LOCUS       sequence-418462-        5906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Cre recombinase from the broadly active CAG 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCAG-Cre
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        582..961
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        992..1096
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1097..1165,1166..1957)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1315..1334)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2128..2716
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2617..2636
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2870..2887
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2955..3089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2979..2998)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3033..3052
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(3095..3290)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3109..3244
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    3349..3370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3385..3390,3391..3408,3409..3415)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3423..3439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3428..3450
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3447..3463
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3447..3463
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3806..3825
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3824..3879
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3925..3944
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4035..4064)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4074..5102)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     regulatory      5099..5108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5142..5161)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          join(5169..5906,1..279)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5223..5245)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc ccgagccgct
       61 ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc acagaaaaga
      121 aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc
      181 gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcggc
      241 ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt
      301 tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga gcgcgccgct
      361 ctgattggct gccgccgcac ctctccgcct cgccccgccc cccccccccc cccccccccc
      421 cccccccccc cccccccccc cccccccctc gcccaccccc cccccccccc ccccgccccc
      481 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
      541 gagatgggga gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact
      601 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      661 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
      721 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
      781 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
      841 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
      901 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
      961 cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1021 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1081 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1141 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1201 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1261 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1321 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1381 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1441 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1501 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1561 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1621 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1681 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1741 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1801 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1861 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1921 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1981 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2041 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2101 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2161 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2221 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2281 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2341 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2401 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2461 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2521 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2581 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2641 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2701 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2761 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2821 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2881 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2941 tcattaatgc agcggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     3001 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3061 taaccattat aagctgcaat aaacaagtta gctttttgca aaagcctagg cctccaaaaa
     3121 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     3181 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     3241 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc ggatccgctg
     3301 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     3361 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     3421 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     3481 tgggctgcag gtcgagggat ctccataaga gaagagggac agctatgact gggagtagtc
     3541 aggagaggag gaaaaatctg gctagtaaaa catgtaagga aaattttagg gatgttaaag
     3601 aaaaaaataa cacaaaacaa aatataaaaa aaatctaacc tcaagtcaag gcttttctat
     3661 ggaataagga atggacagca gggggctgtt tcatatactg atgacctctt tatagccaac
     3721 ctttgttcat ggcagccagc atatgggcat atgttgccaa actctaaacc aaatactcat
     3781 tctgatgttt taaatgattt gccctcccat atgtccttcc gagtgagaga cacaaaaaat
     3841 tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa
     3901 ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt
     3961 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
     4021 tgcggccgct atcacagatc ttcttcagaa ataagttttt gttccaccgg tccatcgcca
     4081 tcttccagca ggcgcaccat tgcccctgtt tcactatcca ggttacggat atagttcatg
     4141 acaatattta cattggtcca gccaccagct tgcatgatct ccggtattga aactccagcg
     4201 cgggccatat ctcgcgcggc tccgacacgg gcactgtgtc cagaccaggc caggtatctc
     4261 tgaccagagt catccttagc gccgtaaatc aatcgatgag ttgcttcaaa aatcccttcc
     4321 agggcgcgag ttgatagctg gctggtggca gatggcgcgg caacaccatt ttttctgacc
     4381 cggcaaaaca ggtagttatt cggatcatca gctacaccag agacggaaat ccatcgctcg
     4441 accagtttag ttacccccag gctaagtgcc ttctctacac ctgcggtgct aaccagcgtt
     4501 ttcgttctgc caatatggat taacattctc ccaccgtcag tacgtgagat atctttaacc
     4561 ctgatcctgg caatttcggc tatacgtaac agggtgttat aagcaatccc cagaaatgcc
     4621 agattacgta tatcctggca gcgatcgcta ttttccatga gtgaacgaac ctggtcgaaa
     4681 tcagtgcgtt cgaacgctag agcctgtttt gcacgttcac cggcatcaac gttttctttt
     4741 cggatccgcc gcataaccag tgaaacagca ttgctgtcac ttggtcgtgg cagcccggac
     4801 cgacgatgaa gcatgtttag ctggcccaaa tgttgctgga tagtttttac tgccagaccg
     4861 cgcgcctgaa gatatagaag ataatcgcga acatcttcag gttctgcggg aaaccatttc
     4921 cggttattca acttgcacca tgccgcccac gaccggcaaa cggacagaag cattttccag
     4981 gtatgctcag aaaacgcctg gcgatccctg aacatgtcca tcaggttctt gcgaacctca
     5041 tcactcgttg catcgaccgg taatgcaggc aaattttggt gtacggtcag taaattggcc
     5101 atggtggcgg ctcagaattc tttgccaaaa tgatgagaca gcacaataac cagcacgttg
     5161 cccaggagct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc tctagagccg
     5221 ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc
     5281 ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg cgacgcacga
     5341 aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta
     5401 gagggggtgc ggcggcgcct cccagatttc ggctccgcac agatttggga caaaggaagt
     5461 ccctgcgccc tctcgcacga ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga
     5521 cagccgccgg cgctccgggg gccgccgcgc ccctcccccg agccctcccc ggcccgaggc
     5581 ggccccgccc cgcccggcac ccccacctgc cgccaccccc cgcccggcac ggcgagcccc
     5641 gcgccacgcc ccgtacggag ccccgcaccc gaagccgggc cgtgctcagc aactcgggga
     5701 ggggggtgca gggggggttg cagcccgacc gacgcgccca caccccctgc tcaccccccc
     5761 acgcacacac cccgcacgca gcctttgttc ccctcgcagc cccccccgca ccgcggggca
     5821 ccgcccccgg ccgcgctccc ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc
     5881 ccgcagcgct cacagccgcc gggcag
//
