LOCUS       sequence-418439-       11015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing a dead GFP adenine base editing 
            reporter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Zhang H, Kelly K, Lee J, Echeverria D, Cooper D, Panwala R, Amrani 
            N, Chen Z, Gaston N, Wagh A, Newby GA, Xie J, Liu DR, Gao G, Wolfe 
            SA, Khvorova A, Watts JK, Sontheimer EJ
  TITLE     Self-delivering, chemically modified CRISPR RNAs for AAV co-delivery
            and genome editing in vivo.
  JOURNAL   Nucleic Acids Res. 2023 Nov 30:gkad1125. doi: 10.1093/nar/gkad1125.
  PUBMED    38033325
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Nov 30:gkad1125. doi: 10.1093/nar/gkad1125."
COMMENT     Sequence Label: pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR
FEATURES             Location/Qualifiers
     source          1..11015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1780..2159
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2160..2363
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2405..2424
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2405..2423
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2431..2440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2437..2439,2440..2442,2443..3156)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMK*HDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIHHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2482..2503)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2743..2762)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3090..3111
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3163..3180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3209..3433
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3504..3520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3528..3544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3552..3558,3559..3576,3577..3582))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3597..3618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3735..3856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4014..4412)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(4431..4478)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4526..4855)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4540..4675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    4894..5482
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4947..4967)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5365..5376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5485..5501)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6007..6187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6219..6443
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6489..6917
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6576..6595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6786..6807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      7091..7225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7287..7334
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7353..7727
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7857..7978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8027..8043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8051..8067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8075..8081,8082..8099,8100..8105))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8120..8141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8258..8275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8429..9017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8509..8528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9188..9979,9980..10048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9811..9830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10049..10153)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10228..10247)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10419..10798
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10800..10998
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattaggct agccgcgttg acattgatta ttgactagtt
     1801 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1861 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1921 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1981 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2041 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2101 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2161 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     2221 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2281 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     2341 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg
     2401 ccgctaatac gactcactat agggagagcc gccaccatgg tgagcaaggg cgaggagctg
     2461 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     2521 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2581 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2641 gtgcagtgct tcagccgcta ccccgaccac atgaagtagc acgacttctt caagtccgcc
     2701 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2761 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2821 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2881 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2941 caccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3001 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3061 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3121 gggatcactc tcggcatgga cgagctgtac aagtaaaccg gtcatcatca ccatcaccat
     3181 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3241 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3301 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3361 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3421 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct
     3481 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3541 acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga
     3601 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3661 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3721 cgctacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3781 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3841 ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga
     3901 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     3961 tggaatcgaa atctcgtagc acgtgtcagt cctgctcctc ggccacgaag tgcttagccc
     4021 tcccacacat aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca
     4081 ctgtccttca ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt
     4141 ccgcaggggc tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg
     4201 ccagctgccg cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta
     4261 aaatgatata cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg
     4321 acgctgtagt cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg
     4381 gtggattctt cttgagacaa aggcttggcc atggtttagt tcctcacctt gtcgtattat
     4441 actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa
     4501 tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     4561 actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa
     4621 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     4681 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4741 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4801 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4861 acagaattca aattcgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt
     4921 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4981 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5041 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5101 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5161 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5221 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5281 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     5341 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5401 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5461 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     5521 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     5581 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5641 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5701 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5761 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     5821 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     5881 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     5941 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     6001 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6061 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6121 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6181 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6241 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6301 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6361 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6421 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6481 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6901 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7081 gcaaccatag tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7141 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7201 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7261 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     7321 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     7381 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     7441 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     7501 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     7561 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     7621 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     7681 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     7741 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7801 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7861 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7921 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7981 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8041 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8101 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8161 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8221 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8281 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8341 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8401 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8461 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8521 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8581 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8641 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8701 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8761 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8821 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8881 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8941 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9001 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9061 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9181 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9241 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9301 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9361 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9421 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9481 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9541 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9601 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9661 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9721 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9781 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9841 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9901 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9961 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10021 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10081 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10141 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10201 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10261 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    10321 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
    10381 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
    10441 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
    10501 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
    10561 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
    10621 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
    10681 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
    10741 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
    10801 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
    10861 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
    10921 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
    10981 ggaggtctat ataagcagcg cgttttgcct gtact
//
