LOCUS       sequence-418380-       13768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by 
            the rhamnose inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Yap ZL, Rahman ASMZ, Hogan AM, Levin DB, Cardona ST
  TITLE     A CRISPR-Cas-associated transposon system for genome editing in 
            Burkholderia cepacia complex species.
  JOURNAL   Appl Environ Microbiol. 2024 Jul 24;90(7):e0069924. doi: 
            10.1128/aem.00699-24. Epub 2024 Jun 13.
  PUBMED    38869300
REFERENCE   2  (bases 1 to 13768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2024-07-24"; volume: "90(7):e0069924. 
            doi"; pages: " 10.1128/aem.00699-24. Epub 2024 Jun 13"
COMMENT     Sequence Label: pRhaCAST
FEATURES             Location/Qualifiers
     source          1..13768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4383..4454
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        4468..4502
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     terminator      4757..4784
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(4850..4867)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5020..5106
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5198..5225
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5691..6353)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      6354..7125
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     primer_bind     8383..8401
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8518..8620
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(9576..9595)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(10407..10509)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(10666..11514)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(11588..12424)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 caggaacgcc cagaagatgc agagaaggac gcaagactta ctttgcgaga actagaacag
       61 ttacttgtgc gttacatcgt agatcgttac aaccaaagta ttgatgcgcg gatgggcgac
      121 caaacgcgct ttgagcgttg ggaagcagga ttgcctacag tgccagtacc aataccagaa
      181 cgagatttgg atatttgttt aatgaagcag tcacggcgca ctgtgcaaag aggtggttgt
      241 ttgcagtttc agaatttaat gtatcggggg gaatatttgg caggttatgc cggagaaact
      301 gtcaacttaa ggtttgaccc cagagacatt acaacaattt tggtttatcg ccaggaaaac
      361 aatcaggaag tatttctgac tcgcgctcac gctcaaggtt tggagacaga gcaactggca
      421 ttagatgagg ctgaggcagc aagtcgcaga ctccgtaccg cagggaaaac tatcagtaac
      481 caatcattat tgcaagaagt tgttgaccgc gatgctcttg tcgctaccaa gaaaagccgt
      541 aaggagcgtc aaaaattgga acagactgtt ttgcgatctg ctgctgttga tgaaagtaat
      601 agagaatcct tgccttctca aatagttgaa ccagatgaag tggaatctac agaaacggtt
      661 cactctcaat acgaagacat tgaggtgtgg gactatgaac aacttcgtga agaatatggg
      721 ttttaaacaa tgacagaagc tcaggcgatc gccaagcagt tgggtggggt aaaaccggat
      781 gatgagtggt tacaagctga aattgctcgt ctcaagggta agagcattgt gcctttacag
      841 caggtaaaaa ctctccatga ttggttagat ggcaagcgca aggcaagaaa atcttgccga
      901 gtagttgggg aatcgagaac tggcaagaca gttgcttgtg atgcctacag atacaggcac
      961 aaacctcagc aggaagctgg acgacctcca actgtgcctg tcgtttatat tcgacctcac
     1021 caaaaatgtg gccccaagga tttgtttaaa aagattactg agtacctcaa gtatcgggta
     1081 acaaaaggga ctgtatctga ttttcgagat aggacgatag aagtactcaa gggttgtggc
     1141 gtagagatgc taattattga tgaagctgac cgtctcaagc ctgaaacttt tgctgatgtg
     1201 cgagatattg ccgaagattt aggaattgct gtggtactgg taggaacaga ccgtttggat
     1261 gcggtaatta agcgggatga gcaggttctc gaacgctttc gggcgcatct tcgctttggt
     1321 aaattgtcgg gagaggattt taagaacacc gtagaaatgt gggaacaaat ggttttgaaa
     1381 ctgccagtat cttctaatct aaagagcaag gagatgctac ggattctcac gtcagcaact
     1441 gaaggctaca ttggtcgcct tgatgagatt cttagggaag ctgcaattcg ttccttatca
     1501 agaggattga agaagattga caaggctgtt ttacaggaag tagctaagga gtacaaatga
     1561 tagaagcacc agatgttaaa ccttggctat tcttgattaa accctatgaa ggggaaagcc
     1621 tgagccactt tcttggcagg ttcagacgtg ccaaccattt atccgcaagt ggattgggta
     1681 ctttggcagg aattggtgct atagtggcac gttgggaaag atttcatttt aatcctcgcc
     1741 ctagtcagca agaattggaa gcgatcgcat ctgtagtaga agtggatgct caaaggttag
     1801 cccagatgtt accgcctgct ggagtgggaa tgcagcatga gccaattcgc ttgtgtgggg
     1861 cttgttatgc cgagtcgcct tgtcaccgaa ttgaatggca gtacaagtcg gtgtggaagt
     1921 gcgatcgcca tcaactcaag attttagcaa agtgtccaaa ctgtcaagca ccttttaaaa
     1981 tgcctgcgct gtgggaggat gggtgctgtc acagatgtag gatgccgttt gcagaaatgg
     2041 caaagctaca gaaggtttga tgataaaacc agaaaaaggt gtgaaattaa ctaagtccct
     2101 gaattgatct ggttgtccaa aaaatttgtg cgatcgcatg gcaagattat tcctactatt
     2161 gatgtggcgt gagtcggata acttgctcta atgggatccc ggttaatacc aatagatcag
     2221 atatgagtaa taaacattac gacctttacc tgtggtattc actgttggca cgacaacacc
