LOCUS       sequence-418379-        8868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CTNNB1_ULK4 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CTNNB1_ULK4
FEATURES             Location/Qualifiers
     source          1..8868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3503..3538)
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     CDS             complement(join(3629..4339,4340..4342,4343..4345))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3671..3692)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4020..4039
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4279..4300
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4342..4351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4427..4630)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4456..4476)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4631..4934
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5191..5235)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5420..5653
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6013..6130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6622..6747
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6794..7051
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7053..7432
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7463..7567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7568..7636,7637..8428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7786..7805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8599..8868,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaat
     1801 ggcccacggc ctgaagaatc tctaaagcca aaggggctac ggcggagtcc gcgaacgacc
     1861 cactgcctgg cgccaagcgc tccagagcac gaagcaagga gcctgcattt ttcaacgact
     1921 ccaaatgttt ttcgttagag gtaatcatgc ggcgcaagat gcgcagcaaa agtttttgtt
     1981 ctttagggtc ttccttactg gtcagcaaat gggcaaaaat ctccacattc tctggggaca
     2041 gtgagtcagg gttctccccg ccatacagct gtaccaaaat agaaagacac ttagagctca
     2101 cgtcgaaaat ctctggatct tcattaggta aaaggggaat caaaaggcta atcaggtctg
     2161 taagcggacg attcaacaga agaaggtcct ccgctgcttg cgggtcttcc cctgatccag
     2221 acttttgcgc ttgcagtgcc aaacggacga taccagacgt gtacgtcaac atgctatgta
     2281 aaatatcaag taacgagaat aaaagtgggg cagccatctc gttattgttc ttgttgtcta
     2341 catccaggca aagtgtcgcc gtttcggtta acaaattaca aatatgtgac acaaggcctt
     2401 gttcgtacag aagctccata ttagagtctt tgcacgcgac aagattcgac aaaagggcaa
     2461 tgacggattg catcgtattc ccaaggatgc tctcttgatg ctctgcctca acgatagcag
     2521 gcttgttgga ggaacatacc gaaagaactt ttaagacacg actcgtggtc cataaaagtt
     2581 tctcgtatgt atacgtacgc atgatattca ctaacgcttg cggccccccg gaagcaagaa
     2641 tgatcaattt ggactcttga tttccgtatg ccaggatctg taagcaatcg gttgtgatag
     2701 ccagaaactt tacattggtc ttgttcagaa gtgccaccat cttttgcagc cccccggcca
     2761 gacgcaccgc catcttcgcg ccttcctgat gaagcaaaag attatgcaaa gtcgtgatag
     2821 cataaaaaag gacggaatct acaggacttc ctaacatttt gaccaaggcg ggaatgcctc
     2881 cggacttaaa gatggccaac agtccttcgc ggtgatgaga caaattatgc aatgttcccg
     2941 cagtgcaacg agcggtttcc acgtcattag tattctgcat cgtacggaca atggcactaa
     3001 ccatctgtgg cgagcgcata attgcatggc gggatgcctc ttttttactt aattgatgta
     3061 ccataaccgc agccttgttt actacaacct gatcctcgtc gttcagaagt ttggtcaatt
     3121 ccggaatcgc acgcgttgcc aactcggcgt cgtcttggta gttgattaag ttcacaaccg
     3181 cgtgtttcaa catctgtgac ggttcagcta agcgttgtac gttggtgggg tgtgccgcgt
     3241 caaattgggt gcttgggatc tgcattccct catccagtgt ctcgggaaac attgcggcac
     3301 gaacacgttg ggcacgggtc atagcgtact gaccgtcaat atcagctact tgctcctgcg
     3361 taaaagactg actgaagccc tgttcccatt cataaagcac ctgcgaggtg tctacgtcct
     3421 cctcttctgg atttccttta ccgctcagcg aaggggcagt cgtggttgcc ccactatgga
     3481 tgccactatc taaataagat tgttgctgcc aatggctcac agcagctttg cggtccggtt
     3541 ccatcgccat atccaattcc ataaggtctg cttgtgtcgc cattccccgg gaagcggccg
     3601 cacttccccc ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc
     3661 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     3721 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     3781 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     3841 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     3901 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     3961 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     4021 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     4081 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     4141 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     4201 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     4261 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     4321 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
     4381 tcgactgcag aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat
     4441 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     4501 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     4561 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     4621 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     4681 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     4741 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     4801 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     4861 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     4921 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     4981 actattaata actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg
     5041 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     5101 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     5161 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     5221 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     5281 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     5341 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     5401 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     5461 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     5521 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     5581 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     5641 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     5701 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     5761 ccaattgtcc ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc
     5821 ctcatcctgt ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat
     5881 gatctttgct tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc
     5941 cccttcacct ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct
     6001 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     6061 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     6121 tcttttaaaa ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg
     6181 atctcttacc tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg
     6241 actttgggga ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt
     6301 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     6361 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     6421 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     6481 ttctaacagg ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     6541 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     6601 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     6661 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     6721 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     6781 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     6841 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     6901 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     6961 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
     7021 ccactcccca gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag
     7081 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     7141 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     7201 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     7261 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     7321 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     7381 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg
     7441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7501 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7561 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7621 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7681 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7741 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7801 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7861 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7921 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7981 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8041 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8101 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8161 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8221 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8281 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8341 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8401 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8461 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8521 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8701 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
