LOCUS       sequence-418619-        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for expression in mammalian cells of the proximity
            ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear 
            exclusion sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
  TITLE     Cooperative polarization of MCAM/CD146 and ERM family proteins in 
            melanoma.
  JOURNAL   Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 
            10.1091/mbc.E23-06-0255.
  PUBMED    38117590
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255."
COMMENT     Sequence Label: BirA*-HA-NES
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..48
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(174..524,525..527,528..1136)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1140..1166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1173..1199
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 cacgctaccg gtctcgagaa ttgacaagtt tgtacaaaaa agcaggctcc gctagcgcta
       61 ccggactcag atctcgagct caagcttggt accgagctcg gatcgtcgct agcgatatcg
      121 gcgcgccagc atttaaatct gtacagaccg gtgaattcgg atccggtgga ggtatgaagg
      181 acaacaccgt gcccctgaag ctgatcgccc tgctggccaa cggcgagttc cactctggcg
      241 agcagctggg agagaccctg ggaatgagca gagccgccat caacaagcac atccagacac
      301 tgagagactg gggagtggac gtgttcaccg tgcctggcaa gggctacagc ctgcctgagc
      361 ctatccagct gctgaacgcc aagcagatcc tgggacagct ggatggcgga agcgtggccg
      421 tgctgcctgt gatcgactcc accaatcagt acctgctgga cagaatcgga gagctgaagt
      481 ccggcgacgc ctgcatcgcc gagtaccagc aggctggcag aggaggcaga ggacggaagt
      541 ggttcagccc attcggagcc aacctgtacc tgtccatgtt ctggagactg gagcagggac
      601 ctgctgctgc catcggactg agtctggtga tcggaatcgt gatggccgag gtgctgagaa
      661 agctgggagc cgacaaggtg agagtgaagt ggcctaatga cctgtacctc caggaccgca
      721 agctggctgg catcctggtg gagctgacag gcaagacagg cgatgccgct cagatcgtga
      781 tcggagccgg aatcaacatg gccatgagaa gagtggagga gagcgtggtg aaccagggct
      841 ggatcaccct gcaggaggct ggcatcaacc tggaccggaa caccctggcc gccatgctga
      901 tcagagagct gagagccgct ctggagctgt tcgagcagga gggactggct ccttacctga
      961 gcagatggga gaagctggac aacttcatca acagacctgt gaagctgatc atcggcgaca
     1021 aggaaatctt cggcatctcc agaggaatcg acaagcaggg agctctgctg ctggagcagg
     1081 acggaatcat caagccctgg atgggcggag aaatctccct gagaagcgca gagaaggctt
     1141 acccttacga tgtaccggat tacgcactgc agctgcctcc cctggagcgc ctgaccctgg
     1201 actaagcggc cgctcgacgg tac
//
