LOCUS       sequence-418265-       10096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Type 0 CDS parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pMetBP-C_cRed_BsaI
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(164..258)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(279..938)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     853..872
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1100..1118
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1122..1165)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      7276..7285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      9161..9208
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      9237..9294
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(9318..9335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9489..10077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9569..9588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
       61 tatcaaaaag gatcttcacc tagatccttt taaattaaaa attaagtttt aaatcaatct
      121 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
      181 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      241 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
      301 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      361 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      421 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
      481 aaactcaccc agggattggc tgacacgaaa aacatattct caataaaccc tttagggaaa
      541 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
      601 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
      661 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
      721 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
      781 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
      841 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
      901 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
      961 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1021 gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat
     1081 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     1141 cttagctttc gctaaggatg atttcgactt ggtctcgcat atgagaccgc agcggtctgc
     1201 caatccgacg gaggtttatg aattttccac cttttccaca agctcaacta gtattaacga
     1261 tgtggattta gcaaaaaaaa cctgtaaccc taaatgtaaa ataacgggta agcctgccaa
     1321 ccatgttatg gcagattaag cgtctttttg aagggcaccg catctttcgc gttgccgtaa
     1381 atgtatccgt ttagcaataa ggaaattctt aatgaataat ccgtcgttac tcaatcatgc
     1441 ggtcgaaacg atggcagttg gctcgaaaag ttttgcgaca gcctcaaagt tatttgatgc
     1501 aaaaacccgg cgcagcgtac tgatgctcta cgcctggtgc cgccattgtg acgatgttat
     1561 tgacgatcag acgctgggct ttcaggcccg gcagcctgcc ttacaaacgc ccgaacaacg
     1621 tctgatgcaa cttgagatga aaacgcgcca ggcctatgca ggatcgcaga tgcacgaacc
     1681 ggcgtttgcg gcttttcggg aagtggctat ggctcatgat atcgccccgg cttacgcgtt
     1741 tgatcatctg gaaggcttcg ccatggatgt acgcgaagcg caatacagcc aactggatga
     1801 tacgctgcgc tattgctatc acgttgcagg cgttgtcggc ttgatgatgg cgcaaatcat
     1861 gggcgtgcgg gataacgcca cgctggaccg cgcctgtgac cttgggctgg catttcagtt
     1921 gaccaatatt gctcgcgata ttgtggacga tgcgcatgcg ggccgctgtt atctgccggc
