LOCUS       sequence-418473-        5983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Castoreno AB, Wang Y, Stockinger W, Jarzylo LA, Du H, Pagnon JC, 
            Shieh EC, Nohturfft A
  TITLE     Transcriptional regulation of phagocytosis-induced membrane 
            biogenesis by sterol regulatory element binding proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34.
  PUBMED    16141315
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13129-34."
COMMENT     Sequence Label: pHMGCS-Luc (aka: pES8)
FEATURES             Location/Qualifiers
     source          1..5983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    27..161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(303..320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(474..1062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(554..573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1233..2024,2025..2093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1856..1875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2094..2198)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2225..2680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2312..2331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2522..2543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2870..2991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3023..3034)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3477..5129
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3543..3561)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          5370..5435
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5565..5585
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
       61 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      121 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtcgaccg atgcccttga
      181 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
      241 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
      301 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      361 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2221 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2281 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2341 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2401 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2461 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2521 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2581 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2641 tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg
     2701 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
     2761 ccatgataag taagtaatat taaggtacgt ggaggtttta cttgctttaa aaaacctccc
     2821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atggtactgt
     3001 aactgagcta acataaccca attcgccctt cgatctcctc tcactgacct tcaattggtc
     3061 agagaacctc tcactccgct cccgttggct ctcgccgtct ctcgccgtcc cgccattggc
     3121 cgagatgttc tcgggccacc ctacgtcacc gtctcacccc acttccccgg tcctccgact
     3181 ttgtccccgc cccttccccc acccttccag ccgcgaggga aaaccctagc gagtcaccgc
     3241 ctctcctttc cttcccattg gcagaaagcg gtatgagggg cgggagctga cgagcgagtc
     3301 tgagctctgg gattggctgc ggcagccgcc atcccgcccc ctgccgcctc ccggcggtgc
     3361 gggagtcggg tgggtcggtg gctataaagc tgcaagggcg aattgggagg taccgagctc
     3421 ttacgcgtgc tagctcgaga tctaagtaag cttggcattc cggtactgtt ggtaaaatgg
     3481 aagacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     3541 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     3601 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc
     3661 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     3721 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     3781 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc
     3841 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     3901 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc
     3961 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     4021 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta
     4081 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg
     4141 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     4201 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     4261 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa
     4321 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg
     4381 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag
     4441 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc
     4501 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     4561 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     4621 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg
     4681 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     4741 acatagctta ctgggacgaa gacgaacact tcttcatagt tgaccgcttg aagtctttaa
     4801 ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc
     4861 ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt gaacttcccg
     4921 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     4981 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     5041 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     5101 ccaagaaggg cggaaagtcc aaattgtaaa atgtaactgt attcagcgat gacgaaattc
     5161 ttagctattg taatactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
     5221 ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
     5281 cagaaattcg ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     5341 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     5401 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     5461 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     5521 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     5581 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     5641 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     5701 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     5761 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     5821 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     5881 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     5941 cttgctttaa aaaacctccc acacctcccc ctgaacctga aac
//
