LOCUS       sequence-418385-        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, NEMF_CDKL1 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_NEMF_CDKL1
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4040..4750,4751..4753,4754..4756))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4082..4103)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4431..4450
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4690..4711
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4753..4762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4838..5041)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4867..4887)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5042..5345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5602..5646)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5831..6064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6424..6541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7033..7158
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7205..7462
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7464..7843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7874..7978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7979..8047,8048..8839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8197..8216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9010..9279,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaaa
     1801 tatttggaaa gcgatagtta agttttttag tgtcgcagta gtactttttg ttgtcgagag
     1861 ccggaagtat gctacttcca gtgagttgag ggaggtactg aagtttgcta gtctcagtaa
     1921 agcaatggtg cttgcgagac ttcctaagcg tctttctggt aggcttatta tgctcttttg
     1981 ccagatcttc tatttctctt atgttttcaa agtatgggtg atgaaggagt tgttcgcaag
     2041 tcagtcgctg tgtggggtcc atgtgcaggc agcctttaag cagtccaagg gctggatagc
     2101 tgatgttggg gaacttgagt tcgagaggtt ccatatcctc tggatctggt attttgaccc
     2161 cggaaaaata ttggttagtg ctaaatacct gttgatgcct tgggatcaag tcgccgagag
     2221 ttttgcgtat caagtagagt tgatctacgt cgctttttcc gggccacagg ggaactccag
     2281 acagcagctc agcgaagacg caaccaatag cccatacatc taccggggga ccatactgag
     2341 tgtctcctac aagcaattcg ggagaccgat accacctggt ggcaacataa tccgtgtaat
     2401 aatctgaggg cccggtaaga aggcgtgcga acccgaaatc gcagagtttt attacggagt
     2461 gcttcgttat caagatgttt tctggtttga catcccgatg aatgcatttt tctgccgctt
     2521 cgacggtctt ctgggtcttc ttcgctgcgt atcgcttgtg atcataatac ttcttggcgt
     2581 ttgcgtacgc actcaatgag aggtctacgt ctaccaagag tggcttattt ttctgcggct
     2641 tctggagttg cttgttcttc tgttttttct ttttaccctt gggtggctcg gtctcgttct
     2701 tctcaacgtt cacatcgcca tctacatcat cgtcctcttc ctcggagagc aaataggggt
     2761 ttcgcaacag catggtgaca tggtttgtct gcaacttcag ttccttaatt gcgctagcga
     2821 cagggtcccc ttgcgcttgg gcttccttca caataagtcc aatttccgtc caatcaatct
     2881 ggttagcaag agcagacctt accacctgaa tagctctatc gactatttgg aggttcattt
     2941 caatcagctc tcccttcagc ttgtctattt cctgggcctg ctgaagggct tcaagtctgt
     3001 tctcatgatc tttccgtacg ttatccaatt tcttgagcgc ttgcttctcc tgttgcagtg
     3061 ctttcagatc gatcttttga ccttcgatct tggaatagaa ctcgtcaaca gctttatcaa
     3121 aactctcaaa ttcaatatac gggcactggc tgtgctgaga aaacaagaaa gggtgaaact
     3181 cctcatacgt aagaatgtct tcaaccggct tgtccgcttc aagggaaggt ttgatctctc
     3241 tcttctgtat gatgtaacct tttccgctaa aattagaagt agtcttcata taatcctccg
     3301 ctttttgcaa tgagaccaac accttttcta tgtccttcgt ctccagtttc tcgtctactt
     3361 ttacattccc agaaaacccg ttttccagca ggcagtgctc gatcaaagcg ggaccatacg
     3421 gcaacaaggg attgagtact ctcttgagga gctccccctt aggggcggaa gcaacaatct
     3481 cagtcaatcg ttcgagcgtc agaagtggtt cggcggccct cgcgtggtcc agcgggtacc
     3541 gttctcggac ggcgaatttt acatcatcgg cttcgtcggt tcgaaaccgc aagatattga
     3601 gaataacgta ttcgtagtct gtaagtacta tattgccgcg atcatagagc tctattatca
     3661 ggtgataagc tgcctcgtca gacccaaatt gaaaatccac tattctgtcc actcccaact
     3721 gtttggcgct cacaagcctc cgagacttca aatgtttgcg acacttcatc gcgaagctgc
     3781 taggcatcat gttcttaggc cactcgaact ccgtagtgtg aatgcggatg ccggattcca
     3841 acaaaagagt cgccttaaaa tctggtttct gaagtcgtat cagatatgtc ttgttgtcta
     3901 catcgtagac gttgttaacc cgcattccaa gcagactagc gttcagctca gccaagacag
     3961 ctctgaggtc aattgtgctg aatcgggact tcattccccg ggaagcggcc gcacttcccc
     4021 cggagcctcc gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     4081 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
     4141 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     4201 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     4261 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     4321 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     4381 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     4441 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     4501 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     4561 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     4621 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     4681 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     4741 cgcccttgct caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
     4801 gaattcggtt cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc
     4861 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     4921 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     4981 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     5041 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     5101 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     5161 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     5221 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     5281 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     5341 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     5401 aactagcacg cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa
     5461 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     5521 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     5581 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     5641 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     5701 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     5761 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     5821 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     5881 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     5941 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     6001 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     6061 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     6121 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     6181 cctcatatct cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg
     6241 tctacttgcc acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc
     6301 ttttcttctt ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc
     6361 tttccagagg agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac
     6421 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     6481 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     6541 attgtggatg aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac
     6601 ctgtcctata attttcttta attctttatt catagattct actactcctt gactttgggg
     6661 attgtaggga atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt
     6721 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     6781 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     6841 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     6901 gccagtatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     6961 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     7021 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     7081 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     7141 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     7201 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     7261 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     7321 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     7381 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     7441 agtcccgccc aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc
     7501 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     7561 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     7621 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     7681 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     7741 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     7801 cccgtaattg attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt
     7861 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7981 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8041 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8101 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8161 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8221 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8281 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8341 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8401 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8461 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8521 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8581 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8641 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8701 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8761 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8821 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9241 agtggctgct gccagtggcg ataagtcgtg tcttaccgg
//
