LOCUS       sequence-418270-       14362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for 
            integration-free naive reset.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14362)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 14362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pCXLE-Sox2-17-T2A-Klf4-E2A-Myc
FEATURES             Location/Qualifiers
     source          1..14362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(79..99)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(497..508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(705..724)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    770..825
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(824..843)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1186..1202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1186..1202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1199..1221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1210..1226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1234..1240,1241..1258,1259..1264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1279..1300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    1389..1422
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1883..3808
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    3976..4009
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4196..5985
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6526..6543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6697..7285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6777..6796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7456..8247,8248..8316))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8079..8098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8317..8421)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8452..8831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8833..9115
                     /label=chicken beta-actin promoter
     intron          9116..10132
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10056..10078
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10140..10159
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             11393..11446
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             12917..12976
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 aagcttcggg gactagtcat atgataatca acctctggat tacaaaattt gtgaaagatt
       61 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
      181 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
      241 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
      301 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
      361 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
      421 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
      481 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
      541 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
      601 ggccgcctcc ccgcatcggt acaattcact cctcaggtgc aggctgccta tcagaaggtg
      661 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
      721 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      781 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      841 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
      901 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
      961 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     1021 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     1081 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     1141 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     1201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1321 cgctttccag tcgggaaacc tgtcgtgcca gcggatctca attccgatca tattcaataa
     1381 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtctg aagaggagtt
     1441 tacgtccagc caagcttagg atcaattctc atgtttgaca gcttatcatc gataagctga
     1501 tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac
     1561 cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg
     1621 gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg
     1681 gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa
     1741 ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt
     1801 ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta
     1861 ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg
     1921 cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag
     1981 aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag
     2041 accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc
     2101 ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc
     2161 aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag gagcaggagg
     2221 aggggcagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg
     2281 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg
     2341 ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag gagcaggagg
     2401 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
     2461 aggaggggca ggagcaggag gaggggcagg agcaggaggg gcaggagggg caggaggggc
     2521 aggagcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggagcagg
     2581 aggggcagga gcaggagggg caggagcagg aggggcagga gcaggagggg caggaggggc
     2641 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
     2701 aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggggcagg
     2761 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
     2821 aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg gtcgaggagg
     2881 cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta gtggaggccg
     2941 ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca gggggagagg
