LOCUS       sequence-418341-        4734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for in vitro transcription template preparation, insert T7 promoter,
            JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4734)
  TITLE     RBM24 interacts with RPL3 and regulates RPL3-eIF6 interaction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC-T7-J1~398-Luc
FEATURES             Location/Qualifiers
     source          1..4734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             421..2073
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(487..505)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(2117..2133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2117..2133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2130..2152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2141..2157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2165..2171,2172..2189,2190..2195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2210..2231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2348..2365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2519..3107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2599..2618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3278..4069,4070..4138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3901..3920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4139..4243)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4311..4329
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4367..4389)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4489..4508
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4702..4724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4716..4733
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4717..4733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattctaata cgactcacta tagacctgcc cctaataggg gcgacactcc gccatgaatc
       61 actcccctgt gaggaactac tgtcttcacg cagaaagcgc ctagccatgg cgttagtatg
      121 agtgtcgtac agcctccagg cccccccctc ccgggagagc catagtggtc tgcggaaccg
      181 gtgagtacac cggaattgcc gggaagactg ggtcctttct tggataaacc cactctatgc
      241 ccggccattt gggcgtgccc ccgcaagact gctagccgag tagcgttggg ttgcgaaagg
      301 ccttgtggta ctgcctgata gggcgcttgc gagtgccccg ggaggtctcg tagaccgtgc
      361 accatgagca caaatcctaa acctcaaaga aaaaccaaaa gaaacaccaa ccgtcgccca
      421 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
      481 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      541 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      601 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      661 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      721 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
      781 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      841 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
      901 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      961 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     1021 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     1081 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     1141 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     1201 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     1261 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     1321 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     1381 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1441 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1501 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1561 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1621 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1681 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     1741 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     1801 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     1861 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1921 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1981 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     2041 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcgacctgc aggcatgcaa
     2101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2941 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4261 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4321 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4381 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4441 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     4501 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4561 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4621 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     4681 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtg
//
