LOCUS       sequence-418274-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of anti-Pan-Na+ channel (Human) 
            subclass-switched IgG2b R-mAb. Derived from hybridoma K58/35..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-Pan-Na+ channel [K58/35R-2b]
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2378..2681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2682..2885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    4906..4961
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4960..4979)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7197..7215)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7283..7387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7388..7456,7457..8248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7606..7625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8419..9007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8908..8927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9161..9178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9295..9316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9331..9336,9337..9354,9355..9361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9369..9385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9374..9396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9393..9409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9393..9409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcagaaattg tgctaactca gtctccaaaa ttcatgtcca
     1501 catcagtagg agacagagtc agcgtcacct gcaaggccag tcagaatgtg gatactgatg
     1561 tagcctggta tcaacagaaa gtagggcaat ctcctaaagc aatgatttat tcggcatcct
     1621 accggtacag tgaagtccct gatcgcttca caggcagtgg atctgggaca gatttcactc
     1681 tcaccatcag caatgtgcag tctgaagact tggcagagta tttctgtcag caatataaca
     1741 gccatcctct cacgttcgga ggggggacca agctggaaat aaaacgggct gatgctgcac
     1801 caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt gcctcagtcg
     1861 tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag attgatggca
     1921 gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa gacagcacct
     1981 acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat aacagctata
     2041 cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc aacaggaatg
     2101 agtgttagag acaaaggtcc tgagacgcca ccaccagctc cccagctcca tcctatcttc
     2161 ccttctaagg tcttggaggc ttccccacaa gcgacctacc actgttgcgg tgctccaaac
     2221 ctcctcccca cctccttctc ctcctcctcc ctttccttgg cttttatcat gctaatattt
     2281 gcagaaaata ttcaataaag tgagtctttg cacttgatag ttattaatag taatcaatta
     2341 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2401 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2461 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2521 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     2581 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     2641 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2701 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2761 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     2821 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2881 gagctcgttt agtgaaccgt cagatcctca ctctcttccg catcgctgtc tgcgagggcc
     2941 agctgttggg ctcgcggttg aggacaaact cttcgcggtc tttccagtac tcttggatcg
     3001 gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg acctgagcga gtccgcatcg
     3061 accggatcgg aaaacctctc gagaaaggcg tctaaccagt cacagtcgca aggtaggctg
     3121 agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg
     3181 atgatgtaat taaagtaggc ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg
     3241 cttgagatcc agctgttggg gtgagtactc cctctcaaaa gcgggcatta cttctgcgct
     3301 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg atctggccat
     3361 acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     3421 caccatgaag ctgcctgttc tgctagtggt gctgctattg ttcacgagtc cagcctcaag
     3481 cagtcaggtg cagctgaagc agtctggggc tgagcttgtg aggccagggg ccttagtcaa
     3541 gttgtcctgc acagcttctg gcttcaacat tagagactac tatatgcact ggatgagaca
     3601 gaggcctgag cagggcctgg agtggattgg atggattgat cttgagaatg gtaatgctat
     3661 atttgacccg aagttccagg acaaggccac tatgacactt gacacatcct ccatcacagc
     3721 cttcctgcac ctcagcagcc tgacatctga ggacactgcc gtctattact gtgccgtcct
     3781 cttctatggt aacttcccct atgctatgga ctactggggt caaggaacct cagtcaccgt
     3841 ctcctcggcg cgcccaacac ccccctcagt gtaccccctt gctccagggt gcggcgacac
     3901 tactggatct agcgttacac tgggctgttt ggtgaaaggg tattttccag agtcagttac
     3961 tgtaacctgg aacagcggaa gtctttcttc aagtgtgcat acattccctg ctttgcttca
     4021 gtccggcctg tacaccatgt cttcttctgt gactgtgcct agttccacct ggccatccca
     4081 gactgtcaca tgctcagtgg cccatccagc cagcagcaca accgtggaca agaaactaga
     4141 gccatcaggt cccatcagca cgataaaccc atgcccaccc tgcaaggaat gtcacaaatg
     4201 ccctgcacca aacctggaag ggggtccttc cgtgttcata tttccaccga atattaaaga
     4261 tgtcctcatg attagcctca cccccaaagt gacatgtgta gttgtagatg tttcggagga
     4321 tgatcctgat gtccagatct cctggtttgt aaacaatgtc gaagtccaca ccgcccagac
     4381 gcaaacacac agagaagact acaacagcac catccgagtg gtcagcacac tcccgattca
     4441 gcaccaggac tggatgagcg gtaaggagtt caagtgtaaa gtcaacaaca aggatctgcc
     4501 tagtcctatt gaaagaacca tcagtaagat caaaggactg gtgagggccc cgcaagttta
     4561 tatcctgcct cctcccgccg agcagttatc tcgcaaggac gtttccctca cttgtctggt
     4621 agtaggcttt aaccctggcg acatctccgt ggagtggacg agcaatgggc acacagagga
     4681 gaactacaag gacactgcgc ccgtgctaga ttcagacggc tcttacttca tatatagcaa
     4741 actgaatatg aaaaccagca agtgggagaa gacagactct ttctcctgca atgtgaggca
     4801 tgaaggattg aagaattatt acctgaagaa gaccatttca cggagtcccg gaaaatgatc
     4861 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     4921 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4981 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     5041 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     5101 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     5161 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     5221 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     5281 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     5341 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     5401 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     5461 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     5521 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     5581 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     5641 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     5701 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     5761 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     5821 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     5881 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     5941 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     6001 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     6061 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     6121 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     6181 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     6241 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     6301 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     6361 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     6421 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     6481 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     6541 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     6601 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     6661 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     6721 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     6781 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     6841 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     6901 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     6961 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     7021 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     7081 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     7141 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     7201 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7261 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7321 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7381 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7441 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7501 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7561 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7621 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7681 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7741 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7801 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7861 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7921 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7981 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8041 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8101 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8161 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8221 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8521 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9181 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9241 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9301 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9361 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgac
//
