LOCUS       sequence-418384-       10566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, EBF1_PDGFRB from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_EBF1_PDGFRB
FEATURES             Location/Qualifiers
     source          1..10566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(5327..6037,6038..6040,6041..6043))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5369..5390)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5718..5737
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5977..5998
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6040..6049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6125..6328)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6154..6174)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6329..6632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6889..6933)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7118..7351
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7711..7828
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8320..8445
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8492..8749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8751..9130
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9161..9265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9266..9334,9335..10126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9484..9503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10297..10566,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggtctaca
     1801 ggaagctatc ctctgcttcc gcccgaggcg cagggcaccc cgaatccggc aactgttcca
     1861 gctctggctc cggctccacc tggagctcaa gctggggctc tggctctggt tcgtcctggg
     1921 gctccagggg gctgtcacag gagatggttg aggaggtgtt gacttcattc agggtggagc
     1981 tggctaggct gggggaaccc tccagtgggc cctcgtcagc aacctcgggt ttggggtcag
     2041 gcagggggat gatatagtcg ttgtcaccct cattgggctg cacggcagta tagaggacgg
     2101 agctggtgtc caggggagat cggaggccat ggaacccagg caagcgggcc tgggaccgaa
     2161 ggatggctgg gtggtcactc ctcagaaact cctcatccac ctgctggtac ttctttttgt
     2221 aaccttcgcc caacagtctc tcgagaagca gcaccagctg ggagaagggg ggccgaatct
     2281 caaacttctc ttcccagcac ttctgcatga tctcatagat ctcgtcggag gcatgggcag
     2341 gctgggccat gcggtaaccc cgtttgatgg cattgtagaa ctgctcgttc atgggcagct
     2401 ctgggtaagg ggtgccaccc aaggtgaaga tctcccagag caggatcccg aaggaccaca
     2461 cgtcgctcag ggtggtgtag aggctgttga agatgctctc cggagccatc cactttaaag
     2521 gcaaaaaggt gctgcctttg gagatgtaat tcgagtcccg catgatgtct cgagccaggc
     2581 caaagtcaca gatcttgacc agcttgcctt cacagatgag cacgttccta gccgccaggt
     2641 ctctgtggac gcagttcttg gaggccagaa actccatgcc attggccacc tggtagctga
     2701 agcccacgag gtccatgtag cttagcactg gagactcgtt gatcaaagtt gctcggcagg
     2761 tcctctcagg ggcagaggga acgtagttat cgtaaggggc catgtagttg gaggactcga
     2821 tgtctgcata tttgacgtct cctttcatgt ccagcatggg cacatagtcc accgactcgt
     2881 ccttgctcat gtccatgtag ccaccgtcgc tctccccggt caaggacaca tggctgggca
     2941 gggggagccc aacgggcaga gcattgctgt agagctccgc gctgggcggg cggcgcttgt
     3001 cggagtggtg ctgcaggaag gtgtgtttgt tgcggtgcag gtagtccacc aggtctccgt
     3061 agcggcagta ctcagtgatg atatagatgg gtcctccttt ggtgcaggcc cccaacaggt
     3121 tgaccacgtt caggtggggc ccaaggtgac tcatgatctt cagctccgac ataagggctt
     3181 gcttctcact gctgcgggct gtggatttaa gcatcttgac ggccactttc atcgtggcct
     3241 gagaatggct caggccatga gccgtggcct ccaccacctg cccaaaggcc ccagagccga
     3301 gggtgcgtcc cagcacaagc tggtcccgcg gcagctccca cgtggagtca tagggcagct
     3361 gcatggggtc cacgtagatg tactcatggc cgtcagagct cacagactca atcaccttcc
     3421 atcggatctc gtaacgtggc ttcttctgcc aaagcatgat gaggatgata agggagatga
     3481 tggtgagcac caccagggcc aggatggctg agatcaccac caccttaaag ggcaaggctt
     3541 gcaggctgtt cccgttggtg ctggtgcagg tgggaggtgg ggaggtctgg ggtctgacga
     3601 ctggtgcgaa agcactcttc tgtttcacgg ctgagaccat gttggctggt gagaaggaga
     3661 agatgcccga ggagctgctg cagttggagg ggaggcttgt ggaggaggcc atggtggggc
     3721 tggatggcac tatggcatag ggggagttgg cagctgagcc gttgaggaag gtgggggagc
     3781 cgcccaaatt ggacattgcg gcagagccgt atccgttcat gctcgtggtg acggagttat
     3841 agttggtctg ctggggagtg gtgctcggca cgtacccgtg tggtgatacg ctgcttgagt
     3901 tgcgggtgaa accctgattg gtggcttgtg atgcctcgga gacattcacg gccagttgtc
     3961 cactgaacga attcacgccc atcatccctg cgtggaccga ggtgttagca agggccggga
     4021 gttggttgtg gttgcgggga acactgtaca gggcctcggc aatgtcggct gctctcttca
     4081 gaatgatttc ctggttgttg tgtggcatcc catacagtgc ttctaccaga tccgcagccc
     4141 ttttgagtat tacttccttt ggcaaacgct cagggtcacc agggtgccga ggaatgacct
     4201 tctgtaacct ctggaaacca taatcgatgg tgggttcgtt gagcgctgtg taaatgaatc
     4261 tgcctggtgt tcctttgcag aactgcttag atttgtagga cagtgtgact tccacaacac
     4321 cagggatgtg ccgaggaggg gtctgcacac ggatggcatg aggagtgatc aactcactcc
     4381 agaccagcat ggtaccgaat atgacctgta acccatcaaa gaaattgtcc cctatgatga
     4441 tcacagtcgc acctcccgtc gtccatcctt cactcgggct gatggctttg atacagggag
     4501 tagcatgttc cagataagag ggcgtacctt ccgaggggtc aagcctccga gccctccgcc
     4561 catgcttgga attattatgg acaaacatgt tatcagagac tgccaggaca tggccatcca
     4621 cattgactgt cgtagacacc acgacctgga atctccgcat gtcacgtggg tttcccgcat
     4681 tctttaggca attttggtta catttgagga aaaatttcaa gaagaacctg tcaattatca
     4741 ctggatctga gggagtctca tttcggttgc cacagctttt cttgtcacaa cagcggctgc
     4801 acatgatctc atgtgtgagc aagactcggc acatttctgg gttcttgtct tggccttcat
     4861 acactatggc ttgttttgtc atggagtcaa tgaggcgcac gtagaaatcc tgctccgtcc
     4921 ttatcccatt gctgtagaga agctgaagcc ggtagtgaat tccgttattg gtcttttcgc
     4981 tgttggcttc tttttccttc tccacgaacc ccacaaacgc tgtcctctcg atctccacgg
