LOCUS       sequence-418271-        9412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of anti-NPY2R/Neuropeptide Y receptor 
            type 2 (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma
            L119/106..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-NPY2R [L119/106R-2b]
FEATURES             Location/Qualifiers
     source          1..9412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2387..2690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2691..2894
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    4909..4964
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4963..4982)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7200..7218)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7286..7390
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7391..7459,7460..8251)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7609..7628)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8422..9010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8911..8930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9164..9181
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9298..9319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9334..9339,9340..9357,9358..9364)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9372..9388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9377..9399
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9396..9412
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9396..9412
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcacaaattg ttctcaccca gtctccagca atcatgtctg
     1501 catttctagg ggaacgggtc accatgacct gcactgcctc ctcaagtgta cgttccagtc
     1561 acttgtattg gttccagcag cagccaggat cctcccccaa actctggatt tataactcat
     1621 ccaacctggc ttctggagtc ccacctcgct tcagtggcag tgggtctggg acctcttact
     1681 ctctcacaat cagcagcatg gaggctgaag atgctgccac ttattactgc caccaatttc
     1741 atcgttcccc acggacgttc ggtggaggca ccaagctgga aatcaaacgg gctcgggctg
     1801 atgctgcacc aactgtatcc atcttcccac catccagtga gcagttaaca tctggaggtg
     1861 cctcagtcgt gtgcttcttg aacaacttct accccaaaga catcaatgtc aagtggaaga
     1921 ttgatggcag tgaacgacaa aatggcgtcc tgaacagttg gactgatcag gacagcaaag
     1981 acagcaccta cagcatgagc agcaccctca cgttgaccaa ggacgagtat gaacgacata
     2041 acagctatac ctgtgaggcc actcacaaga catcaacttc acccattgtc aagagcttca
     2101 acaggaatga gtgttagaga caaaggtcct gagacgccac caccagctcc ccagctccat
     2161 cctatcttcc cttctaaggt cttggaggct tccccacaag cgacctacca ctgttgcggt
     2221 gctccaaacc tcctccccac ctccttctcc tcctcctccc tttccttggc ttttatcatg
     2281 ctaatatttg cagaaaatat tcaataaagt gagtctttgc acttgatagt tattaatagt
     2341 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2401 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2461 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2521 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     2581 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     2641 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2701 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2761 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2821 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2881 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
     2941 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
     3001 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     3061 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     3121 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     3181 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     3241 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     3301 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     3361 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     3421 ctcccaggcc accatgaagc tgcctgttct gctagtggtg ctgctattgt tcacgagtcc
     3481 agcctcaagc agtgaggtcc agctgcaaca gtctggacct gagctggtga agcctggggc
     3541 ttcagtgaag atatcctgca agacttctgg atacatattc actgaataca ccatacactg
     3601 ggtgaagcag agccttggaa agagccttga gtggattgga ggtcttaatc ctaacaatgg
     3661 tgacactgcc cacaatcaga agttcaaggg caaggccaca ttgtctgtag acaagtcctc
     3721 cagcacagcc tacatggagc tccgcagcct gacatctgag gattctgcag tctatttctg
     3781 tgcaagtttc tactatggta acggaattct tgactactgg ggccaaggca ccactctcac
     3841 agtctcctca gcgcgcccaa cacccccctc agtgtacccc cttgctccag ggtgcggcga
     3901 cactactgga tctagcgtta cactgggctg tttggtgaaa gggtattttc cagagtcagt
     3961 tactgtaacc tggaacagcg gaagtctttc ttcaagtgtg catacattcc ctgctttgct
     4021 tcagtccggc ctgtacacca tgtcttcttc tgtgactgtg cctagttcca cctggccatc
     4081 ccagactgtc acatgctcag tggcccatcc agccagcagc acaaccgtgg acaagaaact
     4141 agagccatca ggtcccatca gcacgataaa cccatgccca ccctgcaagg aatgtcacaa
     4201 atgccctgca ccaaacctgg aagggggtcc ttccgtgttc atatttccac cgaatattaa
     4261 agatgtcctc atgattagcc tcacccccaa agtgacatgt gtagttgtag atgtttcgga
     4321 ggatgatcct gatgtccaga tctcctggtt tgtaaacaat gtcgaagtcc acaccgccca
     4381 gacgcaaaca cacagagaag actacaacag caccatccga gtggtcagca cactcccgat
     4441 tcagcaccag gactggatga gcggtaagga gttcaagtgt aaagtcaaca acaaggatct
     4501 gcctagtcct attgaaagaa ccatcagtaa gatcaaagga ctggtgaggg ccccgcaagt
     4561 ttatatcctg cctcctcccg ccgagcagtt atctcgcaag gacgtttccc tcacttgtct
     4621 ggtagtaggc tttaaccctg gcgacatctc cgtggagtgg acgagcaatg ggcacacaga
     4681 ggagaactac aaggacactg cgcccgtgct agattcagac ggctcttact tcatatatag
     4741 caaactgaat atgaaaacca gcaagtggga gaagacagac tctttctcct gcaatgtgag
     4801 gcatgaagga ttgaagaatt attacctgaa gaagaccatt tcacggagtc ccggaaaatg
     4861 atctagacac gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat
     4921 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     4981 ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg
     5041 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     5101 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     5161 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     5221 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     5281 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     5341 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     5401 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     5461 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     5521 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     5581 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     5641 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     5701 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     5761 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     5821 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     5881 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     5941 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     6001 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     6061 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     6121 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     6181 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     6241 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     6301 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     6361 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     6421 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     6481 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     6541 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     6601 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     6661 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     6721 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     6781 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
     6841 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
     6901 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6961 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     7021 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     7081 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     7141 agcatatgct atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg
     7201 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7261 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7321 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7381 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7441 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7501 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7561 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7621 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7681 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7741 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7801 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7861 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7921 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7981 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8041 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8101 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8161 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8221 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8281 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8341 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8401 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8461 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8521 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8581 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8641 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8701 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8761 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8821 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8881 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8941 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9001 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9061 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9121 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9181 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9241 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9301 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9361 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg ac
//