     2281 tttatcgcgc caatactgta gctgacgacg cgaacaatta gtgatttgag ccgcttctgt
     2341 gcttgtgtag aattttcctt ccatagaaat catcctacag aagtagtttt ttaaatttac
     2401 attttttaaa agcacattat gtattcatgt gatattattt ttttatgagt caaataacta
     2461 ttcaagctcg acttatttcc tttgaatcaa accgccaaca actctggaag ttgatggcag
     2521 atttaaacac gccgttaatt aacgaactgc tttgccagtt aggtcaacac cccgacttcg
     2581 agaagtggca acaaaagggt aaactcccgt ctaccgttgt gagccagtta tgtcaacctc
     2641 tcaaaactga ccctcgcttt gcaggtcagc ccagccgttt atatatgtcg gcaattcata
     2701 ttgtggacta catctacaag tcctggctgg ctatacagaa acggcttcaa cagcagctag
     2761 atggaaagac gcgctggcta gaaatgctca atagcgatgc tgaattagta gaacttagtg
     2821 gtgacacttt agaggctatt cgtgtcaaag ctgctgaaat tttggcaata gctatgccag
     2881 catctgagtc agatagcgct tcacctaaag ggaaaaaagg taaaaaggag aaaaaaccct
     2941 catcttctag ccctaagcgt agtttatcca agacattatt tgacgcttac caagaaacgg
     3001 aagatatcaa gagccgtagc gccatcagct acctgttaaa aaatggctgc aaacttactg
     3061 acaaagaaga agattcagaa aaatttgcta aacgtcgtcg tcaagttgaa atccaaattc
     3121 aaaggcttac cgaaaagtta ataagtcgga tgcctaaagg tcgagatttg accaatgcta
     3181 aatggttgga gacactcttg actgctacaa ccactgttgc tgaagacaac gcccaagcca
     3241 aacgctggca ggatattctg ttaactcgat caagttctct cccattcccc cttgtttttg
     3301 aaaccaacga ggatatggtt tggtcaaaga atcaaaaggg taggctgtgt gttcacttca
     3361 atggcttaag cgatttaatt tttgaggtgt actgcggcaa tcgtcaactt cactggtttc
     3421 aacgcttcct agaagaccaa cagactaaac gcaaaagcaa aaatcagcat tctagcggct
     3481 tgttcacact cagaaatggt catctagttt ggcttgaagg tgagggtaaa ggggaacctt
     3541 ggaatcttca ccacttgacc ctttactgct gtgttgacaa tcgcttgtgg acagaggagg
     3601 gaacagaaat cgttcgccaa gagaaagcag atgaaattac taaattcatc acaaacatga
     3661 agaagaaaag cgatctaagc gatacacagc aagctttgat tcaacgtaaa caatcaacac
     3721 ttactcgaat aaacaattcc tttgagcgtc ctagccaacc cctttatcaa ggtcaatcac
     3781 acattttggt tggagtaagc ctgggactag aaaaacctgc cacagtagca gtagtagatg
     3841 cgatcgccaa caaagtcttg gcttaccgga gtattaaaca attacttggc gacaattacg
     3901 aactgctaaa tcgccagaga cgacaacagc agtacctatc tcacgaacgc cacaaagcac
     3961 aaaaaaactt ctctcccaat caatttggag catctgagtt agggcaacat atagacagat
     4021 tattagctaa agcaattgta gcgttagcga gaacctacaa agctggcagt attgtcttgc
     4081 ccaagttagg ggatatgcgg gaggttgtcc aaagtgaaat tcaagctata gcagaacaaa
     4141 aatttcccgg ttatattgaa ggtcagcaaa aatatgccaa acagtaccgg gttaatgttc
     4201 atcggtggag ctacggcaga ttaattcaaa gcattcaaag taaagcagct caaacaggaa
     4261 ttgtgattga ggagggaaaa caacctattc gaggtagtcc ccacgacaaa gcaaaggaat
     4321 tagcactttc tgcttacaat ctccgcctaa ctaggcgaag ttaagtaagg atctccaggc
     4381 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     4441 cggtgaacgc tctctactag agagaccctg acagctagct cagtcctagg tataatgcta
     4501 gcatattaat agcgccgcaa ttcatgctgc ttgcagcctc tgaattttgt taaatgaggg
     4561 ttagtttgac tgtataaata cagtcttgct ttctgaccct ggtagctgct caccctgatg
     4621 ctgctgtcaa tagacaggat aggtgcgctc ccagcaataa gggcgcggat gtactgctgt
     4681 agtggctact gaatcacccc cgatcaaggg ggaaccctaa atgggttgaa aggaagagcg
     4741 agatctcgag ctcttcggct caccttcggg tgggcctttc tgcgcagtct agagtcgacc
     4801 tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga
     4861 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg
     4921 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg
     4981 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
     5041 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     5101 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca
     5161 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc
     5221 ctttttgcgt ttctacaaac tcttgttatt ggtcgactta aacgcctggt gctacgcctg
     5281 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
     5341 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
     5401 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
     5461 ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata
     5521 aaaacggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc
     5581 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg aggcggctac
     5641 agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc
     5701 ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa acacggctca
     5761 tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct
     5821 ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc gccggggccg
     5881 gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc
     5941 aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa caccgacagg
     6001 cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta ggccgacacg
     6061 gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa gtccttgact
