LOCUS       sequence-418617-        2604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for transient transfection of RDX-mCherry fusion protein in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
  TITLE     Cooperative polarization of MCAM/CD146 and ERM family proteins in 
            melanoma.
  JOURNAL   Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 
            10.1091/mbc.E23-06-0255.
  PUBMED    38117590
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255."
COMMENT     Sequence Label: RDX-mCherry
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1809..1818
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1815..2525
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1960..1978)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2228..2247
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgtcagatc cgctagcgcc accatgccga aaccaatcaa cgtaagagta actacaatgg
       61 atgctgagct ggaatttgcc attcagccca atacaactgg caaacaactt tttgaccagg
      121 tggtgaaaac agttggtttg cgtgaggtct ggttttttgg gctgcagtat gtagacagca
      181 aaggttattc tacatggctt aaactaaata aaaaggtaac acagcaggat gttaaaaaag
      241 agaatccttt acagttcaag tttagagcta aattctttcc tgaagatgtt tctgaggaat
      301 taattcaaga aataacccag agactcttct tcttgcaagt taaagaagcc atcttaaatg
      361 atgagatata ttgcccgcca gaaactgcag ttcttttggc ttcctatgct gtccaagcca
      421 agtatggaga ttacaataaa gagattcata agccaggcta cctggctaat gatagactcc
      481 taccccagcg tgtattggaa caacacaaac taacaaaaga acagtgggaa gaaagaatac
      541 agaactggca tgaagaacat agaggaatgt taagggagga ttctatgatg gaatacctga
      601 agattgcaca agatctagaa atgtatggag tcaactattt tgaaataaaa aataaaaaag
      661 gaactgaatt gtggctaggt gttgatgctt tgggtctgaa tatttatgag catgacgaca
      721 agttaacacc taaaattggt tttccctgga gtgaaatcag aaatatttca tttaatgaca
      781 aaaaatttgt tataaagcca atcgacaaaa aggcacctga ttttgtgttt tatgcacctc
      841 gtctgagaat caataagagg attttggcct tatgtatggg aaaccatgaa ctatacatgc
      901 gaagaaggaa gcctgatact attgaagtac aacagatgaa ggctcaggct agggaggaga
      961 aacatcagaa gcagttggaa agggcacaat tagagaatga aaaggagaaa agagaaatag
     1021 cagaaaagga aaaggaaaga atagaacgtg aaaaggaaga gctaatggaa cgtctaaaac
     1081 aaattgaaga gcagacaatt aaagctcaga aagaactaga agaacagact cgaaaagctc
     1141 tagaactgga tcaagaacga aaacgagcaa aagaagaagc agaacgactt gaaaaggagc
     1201 gtcgagctgc tgaagaggca aagtctgcca tagcaaaaca agctgccgac cagatgaaga
     1261 atcaggagca gctagcagca gaacttgctg aattcactgc caagattgca cttctagagg
     1321 aagccaagaa gaaaaaggaa gaggaagcta ctgagtggca acacaaagct tttgcagccc
     1381 aggaagactt ggaaaagacc aaagaagagt taaaaactgt gatgtctgcc ccccctccac
     1441 ctccaccacc accagtcatt cctccaacag aaaacgaaca tgatgaacac gatgagaata
     1501 atgctgaagc tagtgctgaa ttatcaaatg aaggggtaat gaaccataga agcgaggaag
     1561 aacgtgtaac cgaaacacag aaaaatgagc gtgttaagaa gcaacttcag gcattaagtt
     1621 cagaattagc ccaagccaga gatgaaacca agaaaacaca aaatgatgtt cttcatgctg
     1681 agaatgttaa agcaggccgt gataagtaca agactctgcg acagattcga caaggcaata
     1741 caaagcagcg tatcgatgag tttgaagcaa tggcatccgg cggtagcggc ggtggcagcg
     1801 gaccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     1861 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1921 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1981 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     2041 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     2101 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     2161 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2221 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     2281 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     2341 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2401 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2461 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2521 agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     2581 tgctttaaaa aacctcccac acct
//
