LOCUS       sequence-418288-        8085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express STING for IFN-beta luciferase reporter assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg 
            MC, Picard C, Neven B, Fischer A, Amigorena S, Rieux-Laucat F, Manel
            N
  TITLE     Intrinsic antiproliferative activity of the innate sensor STING in T
            lymphocytes.
  JOURNAL   J Exp Med. 2017 Jun 5;214(6):1769-1785. doi: 10.1084/jem.20161674. 
            Epub 2017 May 8.
  PUBMED    28484079
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20161674"; journalName: "J Exp Med"; date: "2017-06-5- 
            5"; volume: "214"; issue: "6"; pages: "1769-1785"
COMMENT     Sequence Label: pMSCV-hygro-STING
FEATURES             Location/Qualifiers
     source          1..8085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(594..613)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(741..763)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(957..976)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1076..1098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1136..1154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1222..1326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1327..1395,1396..2187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1545..1564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2358..2946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2847..2866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3100..3117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3234..3255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3270..3275,3276..3293,3294..3300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3308..3324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             3486..4000
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(4358..5383)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(4358..5383)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5401..5900)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5449..5468)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5842..5865
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(7075..7491)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7091..7107)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7123..7145)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7558..7899
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             join(7963..8085,1..394)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
       61 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      121 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      181 tgagggcggg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      241 cttaccacag atatcctgtt tggcccatat tctgctgtct ctctgttcct aaccttgatc
      301 tgaacttctc tattctcagt tatgtatttt ccatgccttg caaaatggcg ttacttaagc
      361 tagcttgcca aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa
      421 aatcttttat tttatctatg gctcgtactc tataggcttc agctggtgat attgttgagt
      481 caaaactaga gcctggacca ctgatatcct gtctttaaca aattggacta atcgcctcta
      541 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
      601 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
      661 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
      721 tgcaccattc cttgcgccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      781 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      841 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
      901 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
      961 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     1021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     1141 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1561 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1861 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     3241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     3301 tgtggaattg tgagcggata acaatttcac aattctcccc agctctttct gcgctgtcca
     3361 taatgaacta tttcaggagt attggagtat gtggattcaa aacagtggct aattgagtca
     3421 ggattactta atattcgact cctaccctca gaatgttgtt ctccaacttc aagaaactgt
     3481 tacccatgaa agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga
     3541 acagcgagac cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg
     3601 ggcgacgcag tctatcggag gactggcgcg ccgagtgagg ggttgtgggc tcttttattg
     3661 agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa
     3721 taaggcacag ggtcatttca ggtccttggg gcaccctgga aacatctgat ggttctctag
     3781 aaactgctga gggcgggacc gcatctgggg accatctgtt cttggccctg agccggggca
     3841 ggaactgctt accacagata tcctgtttgg cccatattct gctgtctctc tgttcctaac
     3901 cttgatctga acttctctat tctcagttat gtattttcca tgccttgcaa aatggcgtta
     3961 cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac
