LOCUS       sequence-418264-       10096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Type 0 tag parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pMetBP-Tag_cRed_BsaI
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(158..252)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(273..932)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     847..866
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1094..1112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1116..1159)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      7270..7279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      9155..9202
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      9231..9288
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(9312..9329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9483..10071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9563..9582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
       61 aaaggatctt cacctagatc cttttaaatt aaaaattaag ttttaaatca atctaaagta
      121 tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc
      181 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
      241 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
      301 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      361 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      421 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      481 acccagggat tggctgacac gaaaaacata ttctcaataa accctttagg gaaataggcc
      541 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      601 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
      661 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
      721 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
      781 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
      841 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
      901 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
      961 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1021 cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1081 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
     1141 tttcgctaag gatgatttcg acttggtctc ataaatgaga ccgcagcggt ctgccaatcc
     1201 gacggaggtt tatgaatttt ccaccttttc cacaagctca actagtatta acgatgtgga
     1261 tttagcaaaa aaaacctgta accctaaatg taaaataacg ggtaagcctg ccaaccatgt
     1321 tatggcagat taagcgtctt tttgaagggc accgcatctt tcgcgttgcc gtaaatgtat
     1381 ccgtttagca ataaggaaat tcttaatgaa taatccgtcg ttactcaatc atgcggtcga
     1441 aacgatggca gttggctcga aaagttttgc gacagcctca aagttatttg atgcaaaaac
     1501 ccggcgcagc gtactgatgc tctacgcctg gtgccgccat tgtgacgatg ttattgacga
     1561 tcagacgctg ggctttcagg cccggcagcc tgccttacaa acgcccgaac aacgtctgat
     1621 gcaacttgag atgaaaacgc gccaggccta tgcaggatcg cagatgcacg aaccggcgtt
     1681 tgcggctttt cgggaagtgg ctatggctca tgatatcgcc ccggcttacg cgtttgatca
     1741 tctggaaggc ttcgccatgg atgtacgcga agcgcaatac agccaactgg atgatacgct
     1801 gcgctattgc tatcacgttg caggcgttgt cggcttgatg atggcgcaaa tcatgggcgt
     1861 gcgggataac gccacgctgg accgcgcctg tgaccttggg ctggcatttc agttgaccaa
     1921 tattgctcgc gatattgtgg acgatgcgca tgcgggccgc tgttatctgc cggcaagctg
     1981 gctggagcat gaaggtctga acaaagagaa ttatgcggca cctgaaaacc gtcaggcgct
