LOCUS       sequence-418730-        8659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting a control region at the 
            CXCR4 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W
FEATURES             Location/Qualifiers
     source          1..8659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(19..474)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(156..177)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     368..387
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     601..623
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     615..632
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     616..632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     642..661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        642..660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        976..1174
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1129..1149
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1192..1372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1419..1544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2043..2276
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2461..2505
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2803..2920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2995..3243
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2995..3015
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3166..3185
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3372..3871
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3407..3430)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3804..3823
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3911..4507
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3911..3930)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4407..4427
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4526..4579
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4580..5290
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4725..4743)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(5350..5370)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5768..5779)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6231..6248)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6237..6461
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6487..6507)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6487..6505)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6526..6542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6526..6542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6539..6561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6550..6566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6574..6580,6581..6598,6599..6604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6619..6640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6757..6774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6928..7516)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7008..7027)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7687..8478,8479..8547))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8310..8329
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8548..8652)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
       61 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      121 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
      181 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
      241 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
      301 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
      361 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
      421 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
      481 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      541 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      601 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
      661 gcgaattgac tagtacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      721 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      781 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      841 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      901 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      961 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     1021 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     1081 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     1141 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     1201 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1261 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1321 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1381 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1441 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1501 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1561 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1621 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1681 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1741 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1801 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1861 gtaagaccac cgcacagcaa gcggccggcc gcgctgatct tcagacctgg aggaggagat
     1921 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1981 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2041 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2101 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2161 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2221 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2281 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2341 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2401 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2461 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2521 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2581 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2641 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2701 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2761 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2821 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2881 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2941 cagagatcca gtttggttag taccgggccc tacgcgtcca aggtcgggca ggaagagggc
     3001 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     3061 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     3121 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     3181 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     3241 accgtttaaa gaccttacga ggcgtttaag agctatgctg gaaacagcat agcaagttta
     3301 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggat
     3361 ccaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     3421 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     3481 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     3541 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     3601 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     3661 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     3721 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     3781 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     3841 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct
     3901 gaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3961 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4021 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4081 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4141 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4201 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4261 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4321 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4381 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4441 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4501 cggtgccggc ggcgggtccg gaggagaggg cagaggaagt ctcctaacat gcggtgacgt
     4561 ggaggagaat cctggcccaa tggtgagcaa gggcgaggag gataactccg ccatcatcaa
     4621 ggagttcctg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     4681 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     4741 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     4801 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     4861 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     4921 ggactcctct ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     4981 cccctccgac ggccccgtaa tgcagaagaa aaccatgggc tgggaggcct cctccgagcg
     5041 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     5101 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     5161 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     5221 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     5281 gtacaagtga gcggccgctc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5341 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5401 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5461 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5521 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5581 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5641 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5701 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5761 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5821 gcctcttccg cgtctacgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5881 cccgcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5941 aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagtcaag atctgctttt
     6001 tgcctgtact gggtctctct ggttagacca gagtctctct ggttagacca gatctgagcc
     6061 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6121 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6181 cccttttagt cagtgtggaa aatctctagc agtttaaacc cgctgatcag cctcgactgt
     6241 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6301 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6361 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6421 agtcaatagc aggcatgctg gggatgcggt gggctctatg ggcggccgtt aattaagctt
     6481 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     6541 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga gcataaagtg
     6601 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6661 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6721 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6841 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6901 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6961 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7021 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7081 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7141 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7201 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7261 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7321 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7381 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7441 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7501 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7561 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7621 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7681 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7741 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7801 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7861 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7921 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7981 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8041 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8101 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8161 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8221 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8281 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8341 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8401 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8461 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8521 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8581 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8641 aggggttccg cgcacattt
//
