LOCUS       sequence-418434-        4782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 promoter 1-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_Promoter_1-3
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    218..266
                     /note="synthetic polyadenylation signal"
     misc_feature    280..371
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     320..339
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        947..978
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1005..1014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1011..2663
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2707..2828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2718..2737)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2772..2791
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3076..3093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3247..3835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3327..3346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4035..4782,1..44,45..113))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa aacaggcaag
       61 caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct catactcgtc
      121 ctttttcaat attattgaag catttatcag ggttactagt acgtctctca aggataagta
      181 agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt tattttcatt
      241 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa
      301 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag
      361 aacatttctc tggcctaact ggccggtacc gcggacccgc ggcagtgcct tatctctgcg
      421 gcgcacacgg ccccggcgcg cctcggccca tgctaacgag gcctggaacg tgagtgggat
      481 tacagcgcgt cggtggaggg gccgggcggg cgcggggcgg gcgggggagc gggggccgcg
      541 ggaggggccg ggccgccgcc gctcaggacc gggcctcaaa atggccacac gcgtaccccc
      601 gtagcggaaa aacgtgagca ttctggcctt tttctagggg aaaagcaacc cgcggcctcc
      661 caccgaattc ttcatcaatt tcctcctctt tttccccacg tccgcaaaag aggtcttcgc
      721 tccctttccc tcggttcctt tcgatggccg gggggggcgg tgggggggca ttttccacgg
      781 acgcccccgc cccggctgcc gccgcccctc ccgcctcgga gactctcttc cttccttccc
      841 ccttttcctt acgcaatata cagaaatgcg cgaggctgtg gttggttttc gagctcgcta
      901 gcctcgagga tatcaagatc tggcctcggc ggccaagctt agacactaga gggtatataa
      961 tggaagctcg acttccagct tggcaatccg gtactgttgg taaagccacc atggaagatg
     1021 ccaaaaacat taagaagggc ccagcgccat tctacccact cgaagacggg accgccggcg
     1081 agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcaccatc gcctttaccg
     1141 acgcacatat cgaggtggac attacctacg ccgagtactt cgagatgagc gttcggctgg
     1201 cagaagctat gaagcgctat gggctgaata caaaccatcg gatcgtggtg tgcagcgaga
     1261 atagcttgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc
     1321 cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc agccagccca
     1381 ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa aagaagctac
     1441 cgatcataca aaagatcatc atcatggata gcaagaccga ctaccagggc ttccaaagca
     1501 tgtacacctt cgtgacttcc catttgccac ccggcttcaa cgagtacgac ttcgtgcccg
     1561 agagcttcga ccgggacaaa accatcgccc tgatcatgaa cagtagtggc agtaccggat
     1621 tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg
     1681 accccatctt cggcaaccag atcatccccg acaccgctat cctcagcgtg gtgccatttc
     1741 accacggctt cggcatgttc accacgctgg gctacttgat ctgcggcttt cgggtcgtgc
     1801 tcatgtaccg cttcgaggag gagctattct tgcgcagctt gcaagactat aagattcaat
     1861 ctgccctgct ggtgcccaca ctatttagct tcttcgctaa gagcactctc atcgacaagt
     1921 acgacctaag caacttgcac gagatcgcca gcggcggggc gccgctcagc aaggaggtag
     1981 gtgaggccgt ggccaaacgc ttccacctac caggcatccg ccagggctac ggcctgacag
     2041 aaacaaccag cgccattctg atcacccccg aaggggacga caagcctggc gcagtaggca
     2101 aggtggtgcc cttcttcgag gctaaggtgg tggacttgga caccggtaag acactgggtg
     2161 tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat catgagcggc tacgttaaca
     2221 accccgaggc tacaaacgct ctcatcgaca aggacggctg gctgcacagc ggcgacatcg
     2281 cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc ctgatcaaat
     2341 acaagggcta ccaggtagcc ccagccgaac tggagagcat cctgctgcaa caccccaaca
     2401 tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg cccgccgcag
     2461 tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga gatcgtggac tatgtggcca
     2521 gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta
     2581 aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt aaggccaaga
     2641 agggcggcaa gatcgccgtg taataattct agagtcgggg cggccggccg cttcgagcag
     2701 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     2761 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     2821 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     2881 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
     2941 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     3001 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     3061 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3661 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3901 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3961 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
     4021 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
     4081 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
     4141 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
     4201 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
     4261 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
     4321 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
     4381 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
     4441 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
     4501 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
     4561 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
     4621 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
     4681 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
     4741 ccgctattga gatccagttc gatatagccc actcttgcac cc
//