     2041 gagccgtatc gcccgtcgtt tggtgcagga agcagaacct tactatttgt ctgccacagc
     2101 cggcctggca gggttgcccc tgcgttccgc ctgggcaatc gctacggcga agcaggttta
     2161 ccggaaaata ggtgtcaaag ttgaacaggc cggtcagcaa gcctgggatc agcggcagtc
     2221 aacgaccacg cccgaaaaat taacgctgct gctggccgcc tctggtcagg cccttacttc
     2281 ccggatgcgg gctcatcctc cccgccctgc gcatctctgg cagcgcccgc tctaggctta
     2341 ctataaggat gtttgcaatg aaaccaacta cggtaattgg tgcaggcttc ggtggcctgg
     2401 cactggcaat tcgtctacaa gctgcgggga tccccgtctt actgcttgaa caacgtgata
     2461 aacccggcgg tcgggcttat gtctacgagg atcaggggtt tacctttgat gcaggcccga
     2521 cggttatcac cgatcccagt gccattgaag aactgtttgc actggcagga aaacagttaa
     2581 aagagtatgt cgaactgctg ccggttacgc cgttttaccg cctgtgttgg gagtcaggga
     2641 aggtctttaa ttacgataac gatcaaaccc ggctcgaagc gcagattcag cagtttaatc
     2701 cccgcgatgt cgaaggttat cgtcagtttc tggactattc acgcgcggtg tttaaagaag
     2761 gctatctaaa gctcggtact gtcccttttt tatcgttcag agacatgctt cgcgccgcac
     2821 ctcaactggc gaaactgcag gcatggagaa gcgtttacag taaggttgcc agttacatcg
     2881 aagatgaaca tctgcgccag gcgttttctt tccactcgct gttggtgggc ggcaatccct
     2941 tcgccacctc atccatttat acgttgatac acgcgctgga gcgtgagtgg ggcgtctggt
     3001 ttccgcgtgg cggcaccggc gcattagttc aggggatgat aaagctgttt caggatctgg
     3061 gtggcgaagt cgtgttaaac gccagagtca gccatatgga aacgacagga aacaagattg
     3121 aagccgtgca tttagaggac ggtcgcaggt tcctgacgca agccgtcgcg tcaaatgcag
     3181 atgtggttca tacctatcgc gacctgttaa gccagcaccc tgccgcggtt aagcagtcca
     3241 acaaactgca gactaagcgc atgagtaact ctctgtttgt gctctatttt ggtttgaatc
     3301 accatcatga tcagctcgcg catcacacgg tttgtttcgg cccgcgttac cgcgagctga
     3361 ttgacgaaat ttttaatcat gatggcctcg cagaggactt ctcactttat ctgcacgcgc
     3421 cctgtgtcac ggattcgtca ctggcgcctg aaggttgcgg cagttactat gtgttggcgc
     3481 cggtgccgca tttaggcacc gcgaacctcg actggacggt tgaggggcca aaactacgcg
     3541 accgtatttt tgcgtacctt gagcagcatt acatgcctgg cttacggagt cagctggtca
     3601 cgcaccggat gtttacgccg tttgattttc gcgaccagct taatgcctat catggctcag
     3661 ccttttctgt ggagcccgtt cttacccaga gcgcctggtt tcggccgcat aaccgcgata
     3721 aaaccattac taatctctac ctggtcggcg caggcacgca tcccggcgca ggcattcctg
     3781 gcgtcatcgg ctcggcaaaa gcgacagcag gtttgatgct ggaggatctg atatgagctt
     3841 ttacccagga gacagagaat gcaaccgcat tatgatctga ttctcgtggg ggctggactc
     3901 gcgaatggcc ttatcgccct gcgacttcag cagcagcaac ctgatatgcg tattttgctt
     3961 atcgacgccg caccccaggc gggcgggaat catacgtggt catttcacca cgatgatttg
     4021 actgagagcc aacatcgttg gatagctccg ctggtggttc atcactggcc cgactatcag
     4081 gtacgctttc ccacacgccg tcgtaagctg aacagcggct acttttgtat tacttctcag
     4141 cgtttcgctg aggttttaca gcgacagttt ggcccgcact tgtggatgga taccgcggtc
     4201 gcagaggtta atgcggaatc tgttcggttg aaaaagggtc aggttatcgg tgcccgcgcg
     4261 gtgattgacg ggcggggtta tgcggcaaat tcagcactga gcgtgggctt ccaggcgttt
     4321 attggccagg aatggcgatt gagtcacccg catggtttat catctccgat tatcatggat
     4381 gccacggtcg atcagcaaaa tggttatcgc ttcgtgtaca gcctgccgct ctcgccgacc
     4441 agattgttaa ttgaagatac gcactatatt gataatgcga cattagatcc tgaatgcgcg
     4501 cggcaaaata tttgcgacta tgccgcgcaa cagggttggc agcttcagac actgctgcga
