LOCUS       sequence-418714-        6417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty retroviral vector with IRES-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Kueh HY, Champhekar A, Nutt SL, Elowitz MB, Rothenberg EV
  TITLE     Positive feedback between PU.1 and the cell cycle controls myeloid 
            differentiation.
  JOURNAL   Science. 2013 Aug 9;341(6146):670-3. doi: 10.1126/science.1240831. 
            Epub 2013 Jul 18.
  PUBMED    23868921
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1240831"; journalName: "Science"; date: "2013-08-9-
            9"; volume: "341"; issue: "6146"; pages: "670-3"
COMMENT     Sequence Label: IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..6417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(405..1115)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(683..702)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     952..970
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1112..1121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1142..1694
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1144..1711
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1304..1323)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1533..1550
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(1763..2179)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1779..1795)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1811..1833)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2246..2587
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             2651..3167
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(3367..3386)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3514..3536)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3730..3749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3849..3871
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3909..3927)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3995..4099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4100..4168,4169..4960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4318..4337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5131..5719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5620..5639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5873..5890
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6007..6028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6043..6048,6049..6066,6067..6073)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6081..6097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             join(6259..6417,1..356)
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga gaagcgaact
       61 gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac cctggaaaca
      121 tctgatggtt ctctagaaac tgctgagggc gggaccgcat ctggggacca tctgttcttg
      181 gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca tattctgctg
      241 tctctctgtt cctaaccttg atctgaactt ctctattctc agttatgtat tttccatgcc
      301 ttgcaaaatg gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc
      361 ccctttttct ggagactaaa taaaatcttt tattttatcg atgcttactt gtacagctcg
      421 tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag
      481 tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc
      541 acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc
      601 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
      661 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
      721 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
      781 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
      841 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
      901 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc
      961 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     1021 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
     1081 atggccatgt tatcctcctc gcccttgctc accatggtgg cactagtcat ggttgtggcc
     1141 atattatcat cgtgttcttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg
     1201 tttttttaac ctcgactaaa cacatgtaaa gcatgtgtac cgaggcccca gatcagatcc
     1261 catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag
     1321 ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt
     1381 tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg
     1441 gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg
     1501 aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc
     1561 cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga
     1621 cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct
     1681 tcggccagta acgttagggg ggggggaggg agagggataa cttcgtatag cagaattcgt
     1741 taacctcgag agatctaatt ccggcgccta gagaaggagt gagggctgga taaagggagg
     1801 atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag
     1861 gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg
     1921 cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca ggtgaaaaga
     1981 ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc ggccatccga
     2041 cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc ttgacatcta
     2101 ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc aaacttaagg
     2161 gagtggtaac agtctggccc taattttcag acaaatacag aaacacagtc agacagagac
     2221 aacacagaac gatgctgcag cgctgcagca gacaagacgc gcggcttcgg ttccaaaccg
     2281 aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt ctcgtctcct accagaacca
     2341 catatcctga cggggtcgga ttccacatcg actcccttcc tcaggtcggg ccacaaaaac
     2401 ggcccccaaa gtccctggga cgtctcccag ggttgcggcc gggtgttcag aactcgtcag
     2461 ttccaccacg ggtccgccag atacagagct agttagctaa ctagtacaga cgcaggcgca
     2521 aacattagat gccggcacaa acacgcacaa agacagagac agacacgaaa cgaccgctgg
     2581 ccagcttacc tcccggcggg ggggtcggtg gtccctgggc aggggtctcc aaatctcggt
     2641 ggaacctcca aatgaaagac ccccgaggtg ggcagtcaat caatctgagg agaccctccc
     2701 aaggatcagc gagtccacga ttcggatgca aacagcaaga ggctttattg ggaatacggg
     2761 tacccgggcg acgcagtcta tcggaggact ggcgcgccga gtgaggggtt gtgggctctt
     2821 ttattgagct cggggagcag aagcgcgcga acagaagcga gaagcgaact gattggttag
     2881 ttcaaataag gcacagggtc atttcaggtc cttggggcac cctggaaaca tctgatggtt
     2941 ctctagaaac tgctgagggc gggaccgcat ctggggacca tctgttcttg gccctgagcc
     3001 ggggcaggaa ctgcttacca cagatatcct gtttggccca tattctgctg tctctctgtt
     3061 cctaaccttg atctgaactt ctctattctc agttatgtat tttccatgcc ttgcaaaatg
     3121 gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct
     3181 ggagactaaa taaaatcttt tattttatct atggctcgta ctctataggc ttcagctggt
     3241 gatattgttg agtcaaaact agagcctgga ccactgatat cctgtcttta acaaattgga
     3301 ctaatcgcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     3361 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     3421 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     3481 ccatctcctt gcatgcacca ttccttgcgc cgtcgtttta caacgtcgtg actgggaaaa
     3541 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3601 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     3721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3781 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3841 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3901 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3961 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4021 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4081 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4141 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4201 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4261 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4321 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4381 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4441 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4501 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4561 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4621 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4681 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4741 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4801 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4861 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4921 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4981 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5041 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5101 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5161 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5221 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5281 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5341 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5401 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5461 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5521 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5581 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5641 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5701 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5761 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5821 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5881 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5941 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6001 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6061 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacaattctc cccagctctt
     6121 tctgcgctgt ccataatgaa ctatttcagg agtattggag tatgtggatt caaaacagtg
     6181 gctaattgag tcaggattac ttaatattcg actcctaccc tcagaatgtt gttctccaac
     6241 ttcaagaaac tgttacccat gaaagacccc cgctgacggg tagtcaatca ctcagaggag
     6301 accctcccaa ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat
     6361 acacgggtac ccgggcgacg cagtctatcg gaggactggc gcgccgagtg aggggtt
//
