..9178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9295..9316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9331..9336,9337..9354,9355..9361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9369..9385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9374..9396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9393..9409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9393..9409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcagatgttt tgatgaccca aactccactc tccctgcctg
     1501 tcagtcttgg agatcaagcc tccatctctt gcagatctag tcagagcatt gtacatagta
     1561 atggaaacac ctatttagaa tggtacctgc agaaaccagg ccagtctcca aagctcctga
     1621 tctacaaagt ttccaaccga ttttctgggg tcccagacag gttcagtggc agtggatcag
     1681 ggacagattt cacactcaag atcagcagag tggaggctga ggatctggga gtttattact
     1741 gctttcaagg ttcacatgtt cctccgacgt tcggtggagg caccaagctg gaaatcaaac
     1801 gggctgatgc tgcaccaact gtatccatct tcccaccatc cagtgagcag ttaacatctg
     1861 gaggtgcctc agtcgtgtgc ttcttgaaca acttctaccc caaagacatc aatgtcaagt
     1921 ggaagattga tggcagtgaa cgacaaaatg gcgtcctgaa cagttggact gatcaggaca
     1981 gcaaagacag cacctacagc atgagcagca ccctcacgtt gaccaaggac gagtatgaac
     2041 gacataacag ctatacctgt gaggccactc acaagacatc aacttcaccc attgtcaaga
     2101 gcttcaacag gaatgagtgt tagagacaaa ggtcctgaga cgccaccacc agctccccag
     2161 ctccatccta tcttcccttc taaggtcttg gaggcttccc cacgagcgac ctaccactgt
     2221 tgcggtgctc caaacctcct ccccacctcc ttctcctcct cctccctttc cttggctttt
     2281 atcatgctaa tatttgcaga aaatattcaa taaagtgagt ctttgcactt gatagttatt
     2341 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     2401 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2461 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2521 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
     2581 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2641 tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     2701 tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     2761 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2821 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
     2881 aggtctatat aagcagagct cgtttagtga accgtcagat cctcactctc ttccgcatcg
     2941 ctgtctgcga gggccagctg ttgggctcgc ggttgaggac aaactcttcg cggtctttcc
     3001 agtactctcg gatcggaaac ccgtcggcct ccgaacggta ctccgccacc gagggacctg
     3061 agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag
     3121 tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg
     3181 gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgag
     3241 gtgaggtgtg gcaggcttga gatccagctg ttggggtgag tactccctct caaaagcggg
     3301 cattacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcaccta
     3361 gcccgatctg gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg
     3421 tgtccactcc caggccacca tgaagctgcc tgttctgcta gtggtgctgc tattgttcac
     3481 gagtccagcc tcaagcagtg aagtgatgct ggtggagtct gggggaggct tagtgaagcc
     3541 tggagggtcc ctgaaactct cctgtgcagc ctctggattc actttcagta gctatgccat
     3601 gtcttgggtt cgccagactc cggagaagag gctggagtgg gtcgcaacca ttagtagtgg
     3661 tggtagttac acctactatc cagacagtgt gaaggggcga ttcaccatct ccagagacaa
     3721 tgccaagaac accctgtacc tgcaaatgag cagtctgagg tctgaggaca cggccatgta
     3781 ttactgtgca agacaaggct actatggtaa ctacgcctat gctatggact actggggtca
     3841 aggaacctca gtcaccgtct cctcagcgcg cccaacagcc ccatcggtct atccactggc
     3901 ccctgtgtgt ggagatacaa ctggctcctc ggtgactcta ggatgcctgg tcaagggtta
     3961 tttccctgag ccagtgacct tgacctggaa ctctggatcc ctgtccagtg gtgtgcacac
     4021 cttcccagct gtcctgcagt ctgacctcta caccctcagc agctcagtga ctgtaacctc
     4081 gagcacctgg cccagccagt ccatcacctg caatgtggcc cacccggcaa gcagcaccaa
     4141 ggtggacaag aaaattgagc ccagagggcc cacaatcaag ccctgtcctc catgcaaatg
     4201 cccagcacct aacctcttgg gtggaccatc cgtcttcatc ttccctccaa agatcaagga
     4261 tgtactcatg atctccctga gccccatagt cacatgtgtg gtggtggatg tgagcgagga
     4321 tgacccagat gtccagatca gctggtttgt gaacaacgtg gaagtacaca cagctcagac
     4381 acaaacccat agagaggatt acaacagtac tctccgggtg gtcagtgccc tccccatcca
     4441 gcaccaggac tggatgagtg gcaaggagtt caaatgcaag gtcaacaaca aagacctccc
     4501 agcgcccatc gagagaacca tctcaaaacc caaagggtca gtaagagctc cacaggtata
     4561 tgtcttgcct ccaccagaag aagagatgac taagaaacag gtcactctga cctgcatggt
     4621 cacagacttc atgcctgaag acatttacgt ggagtggacc aacaacggga aaacagagct
     4681 aaactacaag aacactgaac cagtcctgga ctctgatggt tcttacttca tgtacagcaa
     4741 gctgagagtg gaaaagaaga actgggtgga aagaaatagc tactcctgtt cagtggtcca
     4801 cgagggtctg cacaatcacc acacgactaa gagcttctcc cggactccgg gtaaatgatc
     4861 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     4921 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4981 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     5041 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     5101 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     5161 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     5221 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     5281 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     5341 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     5401 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     5461 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     5521 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     5581 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     5641 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     5701 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     5761 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     5821 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     5881 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     5941 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     6001 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     6061 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     6121 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     6181 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     6241 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     6301 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     6361 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     6421 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     6481 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     6541 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     6601 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     6661 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     6721 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     6781 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     6841 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     6901 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     6961 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     7021 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     7081 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     7141 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     7201 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7261 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7321 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7381 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7441 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7501 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7561 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7621 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7681 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7741 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7801 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7861 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7921 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7981 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8041 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8101 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8161 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8221 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8521 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9181 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9241 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9301 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9361 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgac
//