     4021 taaataaaat cttttatttt atcgataagc ttggctgcag gtcgacggat ctgcacccaa
     4081 tcggcaggca cgggcggcga tctccaatct gcgggatcag tcagatcacc cgagtgcgtg
     4141 ggcatgacaa tcgtgccctg gggaccaaca caatccagaa gggcctgaat cactgcgacc
     4201 ggccctcccg cgacccagcc gagcgagctt agcgaactgt ggacgagaac tgtgccacca
     4261 agcgtaaggc cgttctctcg catttgcctt gctaggctcg cgcgagttgc tggctgaggc
     4321 gttctcgaaa tcagctcttg ttcggtcggc atctactcta ttcctttgcc ctcggacgag
     4381 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     4441 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     4501 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     4561 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     4621 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     4681 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     4741 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     4801 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     4861 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     4921 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     4981 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     5041 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     5101 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     5161 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     5221 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     5281 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     5341 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catggtggcg ggatgcaggt
     5401 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt
     5461 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct
     5521 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag
     5581 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat
     5641 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg
     5701 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc
     5761 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc
     5821 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg
     5881 gaaaagcgcc tcccctaccc ggtagaattc gttaacctcg agtcaagaga aatccgtgcg
     5941 gagagggagg ggcttttcca ttccactgat gaggagctca ggctcttggg acatcgtgga
     6001 ggtactgggc accgctgagg tcttcaagct gcccacagta acctcttcct tttcctcctg
     6061 ccgcaggtgc cggagaacct cctgggacag cgagaagctg ctgtcatctg caggttcctg
     6121 gtaggcaatg aggcggcagt tgttctgaga ctcaggggca tctgccagga tgtcctcaag
     6181 tgtccggcag aagagtttgg cctgctcaag cctatcctcc cggctaaagc cagcttgact
     6241 gtattgtgac atggcaaaca aagtctgcaa gggggtggcg tactccagga cacaggtgcc
     6301 cgcccgctgc ccgttctcca gaagctcata gatgctgttg ctgtaaaccc gatccttgat
     6361 gccagcacgg tcaccggtct gctggggcag tttatccagg aagcgaatgt tggggtcagc
     6421 catactcagg ttatcaggca ccccacagtc caatgggagg agaatataca gccgctggct
     6481 cactgcaccc cgtagcaggt tgttgtaatg ctgattgtaa gttcgaatcc gggcctggag
     6541 ctctggcagg atcagccgca gatatccgat gtaatatgac catgccagcc catgggccac
     6601 gttgaaattc cctttttcac acactgcaga gatctcagct ggggccaggc ccttgaggcc
     6661 caggaggatg ttcagtgcct gcgagaggcc caggagggca agcatccaag tgaagggcgg
     6721 gccgaccgca tttgggaggg agtagtagaa atagatggac agcagcaaca gggccccacg
     6781 gcggaggggg cagcccaggc aggcccgcac agtcctccag tagctgcccc ggtacctgga
     6841 gtggatgtgg cgcagctcct cagccaggct gcagaccccg tttaacagca gtcccagctg
     6901 cagggaggct aggtggagga ccaggtaccg gagagtgtgc tctggtggct ctcctagccc
     6961 ccaaagggtc accaggcagg cactcagcag aaccaaggct gccttctggg ccccgtgacc
     7021 cctgggacac gggatggatg gatgcaggct ggagtggggc atggatctaa ttccggcgcc
     7081 tagagaagga gtgagggctg gataaaggga ggatcgaggc ggggtcgaac gaggaggttc
     7141 aagggggaga gacggggcgg atggaggaag aggaggcgga ggcttagggt gtacaaaggg
     7201 cttgacccag ggaggggggt caaaagccaa ggcttcccag gtcacgatgt aggggacctg
     7261 gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc
     7321 tcggttaaag gtgccgtctc gcggccatcc gacgttaaag gttggccatt ctgcagagca
     7381 gaaggtaacc caacgtctct tcttgacatc taccgactgg ttgtgagcga tccgctcgac
     7441 atctttccag tgacctaagg tcaaacttaa gggagtggta acagtctggc cctaattttc
     7501 agacaaatac agaaacacag tcagacagag acaacacaga acgatgctgc agcgctgcag
     7561 cagacaagac gcgcggcttc ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa
     7621 ctgttttagg ttctcgtctc ctaccagaac cacatatcct gacggggtcg gattccacat
     7681 cgactccctt cctcaggtcg ggccacaaaa acggccccca aagtccctgg gacgtctccc
     7741 agggttgcgg ccgggtgttc agaactcgtc agttccacca cgggtccgcc agatacagag
     7801 ctagttagct aactagtaca gacgcaggcg caaacattag atgccggcac aaacacgcac
     7861 aaagacagag acagacacga aacgaccgct ggccagctta cctcccggcg ggggggtcgg
     7921 tggtccctgg gcaggggtct ccaaatctcg gtggaacctc caaatgaaag acccccgagg
     7981 tgggcagtca atcaatctga ggagaccctc ccaaggatca gcgagtccac gattcggatg
     8041 caaacagcaa gaggctttat tgggaatacg ggtacccggg cgacg
//
