LOCUS       sequence-41836-e        5537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5537)
  TITLE     pHyVec Cloning Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHyVec11
FEATURES             Location/Qualifiers
     source          1..5537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3349..3369)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3349..3367)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3388..3404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3388..3404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3401..3423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3412..3428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3436..3442,3443..3460,3461..3466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3481..3502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3619..3636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3790..4378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3870..3889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4549..5340,5341..5409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5172..5191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5410..5514)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggttttaag
      661 ccgagatgca ggattctgaa tgaaaaagaa aaaaagaagt ctcggtagag taaaagtgat
      721 cggtttgcaa ctgtaaaatt tattgaagta ccaataattt tatttaaaat aaaactgaaa
      781 tataaagtta aagttgctgt tctataagtt taccgaattt taaaaccatt gtaacgctag
      841 agtaatattt gagtctacta agttagtccc cgcacttttt aatcaagcaa taaataccca
      901 aactttgctt attcaaatca ataaaccaat atatctctta aaataaagta aaaacttctg
      961 aaattctata aaaaaaaatt taatttcgaa atatcaaatg taacttcaac accgcactat
     1021 tttcttttaa acaactgata tagtaattac ttctcaaaaa cgttatctca aggtttgtga
     1081 tgtacttaaa accactccta ttttgttacg cgtttaaaaa agcaaacata agttggtttc
     1141 tattgatgaa tgagaacata tttcatttaa agttaaaatc ctaccagtgg tttcactgta
     1201 cgtaaacacc gtcaaaaaaa caggaacgtt tttaaagatt aataattgaa gtaaaaaaaa
     1261 tttaataccg ggggttaaaa aaatctttta aaataattat aaatatatat attaaaattt
     1321 ataaattttt aaacacattt aaaatatata ttaagtataa taaaagtaat attataaaaa
     1381 aaaatttaat tttataatta tttttattaa atttataaat aataggtaaa acttacatat
     1441 ccgttttatt ttttcttaat aaaataacgc gtgcaaattt ttgtccatat aaagaccttt
     1501 tcgaacaata acttttttgc ttagccgttt tttttcttat atggtcaaaa aagcgctcaa
     1561 gcgattcacc ataaaaagcg caattagttc agcgttcgtt attcagaagc ttcagctttg
     1621 cttgataatc agctcttctc tttttaaaca aaacacttaa tcaagctagc cgatgatgtt
     1681 aacgaggatc cataaatctg attgcgatct tttgttttcc aataaaacaa agataccgaa
     1741 agttaccgat ttttcttacc gaaagtttga caatgctttg ttgttctcca tttttctatt
     1801 gtaattaaaa acattttttc tttttttaag aagttgtgct ttttataaat ttgtgtaaaa
     1861 acgcgttata atgctaagtt gagggttaaa gtttactgac ctgataatat atcgagcgag
     1921 gattattata taattaaaga tttattagta tatatagtat ataacatgta aactgatagt
     1981 atatacttga tatattaatg attaatatat caagcgagga ttcatttatc attaaagtat
     2041 ttgcgatcgt cgcttgtaac attaaatttt aaattttacc ttattaaaac ttcataattt
     2101 tttttatttt tagatagtaa atatagtagc aatggcagat gctgcagaaa tggcttcatc
     2161 agaaaatgtt attacagaat ttatgagatt taaagttaga atggaaggta cagttaatgg
     2221 acatgaattt gaaattgagg gagaaggaga aggtagacca tatgaaggac ataatacagt
     2281 taaattaaag gttacaaaag gaggaccatt accatttgcg tgggatattt tatcaccaca
     2341 atttcaatat ggatcaaagg tttacgttaa acatccagct gatattccag attataaaaa
     2401 attatctttt ccagaaggat ttaaatggga aagagttatg aattttgaag atggtggagt
     2461 tgctacagtt acacaagatt catcattaca agatggttgt tttatttata aagttaaatt
     2521 tattggggtt aattttccat cagacggacc agttatgcaa aaaaaaacaa tgggatggga
     2581 agctagtaca gaaagattat atccaagaga cggggtttta aagggagaaa cacataaagc
     2641 tctaaaatta aaggacggag gacattattt agttgaattt aaatcaattt atatggctaa
     2701 aaaaccagtt caattaccag gatattatta tgtagatgct aagttagata ttacatcaca
     2761 taatgaagat tatacaattg ttgaacaata tgaaagaaca gaaggtagac atcatttatt
     2821 tttatagcat tcgtagattc caagggcgaa ttcacaattc gattatattt atactggact
     2881 atttttacat ctgttcggtt attttcacat ttatttttct atatatatct tataaacgtt
     2941 ttaaaaccca tgtaattttt gttaagctgt aatataaaag acgtcctaac aaacttcttt
     3001 tattactgaa tttcctttaa ttataataaa taacaagttt taaaataaat tcaggcaatt
     3061 aaggcgctcc tgaggtacta aaattaatgt aaacatttaa aattaacttg gatggtctta
     3121 agtactgtac tcgtgatttt gttatacttt attattagaa aagtcgtcta ttaacttttt
     3181 gttccttaat ttacttgatt aaattgtcgc ttaatttatc aaatcaggtt ttgcgcgtta
     3241 ttttagagaa aaacttatta gaaaaatgaa taagcaaagt ttaggctaac atgttttttt
     3301 attattttaa atagttcaag tcaatgacgt ataaaccagc ttttgttccc tttagtgagg
     3361 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3421 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3481 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4201 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     4261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4501 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4621 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4741 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4861 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4921 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4981 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5041 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5101 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5161 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5221 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5281 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5401 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5461 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5521 tccccgaaaa gtgccac
//
