LOCUS       sequence-418461-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human CXCR4-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(CXCR4-E2(37))-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        258..456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     411..431
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             474..654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    701..826
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1325..1558
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1743..1787
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2085..2202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2277..2525
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2277..2297
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2448..2467
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2654..3153
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2689..2712)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3086..3105
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3193..3789
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3193..3212)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3689..3709
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3808..3861
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3862..4563
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4623..4643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5041..5052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5504..5521)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5510..5734
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5760..5780)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5760..5778)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5799..5815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5799..5815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5812..5834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5823..5839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5847..5853,5854..5871,5872..5877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5892..5913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6030..6047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6201..6789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6281..6300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6960..7751,7752..7820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7583..7602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7821..7925)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7951..8406)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8088..8109)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8300..8319
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8533..8555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8547..8564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8548..8564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8574..8593
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8574..8592
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
       61 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      121 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      181 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      241 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      301 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      361 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      421 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      481 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      541 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      601 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      661 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      721 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      781 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      841 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      901 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
      961 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1021 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1081 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1141 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1201 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1261 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1321 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1381 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1441 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1501 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1561 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1621 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1681 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1741 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1801 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1861 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1921 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1981 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2041 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2101 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2161 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2221 agcagagatc cagtttggtt agtaccgggc cctacgcgtc caaggtcggg caggaagagg
     2281 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     2341 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     2401 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     2461 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2521 acaccgagtc aaaacttcaa agttggttta agagctatgc tggaaacagc atagcaagtt
     2581 taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttgg
     2641 atccaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     2701 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     2761 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     2821 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     2881 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     2941 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     3001 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     3061 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     3121 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgagcag
     3181 ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3241 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3301 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3361 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     3421 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3481 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3541 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3601 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3661 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3721 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3781 cccggtgccg gcggcgggtc cggaggagag ggcagaggaa gtctcctaac atgcggtgac
     3841 gtggaggaga atcctggccc aatgagcgag ctgattaagg agaacatgca catgaagctg
     3901 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     3961 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     4021 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     4081 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     4141 accacatacg aggacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     4201 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     4261 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     4321 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     4381 atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     4441 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     4501 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     4561 tgagcggccg ctcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4621 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4681 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4741 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4801 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4861 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4921 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4981 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5041 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5101 ccgcgtctac gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcag
     5161 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5221 aaggggggac tggaagggct aattcactcc caaagaagtc aagatctgct ttttgcctgt
     5281 actgggtctc tctggttaga ccagagtctc tctggttaga ccagatctga gcctgggagc
     5341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5461 agtcagtgtg gaaaatctct agcagtttaa acccgctgat cagcctcgac tgtgccttct
     5521 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5581 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5641 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagtcaat
     5701 agcaggcatg ctggggatgc ggtgggctct atgggcggcc gttaattaag cttttgttcc
     5761 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     5821 aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa gtgtaaagcc
     5881 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5941 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6001 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6061 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7921 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     7981 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     8041 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     8101 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     8161 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     8221 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     8281 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     8341 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     8401 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8461 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8521 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     8581 actcactata gggcgaattg actagtacat aacttacggt aaatggcccg cctggctgac
     8641 cgcccaacga
//