     6121 gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt ctggctgacc
     6181 accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat tgccgccgtg
     6241 ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag
     6301 tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc catgcttatc
     6361 tccatgcggt aggggtgccg cacggttgcg gcaccatgcg caatcagctg caacttttcg
     6421 gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt caattacaga ttttctttaa
     6481 cctacgcaat gagctattgc ggggggtgcc gcaatgagct gttgcgtacc cccctttttt
     6541 aagttgttga tttttaagtc tttcgcattt cgccctatat ctagttcttt ggtgcccaaa
     6601 gaagggcacc cctgcggggt tcccccacgc cttcggcgcg gctccccctc cggcaaaaag
     6661 tggcccctcc ggggcttgtt gatcgactgc gcggccttcg gccttgccca aggtggcgct
     6721 gcccccttgg aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc
     6781 gcccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
     6841 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
     6901 gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg
     6961 gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg
     7021 gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag
     7081 cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc
     7141 acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa
     7201 gcccgtaggg cgccgcagcc gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga
     7261 gtatactcaa gcattgagtg gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc
     7321 ggttggacac caaaagggag gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa
     7381 gctggcgaaa atgggcaacg tggcggccag tctcaagcac gcctaccgcg agcgcgagac
     7441 gcccaacgct gacgccagca ggacgccaga gaacgagcac tgggcggcca gcagcaccga
     7501 tgaagcgatg ggccgactgc gcgagttgct gccagagaag cggcgcaagg acgctgtgtt
     7561 ggcggtcgag tacgtcatga cggccagccc ggaatggtgg aagtcggcca gccaagaaca
     7621 gcaggcggcg ttcttcgaga aggcgcacaa gtggctggcg gacaagtacg gggcggatcg
     7681 catcgtgacg gccagcatcc accgtgacga aaccagcccg cacatgaccg cgttcgtggt
     7741 gccgctgacg caggacggca ggctgtcggc caaggagttc atcggcaaca aagcgcagat
     7801 gacccgcgac cagaccacgt ttgcggccgc tgtggccgat ctagggctgc aacggggcat
     7861 cgagggcagc aaggcacgtc acacgcgcat tcaggcgttc tacgaggccc tggagcggcc
     7921 accagtgggc cacgtcacca tcagcccgca agcggtcgag ccacgcgcct atgcaccgca
     7981 gggattggcc gaaaagctgg gaatctcaaa gcgcgttgag acgccggaag ccgtggccga
     8041 ccggctgaca aaagcggttc ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc
     8101 gcgtgagatg cgcaagaagg ccgatcaagc ccaagagacg gcccgagacc ttcgggagcg
     8161 cctgaagccc gttctggacg ccctggggcc gttgaatcgg gatatgcagg ccaaggccgc
     8221 cgcgatcatc aaggccgtgg gcgaaaagct gctgacggaa cagcgggaag tccagcgcca
     8281 gaaacaggcc cagcgccagc aggaacgcgg gcgcgcacat ttccccgaaa agtgccacct
     8341 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8401 ccctttcgtc ttcgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc
     8461 tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga
     8521 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     8581 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     8641 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     8701 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttagcttt
     8761 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     8821 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     8881 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     8941 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     9001 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     9061 cgctgacttg acgggacggc ggctttgttg aataaatcga acttttgctg agttgaagga
     9121 tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat
     9181 caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct
     9241 ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc
     9301 tcagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca
     9361 gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg
     9421 ggatgttgtt taaatggctc tgctgtagtg agtggtctac ggggtctgac gctcagtgga
     9481 acgaaatcga tgataagctg tcaaacatga gcagatcctc tacgccggac gcatcgtggc
     9541 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     9601 gagatcctaa gatatctgga caaaaattaa agagacagag ttaacaacaa acacaatgaa
     9661 gaaaacgaaa tcacaattaa aaacactcag aataataatt tgtgaaagta ccccgatatc
     9721 gatctcaacc tggaagattt cgttcagcgt gtgaatgccg atatctcatc gcagtactgt
     9781 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     9841 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     9901 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     9961 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