     3001 tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat catccgggtc
     3061 tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag gggaagccga
     3121 ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc ccccgggagc
     3181 gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac cccggggtca
     3241 gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg
     3301 ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg ctaggagtca
     3361 cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat atggaggtag
     3421 taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac aatgtcgtct
     3481 tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac aacctggccc
     3541 gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata tatttgctga
     3601 ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta cctgcaatat
     3661 cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt ttccacctat
     3721 ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag gaggtgatgg
     3781 agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc tcaatcgtat
     3841 taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc atgcgtgctg
     3901 ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca tggggcaatt
     3961 gccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
     4021 aagaggttca ctagcggatc tcaattgggc atacccatgt tgtcacgtca ctcagctccg
     4081 cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
     4141 atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcagga
     4201 aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg caaaggatag
     4261 cactcccact ctactactgg gtatcatatg ctgactgtat atgcatgagg atagcatatg
     4321 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
     4381 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
     4441 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     4501 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcatatg
     4561 ctatccagat atttgggtag tatatgctac ccagatataa attaggatag catatactac
     4621 cctaatctct attaggatag catatgctac ccggatacag attaggatag catatactac
     4681 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     4741 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
     4801 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctat
     4861 ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga
     4921 cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat
     4981 ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg
     5041 tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg
     5101 gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg
     5161 tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct
     5221 ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac
     5281 cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa
     5341 caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat
     5401 attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc
     5461 tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt
     5521 cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa
     5581 tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt
     5641 tcgagcacct cacatacacc ttactgttca caactcagca gttattctat tagctaaacg
     5701 aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc
     5761 agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa
     5821 ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta
     5881 accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag
     5941 tctggatagt atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg
     6001 ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag
     6061 gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca
     6121 tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg
     6181 ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg
     6241 tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc
     6301 ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat
     6361 tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg
     6421 ggcggggatc gatcctctag agtcgacgct agcggatccg ctgcattaat gaatcggcca
     6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
     8461 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     8521 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     8581 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     8641 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8701 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     8761 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     8821 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     8881 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     8941 gggggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     9001 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     9061 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     9121 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     9181 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     9241 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     9301 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     9361 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     9421 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     9481 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     9541 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     9601 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     9661 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     9721 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     9781 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     9841 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     9901 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     9961 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
