LOCUS       sequence-418615-       10603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Andrews P, He YJ, Xiong Y
  TITLE     Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and 
            promotes cell growth by antagonizing p53 function.
  JOURNAL   Oncogene. 2006 Aug 3. 25(33):4534-48.
  PUBMED    16547496
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2006 Aug 3. 25(33):4534-48."
COMMENT     Sequence Label: pcDNA3-myc3-Cul7
FEATURES             Location/Qualifiers
     source          1..10603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     430..449
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    734..855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(771..790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     825..844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(904..920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(904..920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(917..939)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    928..944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(952..958,959..976,977..982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    997..1018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1135..1152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1306..1894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1386..1405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2065..2856,2857..2925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2688..2707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2926..3030)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3105..3124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3296..3675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3676..3879
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3830..3850
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3924..3943
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3924..3942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3958..3987
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3994..4023
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4030..4059
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(9217..9235)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9218..9235)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9255..9272)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9261..9485
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9531..9959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9618..9637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9828..9849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9968..9988)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9973..10302
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10153..10288
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10215..10234
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(10369..10603,1..560)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10423..10442)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
       61 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
      121 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
      181 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
      241 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
      301 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
      361 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
      421 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
      481 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
      541 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
      601 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
      661 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
      721 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      841 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
      901 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      961 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1021 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1081 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1141 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1201 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1261 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1321 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1381 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1441 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1501 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1561 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1621 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1681 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1741 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1801 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1861 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1921 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1981 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2041 