LOCUS       sequence-418362-       12733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA3.1 based empty vector for transient transfection of 
            sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 
            (Addgene#52961), lenti virus sequences not included..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12733)
  TITLE     RBM24 interacts with RPL3 and regulates RPL3-eIF6 interaction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-CRISPR-Puro
FEATURES             Location/Qualifiers
     source          1..12733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        959..1207
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     959..979
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1130..1149
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3089..3164
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3226..3437
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3456..3465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3462..7565
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3462..7565
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7566..7613
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7614..7637
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7647..7703
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7704..8300
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8197..8217
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8324..8341)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8330..8554
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8600..9028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8687..8706)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8897..8918
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9037..9057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9042..9371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9222..9357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9284..9303
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9438..10232
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9492..9511)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10102..10121
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10406..10527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10443..10462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10497..10516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10576..10592)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10576..10592)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10589..10611)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10600..10616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10624..10630,10631..10648,10649..10654))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10669..10690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10807..10824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10978..11566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11058..11077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11737..12528,12529..12597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12360..12379
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12598..12702)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagctca agcttcgaat tctgcagtcg acggtaccga
      961 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     1021 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     1081 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     1141 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     1201 aaacaccgga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
     1261 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
     1321 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
     1381 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
     1441 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
     1501 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
     1561 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
     1621 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
     1681 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
     1741 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
     1801 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
     1861 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
     1921 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
     1981 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
     2041 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
     2101 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
     2161 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
     2221 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
     2281 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
     2341 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
     2401 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
     2461 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
     2521 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
     2581 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
     2641 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
     2701 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
     2761 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
     2821 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
     2881 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
     2941 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
     3001 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
     3061 agcttcttgc tcttttttgt acgtctctgt tttagagcta gaaatagcaa gttaaaataa
     3121 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gaattcgcta
     3181 gctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     3241 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
     3301 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     3361 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     3421 ttgccgccag aacacaggac cggttctaga gcgctgccac catggacaag aagtacagca
     3481 tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
     3541 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
     3601 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
     3661 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
     3721 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
     3781 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
     3841 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
     3901 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
     3961 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
     4021 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
     4081 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg
     4141 cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg
     4201 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga
     4261 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg
     4321 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
     4381 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg
     4441 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt
     4501 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag
     4561 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg
     4621 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca
     4681 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg
     4741 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc
     4801 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca
     4861 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt
     4921 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg
     4981 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag
     5041 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg
     5101 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg
     5161 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt
     5221 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc
     5281 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg
     5341 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag
     5401 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga
     5461 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg
     5521 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
     5581 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc
     5641 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc
     5701 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga
     5761 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
     5821 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
     5881 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg
     5941 aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc
     6001 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga
     6061 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc
     6121 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg
     6181 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
     6241 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg
     6301 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc
     6361 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg
     6421 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg
     6481 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
     6541 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca
     6601 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg
     6661 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc
     6721 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
     6781 aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg
     6841 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg
     6901 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
     6961 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
     7021 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
     7081 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg
     7141 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
     7201 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg
     7261 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
     7321 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg
     7381 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact
     7441 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc
     7501 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
     7561 gcgacaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag aaagattaca
     7621 aagacgatga cgataaggga tccggcgcaa caaacttctc tctgctgaaa caagccggag
     7681 atgtcgaaga gaatcctgga ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7741 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7801 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7861 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7921 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7981 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     8041 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc
     8101 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8161 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8221 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8281 cccgcaagcc cggtgcctga cttaagttta aaccgctgat cagcctcgac tgtgccttct
     8341 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8401 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8461 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8521 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8581 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8641 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8701 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8761 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8821 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8881 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8941 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9001 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     9061 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9121 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9181 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     9241 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9301 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9361 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     9421 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     9481 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     9541 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     9601 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     9661 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     9721 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     9781 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     9841 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     9901 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     9961 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
    10021 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
    10081 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
    10141 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
    10201 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
    10261 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
    10321 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
    10381 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
    10441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10501 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
    10561 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10621 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10681 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10741 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11041 aaacccggca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12721 gccacctgac gtc
//
