LOCUS       sequence-418735-        6998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, 
            DeCaprio JA, Weinberg RA
  TITLE     Enumeration of the simian virus 40 early region elements necessary 
            for human cell transformation.
  JOURNAL   Mol Cell Biol 2002 Apr;22(7):2111-23.
  PUBMED    11884599
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-04"; volume: "22(7)"; pages: "2111-23"
COMMENT     Sequence Label: pBABE-zeo largeTcDNA
FEATURES             Location/Qualifiers
     source          1..6998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    252..451
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             516..985
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        1016..1120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1121..1189,1190..1981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1339..1358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2141..2729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2630..2649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2883..2900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3030..3499
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(3670..4044)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4054..4383)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4068..4203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4368..4388
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(4469..6595)
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     CDS             complement(join(6633..6998,1..51))
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6649..6665)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6681..6703)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 atctttccag tgacctaagg tcaaacttaa gggagtggta acagtctggc cctaattttc
       61 agacaaatac agaaacacag tcagacagag acaacacaga acgatgctgc agcgctgcag
      121 cagacaagac gcgcggcttc ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa
      181 ctgttttagg ttctcgtctc ctaccagaac cacatatcct gacggggtcg gattccacat
      241 cgactccctt cctcaggtcg ggccacaaaa acggccccca aagtccctgg gacgtctccc
      301 agggttgcgg ccgggtgttc agaactcgtc agttccacca cgggtccgcc agatacagag
      361 ctagttagct aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc
      421 ggacagacac agataagttg ctggccagct tgcctcccgg tggtgggtcg gtggtccctg
      481 ggcaggggtc tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt
      541 caatcactca gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca
      601 agagggttta ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc
      661 cgagtgaggg gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag
      721 cgagaagcga actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg
      781 caccctggaa acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga
      841 ccatctgttc ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc
      901 ccatattcag ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca
      961 gatatcctgt ttggcccata ttcaggctgc aggtggcact tttcggggaa atgtgcgcgg
     1021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1141 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1201 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1261 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1321 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1381 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1441 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1501 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1561 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1621 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     1681 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1741 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     1801 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     1861 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1921 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1981 actgtcagac caagtttact catatatact ttagattgat ttgcggccgc aaaaggatct
     2041 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2281 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2461 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2521 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2581 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2641 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2701 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2761 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2821 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2881 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2941 cgcgttggcc gattcattaa tgcaactatg gccatttaat gtaaatactt aagaaaaaaa
     3001 accaaattaa ttttgataca tgctgcatgt gaaagacccc cgctgacggg tagtcaatca
     3061 ctcagaggag accctcccaa ggaacagcga gaccacaagt cggatgcaac tgcaagaggg
     3121 tttattggat acacgggtac ccgggcgact cagtcaatcg gaggactggc gccccgagtg
     3181 aggggttgtg ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa
     3241 gcgaactgat tggttagttc aaataaggca cagggtcatt tcaggtcctt ggggcaccct
     3301 ggaaacatct gatggttctc tagaaactgc tgagggctgg accgcatctg gggaccatct
     3361 gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat
     3421 tcagctgttc catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc
     3481 ctgtttggcc catattcagc tgttccatct gttcctgacc ttgatctgaa cttctctatt
     3541 ctcagttatg tatttttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac
     3601 ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat cttttatttt
     3661 atcgatcggt cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg
     3721 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
     3781 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
     3841 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
     3901 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     3961 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     4021 aacggcactg gtcaacttgg ccatggtaag ctttttgcaa aagcctaggc ctccaaaaaa
     4081 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     4141 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     4201 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     4261 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     4321 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     4381 cacattccac agggtcgacc actgtgctgg cgaattccta cgtaccacca cactgggatc
     4441 cccgttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     4501 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcatgaacag
     4561 actgtgagga ctgaggggcc tgaaatgagc cttgggactg tgaatcaatg cctgtttcat
     4621 gccctgagtc ttccatgttc ttctccccac catcttcatt tttatcagca ttttcctggc
     4681 tgtcttcatc atcatcatca ctgtttctta gccaatctaa aactccaatt cccatagcca
     4741 cattaaactt cattttttga tacactgaca aactaaactc tttgtccaat ctctctttcc
     4801 actccacaat tctgctctga atactttgag caaactcagc cacaggtctg taccaaatta
     4861 acataagaag caaagcaatg ccactttgaa ttattctctt ttctaacaaa aactcactgc
     4921 gttccaggca atgctttaaa taatctttgg gcctaaaatc tatttgtttt acaaatctgg
     4981 cctgcagtgt tttaggcaca ctgtactcat tcatggtgac tattccaggg ggaaatattt
     5041 gagttctttt atttaggtgt ttcttttcta agtttacctt aacactgcca tccaaataat
     5101 cccttaaatt gtccaggtta ttaattccct gacctgaagg caaatctctg gactcccctc
     5161 cagtgccctt tacatcctca aaaactacta aaaactggtc aatagctact cctagctcaa
     5221 agttcagcct gtccaagggc aaattaacat ttaaagcttt ccccccacat aattcaagca
     5281 aagcagctgc taatgtagtt ttaccactat caattggtcc tttaaacagc cagtatcttt
     5341 ttttaggaat gttgtacacc atgcatttta aaaagtcata caccactgaa tccattttgg
     5401 gcaacaaaca gtgtagccaa gcaactccag ccatccattc ttctatgtca gcagagcctg
     5461 tagaaccaaa cattatatcc atcctatcca aaagatcatt aaatctgttt gttaacattt
     5521 gttctctagt taattgtagg ctatcaaccc gctttttagc taaaacagta tcaacagcct
     5581 gttggcatat ggttttttgg tttttgctgt cagcaaatat agcagcattt gcataatgct
     5641 tttcatggta cttatagtgg ctgggctgtt cttttttaat acattttaaa cacatttcaa
     5701 aactgtactg aaattccaag tacatcccaa gcaataacaa cacatcatca cattttgttt
     5761 ccattgcata ctctgttaca agcttccagg acacttgttt agtttcctct gcttcttctg
     5821 gattaaaatc atgctccttt aacccacctg gcaaactttc ctcaataaca gaaaatggat
     5881 ctctagtcaa ggcactatac atcaaatatt ccttattaac ccctttacaa attaaaaagc
     5941 taaaggtaca caatttttga gcatagttat taatagcaga cactctatgc ctgtgtggag
     6001 taagaaaaaa cagtatgtta tgattataac tgttatgcct acttataaag gttacagaat
     6061 atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa
     6121 agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag
     6181 gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt
     6241 cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat
     6301 taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc tcagttgcat
     6361 cccagaagcc tccaaagtca ggttgatgag catattttac tccatcttcc attttcttgt
     6421 acagagtatt cattttcttc attttttctt catctcctcc tttatcagga tgaaactcct
     6481 tgcatttttt taaatatgcc tttctcatca gaggaatatt cccccaggca ctcctttcaa
     6541 gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta tccatctttg
     6601 caaagctggt cgaccggtcg cgggatccgg ccggcgccta gagaaggagt gagggctgga
     6661 taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat
     6721 ggaggaagag gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca
     6781 aaagccaagg cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca
     6841 ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc
     6901 ggccatccga cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc
     6961 ttgacatcta ccgactggtt gtgagcgatc cgctcgac
//
