LOCUS       sequence-418709-        8285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, PGK promoter, Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK GFP Blast (w510-5)
FEATURES             Location/Qualifiers
     source          1..8285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    135..260
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             307..487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(488..714)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(742..762)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(742..760)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(781..797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(781..797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(794..816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    805..821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(829..835,836..853,854..859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    874..895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1012..1029)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1183..1771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1263..1282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1942..2733,2734..2802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2565..2584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2803..2907)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2933..3388)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3070..3091)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3282..3301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3515..3537
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3529..3546
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3530..3546
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3556..3575
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3556..3574
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3595..3730
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3770..3891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3781..3800)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3835..3854
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3963..4196
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4283..4681)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(4700..4747)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4795..5124)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4809..4944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5109..5129
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    5180..5768
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5286..5297
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5695..5715
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5784..6497,6498..6500,6501..6503))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5787..6497,6498..6500,6501..6503))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5829..5850)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6178..6197
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6437..6458
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6500..6509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6525..7035)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6588..6606)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    7081..7198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7465..7509)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7694..7927
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
       61 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
      121 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
      181 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
      241 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
      301 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
      361 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
      421 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
      481 gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa tgcggcaatt
      541 cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag gcacgatcgt
      601 accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt
      661 gctaactcat cgttaccatg ttgcaagact acaagagtat tgcataagac tacattaagc
      721 ttgcagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
      781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      961 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1021 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1081 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1141 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1201 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1261 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1321 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1381 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1441 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1501 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1561 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1621 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1681 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1741 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1801 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1861 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1921 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1981 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2041 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2101 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2161 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2221 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2281 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2341 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2401 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2461 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2521 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2581 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2641 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2701 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2761 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     2941 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3001 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3061 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3121 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3181 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3241 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3301 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3361 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     3421 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     3481 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     3541 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacgtc cctaggcctc
     3601 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     3661 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     3721 ggggcgggat gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt
     3781 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3841 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact
     3901 ctctgatatt catttctttg caagttataa atactgaata ataagatgac atgaactact
     3961 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     4021 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     4081 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     4141 cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgagctagcc cttccagtcc
     4201 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     4261 aggtaccgag ctcgaattgt gcttagccct cccacacata accagagggc agcaattcac
     4321 gaatcccaac tgccgtcggc tgtccatcac tgtccttcac tatggctttg atcccaggat
     4381 gcagatcgag aagcacctgt cggcaccgtc cgcaggggct caagatgccc ctgttctcat
     4441 ttccgatcgc gacgatacaa gtcaggttgc cagctgccgc agcagcagca gtgcccagca
     4501 ccacgagttc tgcacaaggt cccccagtaa aatgatatac attgacacca gtgaagatgc
     4561 ggccgtcgct agagagagct gcgctggcga cgctgtagtc ttcagagatg gggatgctgt
     4621 tgattgtagc cgttgctctt tcaatgaggg tggattcttc ttgagacaaa ggcttggcca
     4681 tggtttagtt cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat
     4741 tgtcaacacg tgctgatcag atccgaaaat ggatatacaa gctcccggga gctttttgca
     4801 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggcagaggc
     4861 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     4921 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     4981 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     5041 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5101 ctttccacac cctaactgac acacattcca cagaattaat tccaaactca ttactaaccg
     5161 gatatctgca gaattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     5221 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
     5281 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
     5341 acacaggcga gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca
     5401 gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
     5461 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
     5521 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
     5581 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
     5641 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
     5701 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
     5761 ggttgattgt cgacgcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg
     5821 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     5881 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     5941 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
     6001 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
     6061 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     6121 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     6181 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     6241 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
     6301 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     6361 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     6421 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     6481 agctcctcgc ccttgctcac catggtggcg accggtggat ccccctgggg agagaggtcg
     6541 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg
     6601 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc
     6661 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc
     6721 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg
     6781 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga
     6841 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg
     6901 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc
     6961 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
     7021 cgcaacccca accccgtgga attcgatatc aagcttctcg aggctagtct cgtgatcgat
     7081 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
     7141 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaagt
     7201 taaccgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
     7261 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
     7321 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     7381 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     7441 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     7501 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     7561 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     7621 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     7681 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     7741 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     7801 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     7861 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     7921 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     7981 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     8041 gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct
     8101 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     8161 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     8221 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     8281 ctaac
//
