LOCUS       sequence-418623-        2800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of MCAM-GFP fusion protein in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
  TITLE     Cooperative polarization of MCAM/CD146 and ERM family proteins in 
            melanoma.
  JOURNAL   Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 
            10.1091/mbc.E23-06-0255.
  PUBMED    38117590
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255."
COMMENT     Sequence Label: MCAM-GFP
FEATURES             Location/Qualifiers
     source          1..2800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..47
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2040..2042,2043..2045,2046..2759)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2040..2042,2043..2045,2046..2756)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2085..2106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2346..2365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2693..2714
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgctaccgg tctcgagaat tgacaagttt gtacaaaaaa gcaggctccg ctagcgctac
       61 cggactcaga tctcgagctc aagcttatgg ggcttcccag gctggtctgc gccttcttgc
      121 tcgccgcctg ctgctgctgt cctcgcgtcg cgggtgtgcc cggagaggct gagcagcctg
      181 cgcctgagct ggtggaggtg gaagtgggca gcacagccct tctgaagtgc ggcctctccc
      241 agtcccaagg caacctcagc catgtcgact ggttttctgt ccacaaggag aagcggacgc
      301 tcatcttccg tgtgcgccag ggccagggcc agagcgaacc tggggagtac gagcagcggc
      361 tcagcctcca ggacagaggg gctactctgg ccctgactca agtcaccccc caagacgagc
      421 gcatcttctt gtgccagggc aagcgccctc ggtcccagga gtaccgcatc cagctccgcg
      481 tctacaaagc tccggaggag ccaaacatcc aggtcaaccc cctgggcatc cctgtgaaca
      541 gtaaggagcc tgaggaggtc gctacctgtg tagggaggaa cgggtacccc attcctcaag
      601 tcatctggta caagaatggc cggcctctga aggaggagaa gaaccgggtc cacattcagt
      661 cgtcccagac tgtggagtcg agtggtttgt acaccttgca gagtattctg aaggcacagc
      721 tggttaaaga agacaaagat gcccagtttt actgtgagct caactaccgg ctgcccagtg
      781 ggaaccacat gaaggagtcc agggaagtca ccgtccctgt tttctacccg acagaaaaag
      841 tgtggctgga agtggagccc gtgggaatgc tgaaggaagg ggaccgcgtg gaaatcaggt
      901 gtttggctga tggcaaccct ccaccacact tcagcatcag caagcagaac cccagcacca
      961 gggaggcaga ggaagagaca accaacgaca acggggtcct ggtgctggag cctgcccgga
     1021 aggaacacag tgggcgctat gaatgtcagg gcctggactt ggacaccatg atatcgctgc
     1081 tgagtgaacc acaggaacta ctggtgaact atgtgtctga cgtccgagtg agtcccgcag
     1141 cccctgagag acaggaaggc agcagcctca ccctgacctg tgaggcagag agtagccagg
     1201 acctcgagtt ccagtggctg agagaagaga caggccaggt gctggaaagg gggcctgtgc
     1261 ttcagttgca tgacctgaaa cgggaggcag gaggcggcta tcgctgcgtg gcgtctgtgc
     1321 ccagcatacc cggcctgaac cgcacacagc tggtcaacgt ggccattttt ggcccccctt
     1381 ggatggcatt caaggagagg aaggtgtggg tgaaagagaa tatggtgttg aatctgtctt
     1441 gtgaagcgtc agggcacccc cggcccacca tctcctggaa cgtcaacggc acggcaagtg
     1501 aacaagacca agatccacag cgagtcctga gcaccctgaa tgtcctcgtg accccggagc
     1561 tgttggagac aggtgttgaa tgcacggcct ccaacgacct gggcaaaaac accagcatcc
     1621 tcttcctgga gctggtcaat ttaaccaccc tcacaccaga ctccaacaca accactggcc
     1681 tcagcacttc cactgccagt cctcatacca gagccaacag cacctccaca gagagaaagc
     1741 tgccggagcc ggagagccgg ggcgtggtca tcgtggctgt gattgtgtgc atcctggtcc
     1801 tggcggtgct gggcgctgtc ctctatttcc tctataagaa gggcaagctg ccgtgcaggc
     1861 gctcagggaa gcaggagatc acgctgcccc cgtctcgtaa gagcgaactt gtagttgaag
     1921 ttaagtcaga taagctccca gaagagatgg gcctcctgca gggcagcagc ggtgacaaga
     1981 gggctccggg agaccaggga gagaaataca tcgatctgag gcatggatcc ggtggaggta
     2041 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2101 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2161 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2221 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2281 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2341 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2401 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2461 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2521 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2581 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2641 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2701 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     2761 cggccgctcg acggtaccgc gggcccggga tcggccgcga
//
