LOCUS       sequence-418476-       19292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PE2 for prime editing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19292)
  AUTHORS   Zhong Z, Fan T, He Y, Liu S, Zheng X, Xu Y, Ren J, Yuan H, Xu Z, 
            Zhang Y
  TITLE     An improved plant prime editor for efficient generation of 
            multiple-nucleotide variations and structural variations in rice.
  JOURNAL   Plant Commun. 2024 May 14:100976. doi: 10.1016/j.xplc.2024.100976.
  PUBMED    38751122
REFERENCE   2  (bases 1 to 19292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2024 May 14:100976. doi: 10.1016/j.xplc.2024.100976."
COMMENT     Sequence Label: pGEL682
FEATURES             Location/Qualifiers
     source          1..19292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             join(2017..4533,4534..4536,4537..6120)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             6033..6041
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6121..6168
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8341..8361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8370..8618
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8880..9185)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8982..9001)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9516..9540)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9570..9594
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10895..11524
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11953..13026
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13092..13286
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13522..13544)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13620..13760
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13675..13694
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13775..13792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13946..14534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14026..14045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15959..16753)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    17178..17202
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    17280..17454
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(18544..19220)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(18587..18607)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggccatatgg acaagaagta ctcgatcggc
     2041 ctcgatattg ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc
     2101 tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt
     2161 ggcgctctcc tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc
     2221 cgcaggcggt acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac
     2281 gagatggcga aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag
     2341 gaggataaga agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac
     2401 cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag
     2461 gcggacctcc gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc
     2521 ctgatcgagg gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc
     2581 gtgcagacgt acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg
     2641 aaggctatcc tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag
     2701 ctgccgggcg agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc
     2761 acgcccaact tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag
     2821 gacacatacg acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac
     2881 ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg
     2941 aacaccgaga ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac
     3001 catcaggatc tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag
     3061 gagatcttct tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct
     3121 caggaggagt tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag
     3181 ctgctggtga agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc
     3241 agcatcccac accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac
     3301 ttctaccctt tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc
     3361 ccgtactacg tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag
     3421 tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct
     3481 cagtcgttca tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc
     3541 cctaagcact cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag
     3601 tatgtgaccg agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc
     3661 gtggacctcc tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac
     3721 ttcaagaaga ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac
     3781 gcctccctgg ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac
     3841 aacgaggaga atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac
     3901 cgggagatga tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg
     3961 aagcagctca agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac
     4021 ggcattcggg acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc
     4081 gcgaaccgca atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc
     4141 cagaaggctc aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct
     4201 ggctcgccag ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg
     4261 aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag
     4321 accacgcaga agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt
     4381 aaggagctgg ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat
     4441 gagaagctct acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg
     4501 gatattaaca ggctcagcga ttacgacgtc gatgctatcg ttccacagtc attcctgaag
     4561 gatgactcca ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat
     4621 aatgttcctt cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat
     4681 gccaagctga tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc
     4741 tctgagctgg acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact
     4801 aagcacgttg cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag
     4861 ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag
     4921 gatttccagt tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac
     4981 ctgaacgcgg tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc
     5041 gtgtacgggg actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag
     5101 attggcaagg ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca
     5161 gagatcactc tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag
     5221 acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc
     5281 atgccacaag tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag
     5341 tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg
     5401 aagaagtacg gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag
     5461 gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt
     5521 atggagcgct ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag
     5581 gaggtgaaga aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac
     5641 ggcaggaagc ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg
     5701 ccgtccaagt atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc
     5761 cccgaggaca acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag
     5821 atcattgagc agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat
     5881 aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat
     5941 atcattcatc tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac
     6001 acaactatcg atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc
