LOCUS       sequence-418621-        1274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for expression in mammalian cells of the proximity
            ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane 
            localization sequence and C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
  TITLE     Cooperative polarization of MCAM/CD146 and ERM family proteins in 
            melanoma.
  JOURNAL   Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 
            10.1091/mbc.E23-06-0255.
  PUBMED    38117590
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255."
COMMENT     Sequence Label: Lyn11-BirA*-HA
FEATURES             Location/Qualifiers
     source          1..1274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..48
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(246..596,597..599,600..1208)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1212..1238
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cacgctaccg gtctcgagaa ttgacaagtt tgtacaaaaa agcaggctcc gctagcgcta
       61 ccggactcag atctcgagct caagcttggt accgagctcg gatcgtcgct agcgatatcg
      121 gcgcgccagc atttaaatct gtacagaccg gtgaattcat gggatgtata aaatcaaaag
      181 ggaaagacag cgcgggagca gatagtgctg gtagtgctgg tagtgctggt ggatccggtg
      241 gaggtatgaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc aacggcgagt
      301 tccactctgg cgagcagctg ggagagaccc tgggaatgag cagagccgcc atcaacaagc
      361 acatccagac actgagagac tggggagtgg acgtgttcac cgtgcctggc aagggctaca
      421 gcctgcctga gcctatccag ctgctgaacg ccaagcagat cctgggacag ctggatggcg
      481 gaagcgtggc cgtgctgcct gtgatcgact ccaccaatca gtacctgctg gacagaatcg
      541 gagagctgaa gtccggcgac gcctgcatcg ccgagtacca gcaggctggc agaggaggca
      601 gaggacggaa gtggttcagc ccattcggag ccaacctgta cctgtccatg ttctggagac
      661 tggagcaggg acctgctgct gccatcggac tgagtctggt gatcggaatc gtgatggccg
      721 aggtgctgag aaagctggga gccgacaagg tgagagtgaa gtggcctaat gacctgtacc
      781 tccaggaccg caagctggct ggcatcctgg tggagctgac aggcaagaca ggcgatgccg
      841 ctcagatcgt gatcggagcc ggaatcaaca tggccatgag aagagtggag gagagcgtgg
      901 tgaaccaggg ctggatcacc ctgcaggagg ctggcatcaa cctggaccgg aacaccctgg
      961 ccgccatgct gatcagagag ctgagagccg ctctggagct gttcgagcag gagggactgg
     1021 ctccttacct gagcagatgg gagaagctgg acaacttcat caacagacct gtgaagctga
     1081 tcatcggcga caaggaaatc ttcggcatct ccagaggaat cgacaagcag ggagctctgc
     1141 tgctggagca ggacggaatc atcaagccct ggatgggcgg agaaatctcc ctgagaagcg
     1201 cagagaaggc ttacccttac gatgtaccgg attacgcata ggcggccgct cgacggtacc
     1261 gcgggcccgg gatc
//