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2101 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2161 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2221 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2281 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2341 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2401 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2461 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2521 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2581 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2641 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2701 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2761 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2821 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2881 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2941 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3001 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3061 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     3121 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     3181 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     3241 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     3301 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     3361 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3421 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3481 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3601 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3661 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3721 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     3781 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     3841 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     3901 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctatggag
     3961 caaaagctca tctcagagga agatctggaa ttagagcaaa agctcatctc agaggaagat
     4021 ctggaattag agcaaaagct catctcagag gaagatctgg aattcaggat cctgggagaa
     4081 ctccgctaca gggaattcag ggtgcccctg gggcccggct tacatgccta tcctgatgag
     4141 ctgatccgcc agcgcgtggg ccatgatggg catcctgagt accagatccg ttggctcatc
     4201 ctgcggcgtg gcgatgaggg ggacgggggc tctggccaag tggactgcaa ggctgagcac
     4261 atcctgctgt ggatgtccaa ggatgagatc tatgccaact gccacaagat gctgggcgag
     4321 gatggccagg tcatcgggcc ctcccaggag tctgcagggg aggttggggc cctggacaaa
     4381 tctgtgctgg aggagatgga aaccgatgtg aagtccctca ttcagagagc ccttcggcag
     4441 ctggaggagt gtgtgggcac tatccctcct gctcctctac ttcacactgt ccacgtgctc
     4501 agcgcctatg ccagcattga gcccctcact ggagtattca aggacccaag ggtcctggac
     4561 ttgctcatgc acatgttgag tagtcctgat tatcagattc gctggagtgc aggccggatg
     4621 atacaagccc tgtcctccca tgacgctggg acccggactc agatccttct gtcactgagc
     4681 caacaagaag ccattgagaa acacctggat tttgacagcc gctgtgctct gctagcactg
     4741 tttgcacagg ccacgctctc tgaacacccc atgtctttcg agggcattca gctaccacag
     4801 gtcccaggaa gggtgctctt ctccctggtg aagcggtatt tgcatgtcac ctcgctcctg
     4861 gatcagctga acgacagtgc tgcggagcca ggagcccaga acacctctgc tcctgaggag
     4921 ttgagtgggg agaggggtca actggagctg gagttcagta tggccatggg caccctgatc
     4981 tcggagctgg tgcaagccat gcgctgggac caggcctcag acagaccaag gagctcagca
     5041 cggtcccccg gttccatctt ccagcctcag ctggcagatg tgagcccagg gctccccgct
     5101 gcccaggctc agccctcctt caggaggtca agacgttttc gccctcgttc tgagttcgca
     5161 agtggcaata cctatgcttt gtatgtgcgg gacacactgc agccggggat gcgagtgcgg
     5221 atgctggatg attatgagga gatcagtgcc ggggatgagg gcgagtttcg gcagagcaac
     5281 aacggtgtgc ctcctgtgca ggtattttgg gagtcaacag gccgcaccta ttgggtgcac
     5341 tggcacatgc tggagatctt gggctttgag gaagacattg aggacatggt tgaggctgat
     5401 gagtaccaag gggcagtggc cagtagagtc ctgggtagag ccctgcctgc ctggcgctgg
     5461 aggcccatga cagaactcta tgctgtgcct tatgtgctgc ctgaggatga ggacactgag
     5521 gagtgtgaac acctgaccct ggctgagtgg tgggaactcc tcttcttcat caagaagctg
     5581 gatggacctg accatcagga ggttctccag atcctccagg agaacctaga tggggagatt
     5641 ctggatgatg agatcctagc tgaactggcc gtgcccatag aattggccca ggacttgctg
     5701 ctgactctgc cacagcgact caatgacagt gccctcaggg acctgatcaa ctgccatgtc
     5761 tacaagaagt atgggcctga agccctagca gggaaccaag cctacccatc ccttctagaa
     5821 gcccaagaag atgtcctcct gctagacgcg caggcccagg ctaaggactc agaagatgca
     5881 gccaaagtgg aagcaaaaga acccccatct cagagtccca acactcccct gcagcgtctg
     5941 gtggagggtt atggtccagc tgggaaaatc ctcctggatc tagagcaagc cctcagctca
     6001 gaggggaccc aggagaacaa ggtcaagcca ctcctgctgc agctgcagcg gcagccgcag
     6061 cccttcctgg cactgatgca gagcctggac actccggaga ctaacaggac cctgcacctg
     6121 actgtgctga gaatcctgaa gcagctggtg gacttccccg aggcactgct gctcccctgg
     6181 cacgaggccg tggatgcctg catggcctgc ctgcggtccc caaacactga tcgagaggtg
     6241 ctccaggaac tgattttctt cctgcaccgc ctgacctcag tgagcaggga ctatgccgtg
     6301 gtgctgaatc agctgggagc aagagacgct atctccaagg ccctggaaaa gcacctggga
     6361 aagctggagc tggctcagga gctgcgggac atggtgttca agtgtgagaa gcatgcccac