     6061 caccagtcga ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac
     6121 aagcggccag cggcgacgaa gaaggcgggg caggcgaaga agaagaagtc agggggttct
     6181 agcggcggtt catctggctc cgaaacaccg ggaacttccg agagtgctac tccagaatct
     6241 agcggtgggt catctggagg gtcttccaca cttaatattg aggacgaata ccgcctgcat
     6301 gaaactagta aagaacccga cgtgagcctc ggtagcacgt ggctctctga tttcccgcaa
     6361 gcatgggctg aaacaggtgg gatgggcctc gctgttcgcc aggctccatt gatcatccca
     6421 ctcaaggcca cttctacacc ggtgtccata aagcaatacc caatgagcca ggaagctcgg
     6481 cttggtatca agcctcacat ccaaagattg cttgatcagg gaattcttgt cccttgtcaa
     6541 agtccatgga acacccctct tcttcccgtc aaaaaacctg ggactaacga ttataggcca
     6601 gtgcaagacc tcagggaggt taacaaaagg gttgaagaca ttcatccaac agtgccgaat
     6661 ccttataatc tgctctcagg tttgcctcca agtcaccagt ggtatacagt gctggatctt
     6721 aaagacgcat ttttttgtct gcgccttcat cccacttcgc aacctctttt tgctttcgaa
     6781 tggagagatc cagagatggg tatctccgga cagctcacat ggacccggtt gccccaaggc
     6841 ttcaaaaact ccccaacctt gtttaatgaa gcgctgcaca gagatttggc ggacttcagg
     6901 atccagcacc ccgacttgat tctcctgcag tatgtggatg accttttgct tgcagccaca
     6961 tctgagcttg actgccaaca ggggacgcgc gcccttcttc agactttggg aaaccttggg
     7021 tatagagcct ctgccaagaa ggcacagatc tgcccgaagc aagttaagta tctcggttat
     7081 ttgctcaagg agggtcagag gtggctgacg gaggcgagga aagaaacggt gatgggccaa
     7141 ccaaccccaa aaacccctag gcaacttaga gagtttcttg gtaaagcggg gttttgccgg
     7201 ttgttcattc caggctttgc cgagatggcc gcaccgttgt atccattgac gaaaccgggc
     7261 acccttttta attgggggcc cgaccagcag aaggcgtatc aggagatcaa gcaagctctc
     7321 ctgaccgcac cagcactcgg acttccagat cttacgaaac cgtttgagct cttcgtggat
     7381 gagaaacagg ggtatgctaa gggagtcctc acacaaaagt tgggaccgtg gaggcggccg
     7441 gttgcctatc tttcgaaaaa acttgatccc gtggcggctg gttggcctcc ctgtctgagg
     7501 atggtggccg cgatcgcagt ccttacaaaa gatgcaggga agttgactat gggacaacca
     7561 ttggtcatac ttgccccgca cgcggttgag gccctcgtta agcagcctcc cgataggtgg
     7621 ctcagtaacg cccgcatgac tcattaccag gcactgcttc tcgacacaga cagggtgcag
     7681 tttggtcccg tcgtcgcttt gaaccccgcc accctgctgc ccctcccgga ggaaggattg
     7741 cagcacaact gtctggatat cttggccgaa gcgcatggga ctcgcccgga cttgactgac
     7801 cagccccttc ccgacgctga ccatacttgg tatacggatg gaagcagcct tctgcaggaa
     7861 gggcaacgca aggcaggggc cgcggttact actgaaactg aggtcatctg ggctaaggct
     7921 ttgccagccg gaactagtgc tcaaagagca gaattgattg ccctcacgca agctttgaaa
     7981 atggctgagg gtaagaagct taatgtgtat acagacagta ggtatgcatt tgcgacagca
     8041 catatccatg gtgagatcta ccggcgcaga ggatggctta cgagcgaggg gaaggagatt
     8101 aagaacaaag atgagatttt ggccctgctc aaagcgctct tcctcccgaa gcgccttagc
     8161 atcatccatt gcccaggcca tcagaagggt cactcggccg aagcacgggg taataggatg
     8221 gccgatcagg cagcaaggaa ggctgctatc actgagacac ccgacacctc tacccttctc
     8281 atagagaatt catcgccgag cggcggcagc aagaggactg cagacgggtc ggaattcgaa
     8341 ccgaagaaaa agagaaaggt gtgagcttat atgaagatga agatgaaata tttggtgtgt
     8401 caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat
     8461 ttgtggcttt ttctaatatt aaatgaatgt aagatcacat tataatgaat aaacaaatgt
     8521 ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg
     8581 tgctatggta tggactatgg aatatgatta aagataagag gatccgcgga tcatgaacca
     8641 acggcctggc tgtatttggt ggttgtgtag ggagatgggg agaagaaaag cccgattctc
     8701 ttcgctgtga tgggctggat gcatgcgggg gagcgggagg cccaagtacg tgcacggtga
     8761 gcggcccaca gggcgagtgt gagcgcgaga ggcgggagga acagtttagt accacattgc
     8821 ccagctaact cgaacgcgac caacttataa acccgcgcgc tgtcgcttgt gtggagacct
     8881 tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga
     8941 tcagccactt cttccccgat aacggaaacc ggcacactgg ccatatcggt ggtcatcatg
     9001 cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac
     9061 agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca
     9121 ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac
     9181 tgcatttcac cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc
     9241 gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc
     9301 ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc
     9361 aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt
     9421 ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca
     9481 aatacgcata ctgttatctg gcttggtctc tctagaccca gctttcttgt acaaagttgg
     9541 cattactgcg agctcaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     9601 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     9661 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     9721 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     9781 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     9841 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     9901 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     9961 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
    10021 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
    10081 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
    10141 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
    10201 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
    10261 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
    10321 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
    10381 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
    10441 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
    10501 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
    10561 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
    10621 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
    10681 agccgcccgc gcacggctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
    10741 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
    10801 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
    10861 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
    10921 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
    10981 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
    11041 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
    11101 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
    11161 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
    11221 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
    11281 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
    11341 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
    11401 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
    11461 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
    11521 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
    11581 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
    11641 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
    11701 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
    11761 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
    11821 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
    11881 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
    11941 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
    12001 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
    12061 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
    12121 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
    12181 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
    12241 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
    12301 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
    12361 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
    12421 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
    12481 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
