LOCUS       sequence-418299-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of mTSPAN4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Jiao H, Jiang D, Hu X, Du W, Ji L, Yang Y, Li X, Sho T, Wang X, Li 
            Y, Wu YT, Wei YH, Hu X, Yu L
  TITLE     Mitocytosis, a migrasome-mediated mitochondrial quality-control 
            process.
  JOURNAL   Cell. 2021 May 27;184(11):2896-2910.e13. doi: 
            10.1016/j.cell.2021.04.027.
  PUBMED    34048705
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04"; journalName: "Cell"; date: "2021-05-27- 
            27"; volume: "184"; issue: "11"; pages: "2896-2910.e13"
COMMENT     Sequence Label: pB-mTSPAN4-GFP
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(22..40)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(61..77)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(61..77)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(74..96)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    85..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(109..115,116..133,134..139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(292..309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(463..1051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(543..562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1222..2013,2014..2082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1845..1864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2083..2187)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2213..2668)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2350..2371)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2562..2581
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2795..2817
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2809..2826
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2810..2826
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2833..2852
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2833..2851
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   2870..2904
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(3386..4411)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAGADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4430..4929)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4478..4497)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4871..4894
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    4952..4985
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        5001..5380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6696..6718
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6780..6799
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      6863..6872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7589..7591,7592..7594,7595..8305)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7589..7591,7592..7594,7595..8305)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7634..7655)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7895..7914)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8242..8263
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8352..8369)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8358..8582
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    8667..8784
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8830..8863
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   9034..9096
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aaagagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
       61 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      121 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      181 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2161 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     2221 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2281 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2341 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2401 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2461 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2521 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2581 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2641 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     2701 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     2761 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     2821 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac cctagaaaga
     2881 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     2941 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     3001 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     3061 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     3121 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcca
     3181 actatgaaac attatcataa agcaatagta aagagccttc agtaaagagc aggcatttat
     3241 ctaatcccac cccaccccca cccccgtagc tccaatcctt ccattcaaaa tgtaggtact
     3301 ctgttctcac ccttcttaac aaagtatgac aggaaaaact tccatgtgga catctttatt
     3361 gtttaataga tcatcaattt ctcgactatt cctttgccct cggacgagtg ctggggcgtc
     3421 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     3481 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     3541 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     3601 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
     3661 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     3721 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     3781 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     3841 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     3901 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     3961 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     4021 tcgtctggct aagatcggcc ccagcgatcg catccatggc ctccgcgacc ggctgcagaa
     4081 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     4141 tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg
     4201 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     4261 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     4321 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     4381 cagacgtcgc ggtgagttca ggctttttca tggtggcggc tggatcggtc gaaaggcccg
     4441 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
     4501 ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc
     4561 cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc
     4621 cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac
     4681 tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg
     4741 ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag
     4801 ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
     4861 gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct
     4921 cccctacccg gtagaattca tcgatgatat cataacttcg tataatgtat gctatacgaa
     4981 gttatgcggc cctcgaggtc gacattgatt attgactagt tattaatagt aatcaattac
     5041 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5101 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5161 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5221 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     5281 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     5341 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     5401 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     5461 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc ctgggggggg
     5521 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg ggcgaggggg
     5581 agaggtgggg cggcagcaaa tcagggcggc gcgctccgaa agtttccttt tatggcgagg
     5641 cggggggggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
     5701 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     5761 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     5821 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
     5881 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
     5941 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     6001 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     6061 tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     6121 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     6181 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     6241 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggcgg cgcccgggcg
     6301 gggggggggc gggggggggg gggggggggg gggggggggg gggcgggggg gggggggccc
     6361 gccgggggcg ggggggggct ggggggaggg gcgcggcggc ccccggagcg ccggcggctg
     6421 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     6481 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     6541 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     6601 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     6661 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     6721 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     6781 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcctcg acggtatcga
     6841 taagctatca caagtttgta cagccaccat ggcgcgcggc tgcctccagg gcgtcaagta
     6901 cctcatgttc gccttcaacc tgctcttctg gctgggtggc tgtggtgtcc tgggtgttgg
     6961 catctggttg gctgccacac agggaaactt tgccacctta tcatcctcat ttccatcctt
     7021 gtcggctgcc aacctgctca tcgtcaccgg gaccttcgtc atggccatcg gcttcgtggg
     7081 ctgcattggg gccctcaagg agaacaagtg cctactgctc actttctttg tgctgctgct
     7141 gctagtgttc ctgctggaag ccaccattgc tgtgctcttc tttgcctaca gtgacaagat
     7201 tgacagttat gcccaacaag acctgaagaa gggcctgcat ctgtatggca cacagggcaa
     7261 cgtgggcctc accaatgcct ggagcatcat ccagactgat ttccgatgct gtggagtttc
     7321 caattacact gattggtttg aggtatacaa tgccactcgt gtgcctgact cctgctgtct
     7381 ggagttcagt gatagctgtg ggttacatga acctggtacc tggtggaagt cgccctgtta
     7441 tgagacagtg aaggcctggc tccaggagaa cctgctagct gtgggcatct ttggactgtg
     7501 cacggcactg gtgcagattc tgggcctcac cttcgctatg accatgtact gccaggtggt
     7561 aaaggcggac acctactgtg cacccgggat ggtgagcaag ggcgaggagc tgttcaccgg
     7621 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7681 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7741 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     7801 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7861 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7921 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     7981 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     8041 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     8101 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     8161 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     8221 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     8281 tctcggcatg gacgagctat acaagggatc cacgcgtctc gagttaacgg tacctctaga
     8341 gaattcgcta gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8401 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8461 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8521 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8581 ggcttctgag gcggttaatt aaaataaaac tagagcgggc ccggtactaa ccaaactgga
     8641 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
     8701 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
     8761 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct gcagagcggc
     8821 cgcgtcgaga taacttcgta taatgtatgc tatacgaagt tatgttttgt tactttatag
     8881 aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt
     8941 tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa
     9001 taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc
     9061 tttaacgtac gtcacaatat gattatcttt ctagggtt
//