    10021 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
    10081 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
    10141 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgtaa ttccggatga
    10201 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
    10261 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
    10321 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
    10381 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacaac
    10441 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
    10501 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
    10561 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtaggcg cgccgaagtt
    10621 cctatactta tgcattttct cagacaagaa gtcagaagaa tgcatttaat ctttctgcga
    10681 attgagatga cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa
    10741 atagttacta tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg
    10801 ctggagaaga tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat
    10861 tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca
    10921 aaatttatcc agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc
    10981 cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag
    11041 caacatcacc aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg
    11101 actactttca tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc ccatgctacc
    11161 taagcgccag tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc
    11221 aagattcagc ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac
    11281 ggaagcgtag gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg
    11341 ataagggcga tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca
    11401 aaaatcatgt gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc
    11461 ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc
    11521 tacctcggcc agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag
    11581 attcgcgtta ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa
    11641 actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga
    11701 tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc
    11761 ggttcaggta tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac
    11821 gcagtgaaag agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt
    11881 cctccagcca ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca
    11941 aactgctttt acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg
    12001 agggtaaatc attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct
    12061 gcaatacgct gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca
    12121 gcaactgatt cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg
    12181 tcagacacag attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga
    12241 ccgtgccacc ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt
    12301 cgacaatcac aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc
    12361 ggggttctat cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg
    12421 tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt
    12481 accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga
    12541 attttcagga aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc
    12601 acgttcatct ttccctggtt gccaatggcc cattttcctg gcaaccaggg aaaggtcgcg
    12661 aattcaggcg ctttttagac tggtcgtaag ggagaccaca acggtttccc tctagtgaaa
    12721 ttcagcagga tcacatatga tgaacagtca gcaaaatcct gatttagctg ttcatccctt
    12781 ggcaattcct atggaaggct tactaggaga aagtgctaca actcttgaga agaatgtaat
    12841 tgccacacaa ctctcagagg aagcccaagt aaagctagag gtaatccaaa gtttactgga
    12901 accctgcgat cgcacaactt atgggcaaaa gttgcgggaa gcagcagaga aactaaatgt
    12961 atcgttgcga acggtacaaa ggttggtgaa aaactgggaa caagatggct tagtcggact
    13021 cactcaaaca agtagggctg ataaaggaaa acaccgcatt ggtgagtttt gggaaaactt
    13081 cattaccaaa acctacaagg agggtaacaa gggaagtaaa cgtatgaccc ctaaacaagt
    13141 tgctctcaga gtcgaggcta aagcccgtga attaaaagac tctaagccgc ccaattacaa
    13201 aaccgtgtta cgggtattag cacccatttt ggaaaagcaa caaaaagcca agagtatccg
    13261 cagtcctggt tggagaggaa ctacgctttc ggttaaaacc cgtgaaggaa aagatttatc
    13321 ggttgattac agtaaccatg tttggcaatg tgaccatacc cgcgtggatg tgttgctggt
    13381 agatcaacat ggtgaaattt taagtcgtcc ctggctaaca acagtaattg atacttactc
    13441 tcgttgcatt atgggtatca acttgggctt tgatgcaccc agttctgggg tagtagcatt
    13501 agcgttacgc catgcaattc taccaaagcg ttacggttcc gagtacaaac tgcattgtga
    13561 gtggggaacc tatggaaaac cagaacattt ttatactgat ggcggtaaag actttcgctc
    13621 taaccacttg agtcagattg gggcgcaatt gggatttgtc tgtcatttac gcgatcgccc
    13681 ttctgaaggt ggagtagtag aacgtccctt caaaacatta aatgaccaac tattttcaac
    13741 gcttcctggg tacaccggat ctaatgtg
//