     4561 gaagaacagg gcgccttacc cattactctg tcgggcaatg ccgacgcatt ctggcagcag
     4621 cgccccctgg cctgtagtgg attacgtgcc ggtctgttcc atcctaccac cggctattca
     4681 ctgccgctgg cggttgccgt ggccgaccgc ctgagtgcac ttgatgtctt tacgtcggcc
     4741 tcaattcacc atgccattac gcattttgcc cgcgagcgct ggcagcagca gggctttttc
     4801 cgcatgctga atcgcatgct gtttttagcc ggacccgccg attcacgctg gcgggttatg
     4861 cagcgttttt atggtttacc tgaagattta attgcccgtt tttatgcggg aaaactcacg
     4921 ctgaccgatc ggctacgtat tctgagcggc aagccgcctg ttccggtatt agcagcattg
     4981 caagccatta tgacgactca tcgttaagct tcagaggagg aattaagtaa tgagttttga
     5041 tattgccaaa tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc
     5101 gaaagagagt ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag
     5161 ccgttccagc gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca
     5221 ctatgtctac aacaccccgt ttgaccaatt gatttgggat gtggggcatc aggcttatcc
     5281 gcataaaatt ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggtct
     5341 gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc
     5401 aacctccatt agtgccggaa ttggtattgc ggttgctgcc gaaaaagaag gcaaaaatcg
     5461 ccgcaccgtc tgtgtcattg gcgatggcgc gattaccgca ggcatggcgt ttgaagcgat
     5521 gaatcacgcg ggcgatatcc gtcctgatat gctggtgatt ctcaacgaca atgaaatgtc
     5581 gatttccgaa aatgtcggcg cgctcaacaa ccatctggca cagctgcttt ccggtaagct
     5641 ttactcttca ctgcgcgaag gcgggaaaaa agttttctct ggcgtgccgc caattaaaga
     5701 gctgctcaaa cgcaccgaag aacatattaa aggcatggta gtgcctggca cgttgtttga
     5761 ggagctgggc tttaactaca tcggcccggt ggacggtcat gatgtgctgg ggcttatcac
     5821 cacgctaaag aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa
     5881 aggtcgtggt tatgaaccgg cagaaaaaga cccgatcact ttccatgccg tgctaaattt
     5941 gatccctcca gcggttgttt gccgaaaagt agcggcggtt tgccgagcta ttcaaaaatc
     6001 tttggcgact ggttgtgcga aacggcagcg aaagacaaca agctgatggc gattactccg
     6061 gcgatgcgtg aaggttccgg catggtcgag ttttcacgta aattcccgga tcgctacttc
     6121 gacgtggcaa ttgccgagca acatgcggtg acctttgctg cgggtctggc gattggtggg
     6181 tacaaaccca ttgtcgcgat ttactccact ttcctgcaac gcgcctatga tcaggtgctg
     6241 catgacgtgg cgattcaaaa gcttccggtc ctgttcgcca tcgaccgcgc gggcattgtt
     6301 ggtgctgacg gtcaaaccca tcagggtgct tttgatctct cttacctgcg ctgcataccg
     6361 gaaatggtca ttatgacccc gagcgatgaa aacgaatgtc gccagatgct ctataccggc
     6421 tatcactata acgatggccc gtcagcggtg cgctacccgc gtggcaacgc ggtcggcgtg
     6481 gaactgacgc cgctggaaaa actaccaatt ggcaaaggca ttgtgaagcg tcgtggcgag
     6541 aaactggcga tccttaactt tggtacgctg atgccagaag cggcgaaagt cgccaaatcg
     6601 ctgaacgcca cgctggtcga tatgcgtttt gtgaaaccgc ttgatgaagc gttaattctg
     6661 gaaatggccg ccagccatga agcgctggtc accgtagaag aaaacgccat tatgggcggc
     6721 gcaggcagcg gcgtgaacga agtgctgatg gcccatcgta aaccagtacc cgtgctgaac
     6781 attggcctgc cggacttctt tattccgcaa ggaactcagg aagaaatgcg cgccgaactc
     6841 ggcctcgatg ccgctggtat ggaagccaaa atcaaggcct ggctggcata agctttgtgt
     6901 gaggagatgt cgaatgagcg cacatgccct gcccaaggca gacctgaccg ccaccagcct
     6961 gatcgtatcg ggcggcatca tcgccgcttg gctggccctg catgtgcatg cgctgtggtt
     7021 tctggacgca gcggcgcatc ccatcctggc gatcgcaaat ttcctggggc tgacctggct
     7081 gtcggtcgga ttgttcatca tcgcgcatga cgcgatgcac gggtcggtgg tgccggggcg