    10021 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
    10081 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
    10141 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcgcgg ccgccatgta
    10201 taacatgatg gagacggagc tgaagccgcc gggcccgcag caagcttcgg ggggcggcgg
    10261 cggaggaggc aacgccacgg cggcggcaac cggcggcaac cagaagaaca gcccggaccg
    10321 cgtcaagagg cccatgaacg ccttcatggt atggtcccgg gggcagcggc gtaagatggc
    10381 ccaggagaac cccaagatgc acaactcgga gatcagcaag cgcctgggcg cggagtggaa
    10441 agcgttgacc ttggcagaga agcggccgtt catcgacgag gccaagcggc tgcgcgtgct
    10501 gcatatgcag gaccacccca actacaagta ccggccgcgg cggcgcaagc aggtgaagcg
    10561 catgaagcgg gtggagggag gcttcctgca cgctctcgtc gagccccagg ccggcgcgct
    10621 tggtcccgag ggcggccgcg tggccatgga tggcctgggt ctgcctttcc cggagccggg
    10681 ctatccggcc ggtcctccgc tgatgtctcc gcacatgggc ccccactatc gggactgcca
    10741 gggactgggc gctcccgcgc tcgacggcta ccctctgccc actccggaca catccccgct
    10801 ggatggcgtg gagcaggacc cggctttctt tgcagccccg ctgccagggg actgcccggc
    10861 ggccggcacc tacacttacg ctccagtctc ggactatgca gtgtccgtag agccgcccgc
    10921 tggccccatg cgagtggggc cggacccctc gggccctgcg atgccgggga tcctggcgcc
    10981 ccccagcgct ctgcacctgt actacggcgc gatgggctcg cccgccgcaa gtgcggggcg
    11041 cggtttccac gcgcaacccc agcagccgct gcaaccgcag gcaccgcccc cgccaccgca
    11101 gcagcagcac ccagcgcacg gccccgggca accttcgccc cctcccgagg ctctgccctg
    11161 ccgggatggc acggaatcca accagcccac tgagctccta ggggaggtgg accgcacgga
    11221 atttgaacag tatctgccct ttgtgtataa gcccgagatg ggtcttccct accagggaca
    11281 cgactgcgga gtgaacctct cagacagcca cggagccatt tcctccgtgg tgtccgacgc
    11341 tagctcagcg gtctactatt gcaactaccc cgacattgca tgcggctccg gcgagggcag
    11401 gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc ggcccactcg agatgaggca
    11461 gccacctggc gagtctgaca tggctgtcag cgacgctctg ctcccgtcct tctccacgtt
    11521 cgcgtccggc ccggcgggaa gggagaagac actgcgtcca gcaggtgccc cgactaaccg
    11581 ttggcgtgag gaactctctc acatgaagcg acttccccca cttcccggcc gcccctacga
    11641 cctggcggcg acggtggcca cagacctgga gagtggcgga gctggtgcag cttgcagcag
    11701 taacaacccg gccctcctag cccggaggga gaccgaggag ttcaacgacc tcctggacct
    11761 agactttatc ctttccaact cgctaaccca ccaggaatcg gtggccgcca ccgtgaccac
    11821 ctcggcgtca gcttcatcct cgtcttcccc agcgagcagc ggccctgcca gcgcgccctc
    11881 cacctgcagc ttcagctatc cgatccgggc cgggggtgac ccgggcgtgg ctgccagcaa
    11941 cacaggtgga gggctcctct acagccgaga atctgcgcca cctcccacgg cccccttcaa
    12001 cctggcggac atcaatgacg tgagcccctc gggcggcttc gtggctgagc tcctgcggcc
    12061 ggagttggac ccagtataca ttccgccaca gcagcctcag ccgccaggtg gcgggctgat
    12121 gggcaagttt gtgctgaagg cgtctctgac cacccctggc agcgagtaca gcagcccttc
    12181 ggtcatcagt gttagcaaag gaagcccaga cggcagccac cccgtggtag tggcgcccta
    12241 cagcggtggc ccgccgcgca tgtgccccaa gattaagcaa gaggcggtcc cgtcctgcac
    12301 ggtcagccgg tccctagagg cccatttgag cgctggaccc cagctcagca acggccaccg
    12361 gcccaacaca cacgacttcc ccctggggcg gcagctcccc accaggacta cccctacact
    12421 gagtcccgag gaactgctga acagcaggga ctgtcaccct ggcctgcctc ttcccccagg
    12481 attccatccc catccggggc ccaactaccc tcctttcctg ccagaccaga tgcagtcaca
    12541 agtcccctct ctccattatc aagagctcat gccaccgggt tcctgcctgc cagaggagcc
    12601 caagccaaag aggggaagaa ggtcgtggcc ccggaaaaga acagccaccc acacttgtga
    12661 ctatgcaggc tgtggcaaaa cctataccaa gagttctcat ctcaaggcac acctgcgaac
    12721 tcacacaggc gagaaacctt accactgtga ctgggacggc tgtgggtgga aattcgcccg
    12781 ctccgatgaa ctgaccaggc actaccgcaa acacacaggg caccggccct ttcagtgcca
    12841 gaagtgcgac agggcctttt ccaggtcgga ccaccttgcc ttacacatga agaggcactt
    12901 tctcgagggc tcgggccagt gtactaatta tgctctcttg aaattggctg gagatgttga
    12961 gagcaaccca ggtcccggcg cgccactgga tttcctttgg gcgttggaaa ccccgcagac
    13021 agccacgacg atgcccctca acgtgaactt caccaacagg aactatgacc tcgactacga
    13081 ctccgtacag ccctatttca tctgcgacga ggaagagaat ttctatcacc agcaacagca
    13141 gagcgagctg cagccgcccg cgcccagtga ggatatctgg aagaaattcg agctgcttcc
    13201 caccccgccc ctgtccccga gccgccgctc cgggctctgc tctccatcct atgttgcggt
    13261 cgctacgtcc ttctccccaa gggaagacga tgacggcggc ggtggcaact tctccaccgc
    13321 cgatcagctg gagatgatga ccgagttact tggaggagac atggtgaacc agagcttcat
    13381 ctgcgatcct gacgacgaga ccttcatcaa gaacatcatc atccaggact gtatgtggag
    13441 cggtttctca gccgctgcca agctggtctc ggagaagctg gcctcctacc aggctgcgcg
    13501 caaagacagc accagcctga gccccgcccg cgggcacagc gtctgctcca cctccagcct
    13561 gtacctgcag gacctcaccg ccgccgcgtc cgagtgcatt gacccctcag tggtctttcc
    13621 ctacccgctc aacgacagca gctcgcccaa atcctgtacc tcgtccgatt ccacggcctt
    13681 ctctccttcc tcggactcgc tgctgtcctc cgagtcctcc ccacgggcca gccctgagcc
    13741 cctagtgctg catgaggaga caccgcccac caccagcagc gactctgaag aagagcaaga
    13801 agatgaggaa gaaattgatg tggtgtctgt ggagaagagg caaacccctg ccaagaggtc
    13861 ggagtcgggc tcatctccat cccgaggcca cagcaaacct ccgcacagcc cactggtcct
    13921 caagaggtgc cacgtctcca ctcaccagca caactacgcc gcacccccct ccacaaggaa
    13981 ggactatcca gctgccaaga gggccaagtt ggacagtggc agggtcctga agcagatcag
    14041 caacaaccgc aagtgctcca gccccaggtc ctcagacacg gaggaaaacg acaagaggcg
    14101 gacacacaac gtcttggaac gtcagaggag gaacgagctg aagcgcagct tttttgccct
    14161 gcgtgaccag atccctgaat tggaaaacaa cgaaaaggcc cccaaggtag tgatcctcaa
    14221 aaaagccacc gcctacatcc tgtccattca agcagacgag cacaagctca cctctgaaaa
    14281 ggacttattg aggaaacgac gagaacagtt gaaacacaaa ctcgaacagc ttcgaaactc
    14341 tggtgcataa atcgaagaat tc
//