     5041 gctggccctg tctgtcgtag agggccagga cgaagtggaa gaagttggat ttccgcagat
     5101 tggaaggcgg ctgcttctca aagtgagccc gggccagacc caccccgctc tgcgccgccg
     5161 tgttggcgtc cagcaccccg gcgccctgca tccacgtccg caccgcgttc atgccgctgc
     5221 ccagcggctc ttccttcatg ctgcttccac tccgttggat gctttcctga atcccaaaca
     5281 ttccccggga agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt
     5341 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     5401 tctcgttggg gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca
     5461 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     5521 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     5581 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     5641 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     5701 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     5761 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     5821 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     5881 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     5941 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     6001 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
     6061 cccgggcccg cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta
     6121 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     6181 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     6241 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     6301 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     6361 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     6421 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     6481 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     6541 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     6601 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     6661 atgaccccgt aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc
     6721 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     6781 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     6841 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     6901 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     6961 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     7021 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     7081 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     7141 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     7201 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     7261 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     7321 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     7381 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     7441 tttatataat tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa
     7501 cttttccatg ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg
     7561 ttttccataa tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt
     7621 atcttgtatt actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag
     7681 tggatttctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     7741 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     7801 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt
     7861 cttaagatgt tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat
     7921 agattctact actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc
     7981 tgcttgctgt gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     8041 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     8101 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     8161 tctacagcct tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct
     8221 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     8281 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     8341 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     8401 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     8461 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     8521 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     8581 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     8641 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     8701 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata
     8761 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     8821 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     8881 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     8941 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     9001 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     9061 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     9121 aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9181 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9241 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9301 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9361 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9421 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9481 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9541 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9601 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9661 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9721 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9781 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9841 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9901 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9961 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10021 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10081 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10141 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10201 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10561 taccgg
//