     7141 tccgcgcgcc aatgcggcga tgggccagct tgtcctgtgg ctgtatgccg gattttcgtg
     7201 gcgcaagatg atcgtcaagc acatggccca tcaccgccat gccggaaccg acgacgaccc
     7261 cgatttcgac catggcggcc cggtccgctg gtacgcccgc ttcatcggca cctatttcgg
     7321 ctggcgcgag gggctgcttc tgcccgtcat cgtgacggtc tatgcgctga tccttgggga
     7381 tcgctggatg tacgtggtat tctggccgct gccgtcgatc ctggcgtcga tccagctgtt
     7441 cgtgttcggc acctggctgc cgcaccgccc cggccacgac gcgttcccgg accgccacaa
     7501 tgcgcggtcg tcgcggatca gcgaccccgt gtcgctgctg acctgttttc actttggcgg
     7561 ttatcatcac gaacaccacc tgcacccgac ggtgccgtgg tggcgcctgc ccagcacccg
     7621 caccaagggc gacaccgcat aagcttgagc tgaggagatt ttgaatgcaa acggaacacg
     7681 tcattttatt gaatgcacag ggagttccca cgggtacgct ggaaaagtat gccgcacaca
     7741 cggcagacac ccgcttacat ctcgcgttct ccagttggct gtttaatgcc aaaggacaat
     7801 tattagttac ccgccgcgca ctgagcaaaa aagcatggcc tggcgtgtgg actaactcgg
     7861 tttgtgggca cccacaactg ggagaaagca acgaggacgc agtgatccgc cgttgccgtt
     7921 atgagcttgg cgtggaaatt acgcctcctg aatctatcta tcctgacttt cgctaccgcg
     7981 ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc ggtatttgcc gcacgcacca
     8041 ctagtgcgtt acagatcaat gatgatgaag tgatggatta tcaatggtgt gatttagcag
     8101 atgtattaca cggtattgat gcaacgccgt gggcgttcag tccgtggatg gtgatgcagg
     8161 cgacaaatcg cgaagccaga aaacgattat ctgcatttac ccagcttaaa taagctttgc
     8221 ctcaggattt ttgaaatgac ggtctgcgca aaaaaacacg ttcatctcac tcgcgatgct
     8281 gcggagcagt tactggctga tattgatcga cgccttgatc agttattgcc cgtggaggga
     8341 gaacgggatg ttgtgggtgc cgcgatgcgt gaaggtgcgc tggcaccggg aaaacgtatt
     8401 cgccccatgt tgctgttgct gaccgcccgc gatctgggtt gcgctttcag ccatgacgga
     8461 ttactggatt tggcctgtgc ggtggaaatg gtccacgcgg cttcgctgat ccttgacgat
     8521 atgccctgca tggacgatgc gaagctgcgg cgcggacgcc ctaccattca ttctcattac
     8581 ggagagcatg tggcaatact ggcggcggtt gccttgctga gtaaagcctt tggcgtaatt
     8641 gccgatgcag atggcctcac gccgctggca aaaaatcggg cggtttctga actgtcaaac
     8701 gccatcggca tgcaaggatt ggttcagggt cagttcaagg atctgtctga aggggataag
     8761 ccgcgcagcg ctgaagctat tttgatgacg aatcacttta aaaccagcac gctgttttgt
     8821 gcctcgatgc agatggcctc gattgttgcg aatgcctcca gcgaagcgcg tgattgcctg
     8881 catcgttttt cacttgatct tggtcaggca tttcaactgc tggacgattt gaccgatggc
     8941 atgaccgaca ccggtaagga tagcaatcag gacgccggta aatcgacgct ggtcaatctg
     9001 ttaggcccga gggcggttga agaacgtctg agacaacatc ttcagcttgc cagtgagcat
     9061 ctctctgcgg cctgccaaca cgggcacgcc actcaacatt ttattcaggc ctggtttgac
     9121 aaaaaactcg ctgccgtcag ttaagcttgc aagactagca taaccccttg gggcctctaa
     9181 acgggtcttg aggggttttt tggtgacggt ctcacatacg agaccaagtc tccggcaaaa
     9241 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
     9301 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9361 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9481 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9841 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9901 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9961 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc taccagcggt
    10021 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10081 ttgatctttt ctacgg
//