     1981 aagctggctg gagcatgaag gtctgaacaa agagaattat gcggcacctg aaaaccgtca
     2041 ggcgctgagc cgtatcgccc gtcgtttggt gcaggaagca gaaccttact atttgtctgc
     2101 cacagccggc ctggcagggt tgcccctgcg ttccgcctgg gcaatcgcta cggcgaagca
     2161 ggtttaccgg aaaataggtg tcaaagttga acaggccggt cagcaagcct gggatcagcg
     2221 gcagtcaacg accacgcccg aaaaattaac gctgctgctg gccgcctctg gtcaggccct
     2281 tacttcccgg atgcgggctc atcctccccg ccctgcgcat ctctggcagc gcccgctcta
     2341 ggcttactat aaggatgttt gcaatgaaac caactacggt aattggtgca ggcttcggtg
     2401 gcctggcact ggcaattcgt ctacaagctg cggggatccc cgtcttactg cttgaacaac
     2461 gtgataaacc cggcggtcgg gcttatgtct acgaggatca ggggtttacc tttgatgcag
     2521 gcccgacggt tatcaccgat cccagtgcca ttgaagaact gtttgcactg gcaggaaaac
     2581 agttaaaaga gtatgtcgaa ctgctgccgg ttacgccgtt ttaccgcctg tgttgggagt
     2641 cagggaaggt ctttaattac gataacgatc aaacccggct cgaagcgcag attcagcagt
     2701 ttaatccccg cgatgtcgaa ggttatcgtc agtttctgga ctattcacgc gcggtgttta
     2761 aagaaggcta tctaaagctc ggtactgtcc cttttttatc gttcagagac atgcttcgcg
     2821 ccgcacctca actggcgaaa ctgcaggcat ggagaagcgt ttacagtaag gttgccagtt
     2881 acatcgaaga tgaacatctg cgccaggcgt tttctttcca ctcgctgttg gtgggcggca
     2941 atcccttcgc cacctcatcc atttatacgt tgatacacgc gctggagcgt gagtggggcg
     3001 tctggtttcc gcgtggcggc accggcgcat tagttcaggg gatgataaag ctgtttcagg
     3061 atctgggtgg cgaagtcgtg ttaaacgcca gagtcagcca tatggaaacg acaggaaaca
     3121 agattgaagc cgtgcattta gaggacggtc gcaggttcct gacgcaagcc gtcgcgtcaa
     3181 atgcagatgt ggttcatacc tatcgcgacc tgttaagcca gcaccctgcc gcggttaagc
     3241 agtccaacaa actgcagact aagcgcatga gtaactctct gtttgtgctc tattttggtt
     3301 tgaatcacca tcatgatcag ctcgcgcatc acacggtttg tttcggcccg cgttaccgcg
     3361 agctgattga cgaaattttt aatcatgatg gcctcgcaga ggacttctca ctttatctgc
     3421 acgcgccctg tgtcacggat tcgtcactgg cgcctgaagg ttgcggcagt tactatgtgt
     3481 tggcgccggt gccgcattta ggcaccgcga acctcgactg gacggttgag gggccaaaac
     3541 tacgcgaccg tatttttgcg taccttgagc agcattacat gcctggctta cggagtcagc
     3601 tggtcacgca ccggatgttt acgccgtttg attttcgcga ccagcttaat gcctatcatg
     3661 gctcagcctt ttctgtggag cccgttctta cccagagcgc ctggtttcgg ccgcataacc
     3721 gcgataaaac cattactaat ctctacctgg tcggcgcagg cacgcatccc ggcgcaggca
     3781 ttcctggcgt catcggctcg gcaaaagcga cagcaggttt gatgctggag gatctgatat
     3841 gagcttttac ccaggagaca gagaatgcaa ccgcattatg atctgattct cgtgggggct
     3901 ggactcgcga atggccttat cgccctgcga cttcagcagc agcaacctga tatgcgtatt
     3961 ttgcttatcg acgccgcacc ccaggcgggc gggaatcata cgtggtcatt tcaccacgat
     4021 gatttgactg agagccaaca tcgttggata gctccgctgg tggttcatca ctggcccgac
     4081 tatcaggtac gctttcccac acgccgtcgt aagctgaaca gcggctactt ttgtattact
     4141 tctcagcgtt tcgctgaggt tttacagcga cagtttggcc cgcacttgtg gatggatacc
     4201 gcggtcgcag aggttaatgc ggaatctgtt cggttgaaaa agggtcaggt tatcggtgcc
     4261 cgcgcggtga ttgacgggcg gggttatgcg gcaaattcag cactgagcgt gggcttccag
     4321 gcgtttattg gccaggaatg gcgattgagt cacccgcatg gtttatcatc tccgattatc
     4381 atggatgcca cggtcgatca gcaaaatggt tatcgcttcg tgtacagcct gccgctctcg
     4441 ccgaccagat tgttaattga agatacgcac tatattgata atgcgacatt agatcctgaa
