LOCUS       sequence-418256-        2081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 terminator part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-T_his-min-S
FEATURES             Location/Qualifiers
     source          1..2081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(180..274)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(295..954)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     869..888
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1116..1134
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1138..1181)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1238..1295
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1319..1336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1490..2078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1570..1589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
       61 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga
      181 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
      241 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
      301 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      361 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      421 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      481 taaatcaaaa ctggtgaaac tcacccaggg attggctgac acgaaaaaca tattctcaat
      541 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
      601 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
      661 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      721 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      781 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      841 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      901 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      961 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1021 attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct
     1081 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt
     1141 tcagataaaa aaaatcctta gctttcgcta aggatgattt cggtctcatc gagcccccgg
     1201 aagatcattc cgggggcttt tttatttgtt agagacctcc ggcaaaaaag ggcaaggtgt
     1261 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct
     1321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
     1501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1921 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctac cagcggtggt ttttttgttt
     2041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga a
//
