LOCUS       sequence-418263-        9413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of anti-TrpC6 (Human) IgG2a R-mAb. 
            Derived from hybridoma N490/24..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-TrpC6 [N490/24R]
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2394..2697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2698..2901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4164..4859
                     /codon_start=1
                     /product="CH2 and CH3 domains of the mouse IgG2a heavy 
                     chain plus the hinge region"
                     /label=Fc tag
                     /translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
                     CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
                     KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
                     VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
                     TKSFSRTPGK"
     polyA_signal    4910..4965
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4964..4983)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7201..7219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7287..7391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7392..7460,7461..8252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7610..7629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8423..9011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8912..8931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9165..9182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9299..9320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9335..9340,9341..9358,9359..9365)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9373..9389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9378..9400
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9397..9413
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9397..9413
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcagatgttt tgatgaccca aactccactc tccctgcctg
     1501 tcagtcttgg agatcaagcc tccatctctt gcagatctag tcagagcatt gtacatagta
     1561 atggaaacac ctatttagaa tggtacctgc agaaaccagg ccagtctcca aagctcctga
     1621 tctacaaagt ttccaaccga ttttctgggg tcccagacag gttcagtggc agtggatcag
     1681 ggacagattt cacactcaag atcagcagag tggaggctga ggatctggga gtttattact
     1741 gctttcaagg ttcacatgtt cctctcacgt tcggctcggg gacaaagttg gaaataaaac
     1801 gggctgatgc tgcaccaact gtatccatct tcccaccatc cagtgagcag nttaacatct
     1861 ggaggtgcct cagtcgtgtg cttcttgaac aacttctacc ccaaagacat caatgtcaag
     1921 tggaagattg atggcagtga acgacaaaat ggcgtcctga acagttggac tgatcaggac
     1981 agcaaagaca gcacctacag catgagcagc accctcacgt tgaccaagga cgagtatgaa
     2041 cgacataaca gctatacctg tgaggccact cacaagacat caacttcacc cattgtcaag
     2101 agcttcaaca ggaatgagtg ttagagacaa aggtcctgag acgccaccac cagctcccca
     2161 gctccatcct atcttccctt ctaaggtctt ggaggcttcc ccacgagcga cctaccactg
     2221 ttgcggtgct ccaaacctcc tccccacctc cttctcctcc tcctcccttt ccttggcttt
     2281 tatcatgcta atatttgcag aaaatattca ataaagtgag tctttgcact tgatagttat
     2341 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2401 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2461 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2521 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     2581 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2641 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2701 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2761 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2821 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
     2881 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcctcactct cttccgcatc
     2941 gctgtctgcg agggccagct gttgggctcg cggttgagga caaactcttc gcggtctttc
     3001 cagtactctc ggatcggaaa cccgtcggcc tccgaacggt actccgccac cgagggacct
     3061 gagcgagtcc gcatcgaccg gatcggaaaa cctctcgaga aaggcgtcta accagtcaca
     3121 gtcgcaaggt aggctgagca ccgtggcggg cggcagcggg tggcggtcgg ggttgtttct
     3181 ggcggaggtg ctgctgatga tgtaattaaa gtaggcggtc ttgagacggc ggatggtcga
     3241 ggtgaggtgt ggcaggcttg agatccagct gttggggtga gtactccctc tcaaaagcgg
     3301 gcattacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
     3361 agcccgatct ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag
     3421 gtgtccactc ccaggccacc atgaagctgc ctgttctgct agtggtgctg ctattgttca
     3481 cgagtccagc ctcaagcagt caagttactc taaaagagtc tggccctggg atattgaagc
     3541 cctcacagac cctcagtctg acctgttctt tctctgggtt ttcactgagc acttctggta
     3601 tgggtgtagg ctggattcgt cagccttcag ggaagggtct ggagtggctg gcacacattt
     3661 ggtgggatga ttataagtac tataacccat ccctgaagag ccagctcaca atctccaagg
     3721 atacctccag aaaccaggta ttcctcaaga tcaccagtgt ggacactgca gatactgcca
     3781 cttactactg tgctcgaaga gatggtaact acgtagggtc ctatgctatg gactactggg
     3841 gtcaaggaac ctcagtcacc gtctcctcag cgcgcccaac agccccatcg gtctatccac
     3901 tggcccctgt gtgtggagat acaactggct cctcggtgac tctaggatgc ctggtcaagg
     3961 gttatttccc tgagccagtg accttgacct ggaactctgg atccctgtcc agtggtgtgc
     4021 acaccttccc agctgtcctg cagtctgacc tctacaccct cagcagctca gtgactgtaa
     4081 cctcgagcac ctggcccagc cagtccatca cctgcaatgt ggcccacccg gcaagcagca
     4141 ccaaggtgga caagaaaatt gagcccagag ggcccacaat caagccctgt cctccatgca
     4201 aatgcccagc acctaacctc ttgggtggac catccgtctt catcttccct ccaaagatca
     4261 aggatgtact catgatctcc ctgagcccca tagtcacatg tgtggtggtg gatgtgagcg
     4321 aggatgaccc agatgtccag atcagctggt ttgtgaacaa cgtggaagta cacacagctc
     4381 agacacaaac ccatagagag gattacaaca gtactctccg ggtggtcagt gccctcccca
     4441 tccagcacca ggactggatg agtggcaagg agttcaaatg caaggtcaac aacaaagacc
     4501 tcccagcgcc catcgagaga accatctcaa aacccaaagg gtcagtaaga gctccacagg
     4561 tatatgtctt gcctccacca gaagaagaga tgactaagaa acaggtcact ctgacctgca
     4621 tggtcacaga cttcatgcct gaagacattt acgtggagtg gaccaacaac gggaaaacag
     4681 agctaaacta caagaacact gaaccagtcc tggactctga tggttcttac ttcatgtaca
     4741 gcaagctgag agtggaaaag aagaactggg tggaaagaaa tagctactcc tgttcagtgg
     4801 tccacgaggg tctgcacaat caccacacga ctaagagctt ctcccggact ccgggtaaat
     4861 gatctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa
     4921 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     4981 gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc
     5041 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
     5101 gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg
     5161 gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt
     5221 gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg
     5281 caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg
     5341 acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg
     5401 ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag
     5461 gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc
     5521 agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag
     5581 ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt
     5641 tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg
     5701 gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc
     5761 cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct
     5821 cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg
     5881 gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt
     5941 aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt
     6001 aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc
     6061 aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg
     6121 ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca
     6181 ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa
     6241 caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc
     6301 acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa
     6361 ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga
     6421 atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga
     6481 caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg
     6541 aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc
     6601 tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     6661 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     6721 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6781 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt
     6841 agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga
     6901 tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     6961 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     7021 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     7081 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     7141 tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg
     7201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7621 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7921 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9181 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9241 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9301 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9361 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gac
//