     4501 tgcgcgcggc aaaatatttg cgactatgcc gcgcaacagg gttggcagct tcagacactg
     4561 ctgcgagaag aacagggcgc cttacccatt actctgtcgg gcaatgccga cgcattctgg
     4621 cagcagcgcc ccctggcctg tagtggatta cgtgccggtc tgttccatcc taccaccggc
     4681 tattcactgc cgctggcggt tgccgtggcc gaccgcctga gtgcacttga tgtctttacg
     4741 tcggcctcaa ttcaccatgc cattacgcat tttgcccgcg agcgctggca gcagcagggc
     4801 tttttccgca tgctgaatcg catgctgttt ttagccggac ccgccgattc acgctggcgg
     4861 gttatgcagc gtttttatgg tttacctgaa gatttaattg cccgttttta tgcgggaaaa
     4921 ctcacgctga ccgatcggct acgtattctg agcggcaagc cgcctgttcc ggtattagca
     4981 gcattgcaag ccattatgac gactcatcgt taagcttcag aggaggaatt aagtaatgag
     5041 ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg agttacgact
     5101 gttgccgaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt tactcgacag
     5161 cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac tgaccgtggc
     5221 gctgcactat gtctacaaca ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc
     5281 ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg
     5341 cggtctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca
     5401 ttcatcaacc tccattagtg ccggaattgg tattgcggtt gctgccgaaa aagaaggcaa
     5461 aaatcgccgc accgtctgtg tcattggcga tggcgcgatt accgcaggca tggcgtttga
     5521 agcgatgaat cacgcgggcg atatccgtcc tgatatgctg gtgattctca acgacaatga
     5581 aatgtcgatt tccgaaaatg tcggcgcgct caacaaccat ctggcacagc tgctttccgg
     5641 taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg tgccgccaat
     5701 taaagagctg ctcaaacgca ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt
     5761 gtttgaggag ctgggcttta actacatcgg cccggtggac ggtcatgatg tgctggggct
     5821 tatcaccacg ctaaagaaca tgcgcgacct gaaaggcccg cagttcctgc atatcatgac
     5881 caaaaaaggt cgtggttatg aaccggcaga aaaagacccg atcactttcc atgccgtgct
     5941 aaatttgatc cctccagcgg ttgtttgccg aaaagtagcg gcggtttgcc gagctattca
     6001 aaaatctttg gcgactggtt gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt
     6061 actccggcga tgcgtgaagg ttccggcatg gtcgagtttt cacgtaaatt cccggatcgc
     6121 tacttcgacg tggcaattgc cgagcaacat gcggtgacct ttgctgcggg tctggcgatt
     6181 ggtgggtaca aacccattgt cgcgatttac tccactttcc tgcaacgcgc ctatgatcag
     6241 gtgctgcatg acgtggcgat tcaaaagctt ccggtcctgt tcgccatcga ccgcgcgggc
     6301 attgttggtg ctgacggtca aacccatcag ggtgcttttg atctctctta cctgcgctgc
     6361 ataccggaaa tggtcattat gaccccgagc gatgaaaacg aatgtcgcca gatgctctat
     6421 accggctatc actataacga tggcccgtca gcggtgcgct acccgcgtgg caacgcggtc
     6481 ggcgtggaac tgacgccgct ggaaaaacta ccaattggca aaggcattgt gaagcgtcgt
     6541 ggcgagaaac tggcgatcct taactttggt acgctgatgc cagaagcggc gaaagtcgcc
     6601 aaatcgctga acgccacgct ggtcgatatg cgttttgtga aaccgcttga tgaagcgtta
     6661 attctggaaa tggccgccag ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg
     6721 ggcggcgcag gcagcggcgt gaacgaagtg ctgatggccc atcgtaaacc agtacccgtg
     6781 ctgaacattg gcctgccgga cttctttatt ccgcaaggaa ctcaggaaga aatgcgcgcc
     6841 gaactcggcc tcgatgccgc tggtatggaa gccaaaatca aggcctggct ggcataagct
     6901 ttgtgtgagg agatgtcgaa tgagcgcaca tgccctgccc aaggcagacc tgaccgccac
