LOCUS       sequence-418278-        9418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of anti-Pan-MAGUK (Human) 
            subclass-switched IgG2b R-mAb. Derived from hybridoma K28/86..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-Pan-MAGUK [K28/86R-2b]
FEATURES             Location/Qualifiers
     source          1..9418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2396..2699
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2700..2903
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    4915..4970
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4969..4988)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7206..7224)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7292..7396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7397..7465,7466..8257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7615..7634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8428..9016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8917..8936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9170..9187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9304..9325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9340..9345,9346..9363,9364..9370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9378..9394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9383..9405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9402..9418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9402..9418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcagacattg tgctgacaca gtctcctgct tctttagttg
     1501 tatctctggg gcagagggcc accatctcat gcagggccag tcaaagtgtc agtaagtcta
     1561 cctatagtta tctgcactgg taccaacaga aaccaggaca gccacccaaa ctcctcatca
     1621 agtatgcatc caacctagaa tctggtgtcc ctgccaggtt cagtggcagt gggtctggga
     1681 cagacttcat cctcaacatc catcctgtgg aggaggagga tactacaacc tattactgtc
     1741 agcacagttg ggagattcct tggacgttcg gtggaggcac caagctggaa atcaaacggg
     1801 ctcgggctga tgctgcacca actgtatcca tcttcccacc atccagtgag cagttaacat
     1861 ctggaggtgc ctcagtcgtg tgcttcttga acaacttcta ccccaaagac atcaatgtca
     1921 agtggaagat tgatggcagt gaacgacaaa atggcgtcct gaacagttgg actgatcagg
     1981 acagcaaaga cagcacctac agcatgagca gcaccctcac gttgaccaag gacgagtatg
     2041 aacgacataa cagctatacc tgtgaggcca ctcacaagac atcaacttca cccattgtca
     2101 agagcttcaa caggaatgag tgttagagac aaaggtcctg agacgccacc accagctccc
     2161 cagctccatc ctatcttccc ttctaaggtc ttggaggctt ccccacaagc gacctaccac
     2221 tgttgcggtg ctccaaacct cctccccacc tccttctcct cctcctccct ttccttggct
     2281 tttatcatgc taatatttgc agaaaatatt caataaagtg agtctttgca cttgatagtt
     2341 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2401 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2461 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2521 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     2581 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2641 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2701 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     2761 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2821 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     2881 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcctcact ctcttccgca
     2941 tcgctgtctg cgagggccag ctgttgggct cgcggttgag gacaaactct tcgcggtctt
     3001 tccagtactc ttggatcgga aacccgtcgg cctccgaacg gtactccgcc accgagggac
     3061 ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca
     3121 cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggtggcggtc ggggttgttt
     3181 ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc
     3241 gaggtgaggt gtggcaggct tgagatccag ctgttggggt gagtactccc tctcaaaagc
     3301 gggcattact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac
     3361 ctggcccgat ctggccatac acttgagtga caatgacatc cactttgcct ttctctccac
     3421 aggtgtccac tcccaggcca ccatgaagct gcctgttctg ctagtggtgc tgctattgtt
     3481 cacgagtcca gcctcaagca gtgatgtaca gcttcaggag tcaggacctg gcctcgtgaa
     3541 accttctcag tctctgtctc tcacctgctc tgtcactggc tactccatca ccagtggtta
     3601 ttactggaac tggatccggc agtttccagg aaacaaactg gaatggatgg gctacataga
     3661 ctacgacggt agcaatgact acaacccttc tctcaaaaat cgaatctcca tcactcgtga
     3721 cacttctaag aaccagtttt tcctgaactt gaattctgtg actactgagg acacagctac
     3781 attttactgt gcaagagatc agggtttcta cgggtttggt tactggggcc aagggactct
     3841 ggtcactgtc tctgcagcgc gcccaacacc cccctcagtg tacccccttg ctccagggtg
     3901 cggcgacact actggatcta gcgttacact gggctgtttg gtgaaagggt attttccaga
     3961 gtcagttact gtaacctgga acagcggaag tctttcttca agtgtgcata cattccctgc
     4021 tttgcttcag tccggcctgt acaccatgtc ttcttctgtg actgtgccta gttccacctg
     4081 gccatcccag actgtcacat gctcagtggc ccatccagcc agcagcacaa ccgtggacaa
     4141 gaaactagag ccatcaggtc ccatcagcac gataaaccca tgcccaccct gcaaggaatg
     4201 tcacaaatgc cctgcaccaa acctggaagg gggtccttcc gtgttcatat ttccaccgaa
     4261 tattaaagat gtcctcatga ttagcctcac ccccaaagtg acatgtgtag ttgtagatgt
     4321 ttcggaggat gatcctgatg tccagatctc ctggtttgta aacaatgtcg aagtccacac
     4381 cgcccagacg caaacacaca gagaagacta caacagcacc atccgagtgg tcagcacact
     4441 cccgattcag caccaggact ggatgagcgg taaggagttc aagtgtaaag tcaacaacaa
     4501 ggatctgcct agtcctattg aaagaaccat cagtaagatc aaaggactgg tgagggcccc
     4561 gcaagtttat atcctgcctc ctcccgccga gcagttatct cgcaaggacg tttccctcac
     4621 ttgtctggta gtaggcttta accctggcga catctccgtg gagtggacga gcaatgggca
     4681 cacagaggag aactacaagg acactgcgcc cgtgctagat tcagacggct cttacttcat
     4741 atatagcaaa ctgaatatga aaaccagcaa gtgggagaag acagactctt tctcctgcaa
     4801 tgtgaggcat gaaggattga agaattatta cctgaagaag accatttcac ggagtcccgg
     4861 aaaatgatct agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa
     4921 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     4981 catatgggag ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga
     5041 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     5101 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     5161 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     5221 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     5281 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     5341 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     5401 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     5461 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     5521 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     5581 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     5641 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     5701 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     5761 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     5821 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     5881 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     5941 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     6001 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     6061 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     6121 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     6181 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     6241 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     6301 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     6361 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     6421 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     6481 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     6541 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     6601 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     6661 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     6721 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     6781 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     6841 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     6901 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     6961 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     7021 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     7081 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     7141 ccgggtagca tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg
     7201 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7261 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7321 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7621 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7921 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     7981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8101 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9121 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9181 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9241 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9301 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9361 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgac
//
