LOCUS       sequence-418760-        7834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (RFP)
FEATURES             Location/Qualifiers
     source          1..7834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    630..755
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             802..982
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(983..1209)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1237..1257)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1237..1255)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1276..1292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1276..1292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1289..1311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1300..1316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1324..1330,1331..1348,1349..1354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1369..1390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1507..1524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1678..2266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1758..1777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2437..3228,3229..3297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3060..3079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3298..3369)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3369..3504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3544..3665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3555..3574)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3609..3628
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3737..3970
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             4175..4186
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4584..4604
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4644..4652,4653..4688,4689..4691,4692..4727,
                     4728..4730,4731..4766,4767..4772,4773..5390))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(5391..5444)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5460..6152)
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     promoter        complement(6176..6686)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6239..6257)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(6714..6770)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(6811..7125)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(6814..6838)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6885..6903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6921..6939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6956..6974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6992..7010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7028..7046
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7063..7081
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7099..7117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    7179..7296
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7509..7553)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(7738..7834,1..137)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
       61 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
      121 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
      181 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
      241 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt
      301 gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
      361 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
      421 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
      481 tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat
      541 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
      601 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
      661 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
      721 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
      781 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
      841 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
      901 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
      961 tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc
     1021 tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta
     1081 ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc
     1141 cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat
     1201 aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg
     1261 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1321 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1381 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1441 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1501 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1561 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1621 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2101 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2221 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2281 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2341 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2401 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2461 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2521 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2581 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2641 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2701 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2761 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2821 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2881 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2941 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3001 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3061 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3121 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3181 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3241 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3301 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3361 atttgaaagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     3421 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     3481 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggatagcta gagccagaca
     3541 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3601 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3661 aagttcctct cactctctga tattcatttc tttgcaagtt ataaatactg aataataaga
     3721 tgacatgaac tactactgct agagattttc cacactgact aaaagggtct gagggatctc
     3781 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     3841 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     3901 tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg ggagtgaatt
     3961 agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa
     4021 gtcattggtc ttaaaggtac ctctagtccg gacgccgagg cgaaacaggc ggggaggcgg
     4081 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag
     4141 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga
     4201 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca
     4261 gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc
     4321 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc
     4381 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca
     4441 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca
     4501 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca
     4561 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaatacgat
     4621 atcttggatc gatccttact tagttacccg gggagcatgt caaggtcaaa atcgtcaaga
     4681 gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct
     4741 aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc
     4801 aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg
     4861 aacagctcaa ttgcttgtct cagaagtggg ggcatagaat cggtggtagg tgtctctctt
     4921 tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc
     4981 acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg
     5041 agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta
     5101 gccccgtcgc gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag
     5161 tcttgccatg actcgccttc cagggggcag aagtgggtat gatgcctgtc cagcatctcg
     5221 attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc
     5281 tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gacaccattg
     5341 agtaattcca gagctccgtt tatgactttg ctcttgtcca gtctagacat tggaccaggg
     5401 ttttcttcaa catcaccaca agtgaggaga gaacctctac cttctccgct tccttcgaat
     5461 ctgtgcccca gtttgctagg gaggtcgcag tacttggcca cagccatctc gtgctgctcg
     5521 acgtaggtct ctttgtcggc ctccttgatt ctttccagtc tgtggtccac gaagtggaag
     5581 ccgggcatct tgaggttctt agcgggtttc ttggatctgt atgtggtctt gaaggagcag
     5641 tgcaggtagc ccccgcccac gagcttcagg gccatctggc tgtggcctct caggccgccg
     5701 tcagcggggt acagcatctc ggtgttggcc tcccagccgc gtgttttctt ctgcatcaca
     5761 gggccgttgg atgggaagtt caccccgttg atcttgacgt tgtagatgat gcagccgttc
     5821 tggaagctgg tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgattctc
     5881 tcccatgtga agccctcagg gaaggactgc ttaaagaagt cggggatgcc ctgggtgtgg
     5941 ttgatgaagg ctttgctgcc gtacatgaag ctggtagcca ggatgtcgaa ggcgaagggg
     6001 agagggccgc cctcgaccac cttgatcttc atggtctggg tgccctcgta gggcttgcct
     6061 tcgccctcgg atgtgcactt gaagtggtgg ttgttcacgg tgccctccat gtacagcttc
     6121 atgtgcatgt tctccttgat cagctcgctc atggtggccg cgcgcgggtg aattgctggg
     6181 gagagaggtc ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct
     6241 tgcagaatgc ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg
     6301 cctcccgcac cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat
     6361 tggccacagc ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga
     6421 gggtactagt gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg
     6481 aaccttcccg acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg
     6541 cgaagatccg ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc
     6601 cgctgcgttt cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc
     6661 cttggaaaag gcgcaacccc aaccccggat cctgtacaac gcgtctgcag cctaggaccg
     6721 gggttttctt ccacgtctcc tgcttgcttt aacagagaga agttcgtggc tccggaaccg
     6781 gtgtcgacga attcgctagc caattctcca ggcgatctga cggttcacta aacgagctct
     6841 gcttatatag gcctcccacc gtacacgcct acctcgacat acgttctcta tcactgatag
     6901 ggagtaaact cgacatacgt tctctatcac tgatagggat aaactcgaca tacgttctct
     6961 atcactgata gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga
     7021 catacgttct ctatcactga tagggagtaa actcgacatc gttctctatc actgataggg
     7081 agtaaactcg acatacgttc tctatcactg atagggagta aactcgacat atcgattcgc
     7141 ggccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     7201 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     7261 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     7321 ctcgaggtcg agaattgtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
     7381 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
     7441 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
     7501 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
     7561 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
     7621 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
     7681 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
     7741 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
     7801 ccccagactg tgagttgcaa cagatgctgt tgcg
//