     6961 cagcctgatc gtatcgggcg gcatcatcgc cgcttggctg gccctgcatg tgcatgcgct
     7021 gtggtttctg gacgcagcgg cgcatcccat cctggcgatc gcaaatttcc tggggctgac
     7081 ctggctgtcg gtcggattgt tcatcatcgc gcatgacgcg atgcacgggt cggtggtgcc
     7141 ggggcgtccg cgcgccaatg cggcgatggg ccagcttgtc ctgtggctgt atgccggatt
     7201 ttcgtggcgc aagatgatcg tcaagcacat ggcccatcac cgccatgccg gaaccgacga
     7261 cgaccccgat ttcgaccatg gcggcccggt ccgctggtac gcccgcttca tcggcaccta
     7321 tttcggctgg cgcgaggggc tgcttctgcc cgtcatcgtg acggtctatg cgctgatcct
     7381 tggggatcgc tggatgtacg tggtattctg gccgctgccg tcgatcctgg cgtcgatcca
     7441 gctgttcgtg ttcggcacct ggctgccgca ccgccccggc cacgacgcgt tcccggaccg
     7501 ccacaatgcg cggtcgtcgc ggatcagcga ccccgtgtcg ctgctgacct gttttcactt
     7561 tggcggttat catcacgaac accacctgca cccgacggtg ccgtggtggc gcctgcccag
     7621 cacccgcacc aagggcgaca ccgcataagc ttgagctgag gagattttga atgcaaacgg
     7681 aacacgtcat tttattgaat gcacagggag ttcccacggg tacgctggaa aagtatgccg
     7741 cacacacggc agacacccgc ttacatctcg cgttctccag ttggctgttt aatgccaaag
     7801 gacaattatt agttacccgc cgcgcactga gcaaaaaagc atggcctggc gtgtggacta
     7861 actcggtttg tgggcaccca caactgggag aaagcaacga ggacgcagtg atccgccgtt
     7921 gccgttatga gcttggcgtg gaaattacgc ctcctgaatc tatctatcct gactttcgct
     7981 accgcgccac cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta tttgccgcac
     8041 gcaccactag tgcgttacag atcaatgatg atgaagtgat ggattatcaa tggtgtgatt
     8101 tagcagatgt attacacggt attgatgcaa cgccgtgggc gttcagtccg tggatggtga
     8161 tgcaggcgac aaatcgcgaa gccagaaaac gattatctgc atttacccag cttaaataag
     8221 ctttgcctca ggatttttga aatgacggtc tgcgcaaaaa aacacgttca tctcactcgc
     8281 gatgctgcgg agcagttact ggctgatatt gatcgacgcc ttgatcagtt attgcccgtg
     8341 gagggagaac gggatgttgt gggtgccgcg atgcgtgaag gtgcgctggc accgggaaaa
     8401 cgtattcgcc ccatgttgct gttgctgacc gcccgcgatc tgggttgcgc tttcagccat
     8461 gacggattac tggatttggc ctgtgcggtg gaaatggtcc acgcggcttc gctgatcctt
     8521 gacgatatgc cctgcatgga cgatgcgaag ctgcggcgcg gacgccctac cattcattct
     8581 cattacggag agcatgtggc aatactggcg gcggttgcct tgctgagtaa agcctttggc
     8641 gtaattgccg atgcagatgg cctcacgccg ctggcaaaaa atcgggcggt ttctgaactg
     8701 tcaaacgcca tcggcatgca aggattggtt cagggtcagt tcaaggatct gtctgaaggg
     8761 gataagccgc gcagcgctga agctattttg atgacgaatc actttaaaac cagcacgctg
     8821 ttttgtgcct cgatgcagat ggcctcgatt gttgcgaatg cctccagcga agcgcgtgat
     8881 tgcctgcatc gtttttcact tgatcttggt caggcatttc aactgctgga cgatttgacc
     8941 gatggcatga ccgacaccgg taaggatagc aatcaggacg ccggtaaatc gacgctggtc
     9001 aatctgttag gcccgagggc ggttgaagaa cgtctgagac aacatcttca gcttgccagt
     9061 gagcatctct ctgcggcctg ccaacacggg cacgccactc aacattttat tcaggcctgg
     9121 tttgacaaaa aactcgctgc cgtcagttaa gcttgcaaga ctagcataac cccttggggc
     9181 ctctaaacgg gtcttgaggg gttttttggt gacggtctca tcgatgagac caagtctccg
     9241 gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
     9301 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9481 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctacc
    10021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10081 gatcctttga tctttt
//