    12541 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
    12601 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
    12661 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
    12721 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
    12781 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
    12841 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
    12901 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
    12961 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
    13021 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
    13081 ggcctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
    13141 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
    13201 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
    13261 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
    13321 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
    13381 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
    13441 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
    13501 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggaaacgg tcacagcttg
    13561 tctgtaagcg gatgccggga gcagacaagc gcgtcagcgg gtgttggcgg gtgtcggggc
    13621 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
    13681 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    13741 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    13801 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    13861 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    13921 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    13981 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    14041 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    14101 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    14161 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    14221 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    14281 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    14341 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    14401 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    14461 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    14521 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    14581 aaaactcacg ttaagggctg atgaatcccc taatgatttt tatcaaaatc attaagttaa
    14641 ggtagataca catcttgtca tatgatcaaa tggtttcgcc aaaaatcaat aatcagacaa
    14701 caaaatgtgc gaactcgata ttttacacga ctctctttac caattctgcc ccgaattaca
    14761 cttaaaacga ctcaacagct taacgttggc ttgccacgcc ttacttgact gtaaaactct
    14821 cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa
    14881 acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg ctgtataccg
    14941 ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg ttgactggtc
    15001 tgatatccgt gagcaaaaac ggcttatggt attgcgagct tcagtcgcac tacacggtcg
    15061 ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa agaaagctca
    15121 tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac cgctcattgt
    15181 cagtgatgct ggctttaaag tgccatggta taaatccgtt gagaagctgg gttggtactg
    15241 gttaagtcga gtaagaggaa aagtacaata tgcagaccta ggagcggaaa actggaaacc
    15301 tatcagcaac ttacatgata tgtcatctag tcactcaaag actttaggct ataagaggct
    15361 gactaaaagc aatccaatct catgccaaat tctattgtat aaatctcgct ctaaaggccg
    15421 aaaaaatcag cgctcgacac ggactcattg tcaccacccg tcacctaaaa tctactcagc
    15481 gtcggcaaag gagccatgga ttctagcaac taacttacct gttgaaattc gaacacccaa
    15541 acaacttgtt aatatctatt cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa
    15601 aagtcctgcc tacggactag gcctacgcca tagccgaacg agcagctcag agcgttttga
    15661 tatcatgctg ctaatcgccc tgatgcttca actaacatgt tggcttgcgg gcgttcatgc
    15721 tcagaaacaa ggttgggaca agcacttcca ggctaacaca gtcagaaatc gaaacgtact
    15781 ctcaacagtt cgcttaggca tggaagtttt gcggcattct ggctacacaa taacaaggga
    15841 agacttactc gtggctgcaa ccctactagc tcaaaattta ttcacacatg gttacgcttt
    15901 ggggaaatta tgaggggatc tctcagcgtt aagggatttt ggtcatgcat tctaggtact
    15961 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    16021 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    16081 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    16141 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    16201 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    16261 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    16321 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    16381 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    16441 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    16501 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    16561 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    16621 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    16681 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    16741 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    16801 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    16861 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    16921 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
    16981 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
    17041 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
    17101 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    17161 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    17221 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
    17281 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
    17341 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
    17401 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaaatagag
    17461 cttgtcgatc gttaattaat cacctccaag atcccggacg agtgctgggg cgtcggtttc
    17521 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
    17581 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
    17641 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
    17701 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
    17761 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
    17821 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
    17881 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
    17941 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
    18001 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
    18061 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
    18121 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
    18181 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
    18241 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
    18301 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
    18361 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
    18421 agagctgcat caggtcggac acgctgtcga acttttcgat cagaaacttc tcgacagacg
    18481 tcgcggtgag ttcaggcttt ttcatcctag gagagataga tttgtagaga gagactggtg
    18541 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
    18601 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
    18661 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    18721 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
    18781 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
    18841 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
    18901 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
    18961 caccatgttc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
    19021 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
    19081 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
    19141 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
    19201 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
    19261 tggagtagac gagagtgtcg tgctccacca tg
//