     6421 ctctaccgca aactcatcac caacatcctg ggaggctgca tccagatggt gctgggccag
     6481 atcgaagacc acagacgaac ccaccggccc atcaacatcc ctttctttga tgtgttcctc
     6541 agatacctgt gccagggctc cagtgtggaa gtgaaggagg acaagtgctg ggagaaggtg
     6601 gaggtgtcct ccaacccgca ccgggccagc aagctgacgg accacaaccc caagacctat
     6661 tgggagtcca acggcagcgc cggctcccac tacatcaccc tgcacatgcg ccggggcatc
     6721 ctcatcaggc aactgactct gcttgtggct agtgaggact cgagttacat gccggcccga
     6781 gtggtggtgt gcgggggtga tagcactagc tctcttcaca cggaactcaa ctcggtgaat
     6841 gtgatgccct ctgccagccg ggtgatcctc ctggagaacc tgacccgctt ctggcccatc
     6901 atccagatcc gcataaagcg ctgccagcag ggtggcattg atacgcgcat tcgggggtta
     6961 gagatcctag gccccaagcc cacgttctgg ccagtgttcc gggagcagct ctgtcgtcac
     7021 acacgcctct tctacatggt tcgggcacag gcctggagcc aggacatggc agaggaccgc
     7081 aggagcctcc tgcacctgag ttctagactc aacggtgctc tgcgccagga gcagaatttt
     7141 gctgaccgct tcctccctga tgacgaggct gcccaagctc tgggcaagac ctgctgggag
     7201 gccctggtca gccccgtggt gcagaacatc acctcccctg atgaggatgg cattagcccc
     7261 ctgggttggc tgctggacca gtacctggag tgtcaggaag ctgtcttcaa cccccagagc
     7321 cgcggcccag ctttcttctc gcgggtgcgc cgtctcactc acctgctggt gcatgtcgag
     7381 ccctgtgagg caccccctcc tgtggtggcc actcctcggc ccaaaggcag aaacagaagc
     7441 cacgactgga gctccttggc tacccggggc cttccaagca gcatcatgag aaacctgacg
     7501 cgctgttggc gggccgtggt ggagaagcag gtgaacaatt ttctgacctc atcctggcgg
     7561 gatgatgact ttgtgccacg ctactgtgag cactttaata ttctgcagaa ctcaagctct
     7621 gaactgtttg ggcctcgggc agccttcttg ctggcgctgc aaaatggctg tgcgggagcc
     7681 ttgctgaagc tcccttttct caaagctgcc cacgtgagtg agcagttcgc ccggcacatt
     7741 gaccagcaga tccagggcag ccggatcggt ggagcccagg aaatggagag gctggcacag
     7801 ctgcagcaat gcctgcaagc tgtcctgatt ttctccggct tggagatagc caccactttt
     7861 gagcattatt accagcacta catggcggac cgtctcctgg gcgtggtctc gagctggctg
     7921 gagggggccg tgctggagca gatcggtccc tgcttcccca accgcctccc ccagcagatg
     7981 ttgcagagcc tgagcacctc taaggagctg cagcgccagt tccacgtcta ccagctccag
     8041 cagctggatc aggaactcct aaagctggag gatacagaga agaaaataca ggtgggcctt
     8101 ggggccagtg gcaaggagca caagagcgag aaggaagagg aagctggggc agcagcagtg
     8161 gtggatgtgg cggagggaga ggaggaagag gaggagaatg aggacctcta ctatgaaggg
     8221 gcaatgccag aagtgtctgt gcttgtcctg tcccgacact cctggcctgt tgcctcaatc
     8281 tgccacacac tgaaccccag aacctgcctg ccctcctacc tgaggggcac tttgaacaga
     8341 tactccaact tctacaacaa gagtcagagc caccctgccc ttgagcgagg ctcacagagg
     8401 cgactgcagt ggacgtggct gggctgggct gagctgcagt ttgggaacca gaccctgcat
     8461 gtgtccaccg tgcagatgtg gctactgctg tatctcaacg acctgaaggc ggtctctgtg
     8521 gagagtctgc tggcgttctc agggctctcc gcagacatgc tcaatcaggc gattgggccc
     8581 ctcacctctt caagaggccc cctggacctt cacgagcaaa aggatatacc aggaggggtc
     8641 ctcaagattc gagatggcag caaggaaccc aggtcgagat gggacattgt gcggctcatc
     8701 ccacctcaga cgtacctgca agctgagggt gaagacggcc agaacttgga gaagagacgg
     8761 aatcttctga actgcctcat cgtccgaatc ctcaaggccc atggagatga ggggctgcac
     8821 attgaccagc ttgtctgtct ggtgctggag gcttggcaga agggcccgtg tcctcccagg
     8881 ggtttggtca gcagccttgg taaggggtct gcatgcagca gcactgacgt cctctcctgc
     8941 atcctacacc tcctgggcaa gggcacgctg agacgccatg acgaccggcc ccaggtgctg
     9001 tcctatgcag tccctgtgac tgtcatggag cctcacactg agtccctgaa cccaggctcc
     9061 tcaggcccca acccacccct caccttccat accctacaga ttcgctcccg gggtgtgccc
     9121 tatgcctcct gcactgccac ccagagcttc tctaccttcc ggtagcccta gacttggggt
     9181 caggggaagg tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag
     9241 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     9301 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     9361 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     9421 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     9481 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     9541 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9601 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9661 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9721 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9781 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9841 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9901 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     9961 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10021 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10081 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10141 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10201 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
    10261 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
    10321 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
    10381 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
    10441 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
    10501 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
    10561 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cag
//
