LOCUS       sequence-418372-        9346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of anti-KCNT1/Slo2.2/Slack K+ channel 
            (Rat) subclass-switched IgG1 R-mAb. Derived from hybridoma N3/26..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R-1]
FEATURES             Location/Qualifiers
     source          1..9346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2378..2681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2682..2885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    4843..4898
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4897..4916)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7134..7152)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7220..7324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7325..7393,7394..8185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7543..7562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8356..8944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8845..8864
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9098..9115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9232..9253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9268..9273,9274..9291,9292..9298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9306..9322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9311..9333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9330..9346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9330..9346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcagacattg tgatgaccca gactcccaaa ttcctgcttg
     1501 tatcagcagg agacagggtt accatcacct gcaaggccag tcagagtgtg agtaatgaag
     1561 taggttggta ccaacagaag ccagggcagt ctcctaaatt gctgatatac tttgcatcca
     1621 atcgctacac tggagtccct gatcgcttca ctggcagtgg atatgggacg gatttcactt
     1681 tcaccatcag cactgtgcag gctgaagacc tggcagttta tttctgtcag caggattata
     1741 gctctccgtt cacgttcgga ggggggacca agctggaaat aaaacgggct gatgctgcac
     1801 caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt gcctcagtcg
     1861 tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag attgatggca
     1921 gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa gacagcacct
     1981 acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat aacagctata
     2041 cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc aacaggaatg
     2101 agtgttagag acaaaggtcc tgagacgcca ccaccagctc cccagctcca tcctatcttc
     2161 ccttctaagg tcttggaggc ttccccacaa gcgacctacc actgttgcgg tgctccaaac
     2221 ctcctcccca cctccttctc ctcctcctcc ctttccttgg cttttatcat gctaatattt
     2281 gcagaaaata ttcaataaag tgagtctttg cacttgatag ttattaatag taatcaatta
     2341 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2401 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2461 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2521 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     2581 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     2641 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2701 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2761 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     2821 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2881 gagctcgttt agtgaaccgt cagatcctca ctctcttccg catcgctgtc tgcgagggcc
     2941 agctgttggg ctcgcggttg aggacaaact cttcgcggtc tttccagtac tcttggatcg
     3001 gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg acctgagcga gtccgcatcg
     3061 accggatcgg aaaacctctc gagaaaggcg tctaaccagt cacagtcgca aggtaggctg
     3121 agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg
     3181 atgatgtaat taaagtaggc ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg
     3241 cttgagatcc agctgttggg gtgagtactc cctctcaaaa gcgggcatta cttctgcgct
     3301 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg atctggccat
     3361 acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     3421 caccatgaag ctgcctgttc tgctagtggt gctgctattg ttcacgagtc cagcctcaag
     3481 cagtcaggtt cagctgcagc agtcgggacc tggcctggtg aaaccttctc agtctctgtc
     3541 cctcacctgc actgtcactg gctactcaat caccaatgat tatgcctgga actggatccg
     3601 gcagtttcca ggaaacaaac tggagtggat gggctacata aactacagtg gtagcactag
     3661 ctacaacccc tctctcaaaa gtcgaatctc tatcactcga gacacatcca agaaccagtt
     3721 cttcctgcag ttgaattctg tgactactga ggacacagcc acatattact gtgcaagata
     3781 tggttactgg ggccaaggca ccactctcac agtctcctcg gcgcgcccga cacccccatc
     3841 tgtctatcca ctggcccctg gatctgctgc ccaaactaac tccatggtga ccctgggatg
     3901 cctggtcaag ggctatttcc ctgagccagt gacagtgacc tggaactctg gatccctgtc
     3961 cagcggtgtg cacaccttcc cagctgtcct gcagtctgac ctctacactc tgagcagctc
     4021 agtgactgtc ccctccagca cctggcccag cgagaccgtc acctgcaacg ttgcccaccc
     4081 ggccagcagc accaaggtgg acaagaaaat tgtgcccagg gattgtggtt gtaagccttg
     4141 catatgtaca gtcccagaag tatcatctgt cttcatcttc cccccaaagc ccaaggatgt
     4201 gctcaccatt actctgactc ctaaggtcac gtgtgttgtg gtagacatca gcaaggatga
     4261 tcccgaggtc cagttcagct ggtttgtaga tgatgtggag gtgcacacag ctcagacgca
     4321 accccgggag gagcagttca acagcacttt ccgctcagtc agtgaacttc ccatcatgca
     4381 ccaggactgg ctcaatggca aggagttcaa atgcagggtc aacagtgcag ctttccctgc
     4441 ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg aaggctccac aggtgtacac
     4501 cattccacct cccaaggagc agatggccaa ggataaagtc agtctgacct gcatgataac
     4561 agacttcttc cctgaagaca ttactgtgga gtggcagtgg aatgggcagc cagcggagaa
     4621 ctacaagaac actcagccca tcatggacac agatggctct tacttcgtct acagcaagct
     4681 caatgtgcag aagagcaact gggaggcagg aaatactttc acctgctctg tgttacatga
     4741 gggcctgcac aaccaccata ctgagaagag cctctcccac tctcctggta aatgatctag
     4801 acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt
     4861 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     4921 caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg
     4981 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     5041 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     5101 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     5161 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     5221 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     5281 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     5341 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     5401 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     5461 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     5521 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     5581 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     5641 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     5701 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     5761 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     5821 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     5881 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     5941 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     6001 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     6061 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     6121 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     6181 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     6241 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     6301 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     6361 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     6421 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     6481 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     6541 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     6601 catatgctat cctaatctat atctgggtag tatatgctat cctaatytat atctgggtag
     6661 yatakgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     6721 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     6781 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     6841 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     6901 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6961 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     7021 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     7081 tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt
     7141 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7201 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7261 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7321 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7381 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7441 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7501 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7561 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7621 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7681 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7741 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7801 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7861 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7921 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7981 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8041 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8101 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8161 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8221 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8281 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8341 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8401 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8461 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8521 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8581 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8641 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8701 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8761 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8821 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8881 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8941 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9001 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9061 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgac
//
