LOCUS       sequence-418763-        6222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bidirectional CAG promoter (compatible with the MXS Chaining Kit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     Sequence Label: MXS_BidirectionalCAG
FEATURES             Location/Qualifiers
     source          1..6222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          101..1117
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     enhancer        1396..1775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        1783..2162
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2170..2549
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2557..2936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3224..4240
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        4330..4434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4435..4503,4504..5295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4653..4672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5466..6054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5955..5974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgaagc ttacgcgtaa cctcgagcca tggaacgaat tctttgccaa
       61 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
      121 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
      181 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
      241 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
      301 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt
      361 tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata
      421 aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgcyccgg gggccgccgc
      481 gccccycccc cgagcccycc ccggcccgrg gcggccccgc cccgcccggc acccccacct
      541 gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac
      601 ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga
      661 ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt
      721 tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca
      781 cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg
      841 cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc
      901 ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa
      961 caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc
     1021 tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg
     1081 agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt
     1141 tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct
     1201 gattggctgc cgccscmccy cyccsccycs ccccsccccc cccccccccc cccccccccc
     1261 ccccggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
     1321 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
     1381 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     1441 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     1501 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     1561 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     1621 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     1681 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     1741 tgattactat taataactag tcaataatca atgtcgagaa cccatggtaa tagcgatgac
     1801 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     1861 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     1921 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     1981 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2041 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     2101 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     2161 tcgagaaccc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     2221 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     2281 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     2341 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     2401 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     2461 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     2521 ctattaataa ctagtcaata atcaatgtcg agaacccatg gtaatagcga tgactaatac
     2581 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     2641 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     2701 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     2761 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     2821 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     2881 atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcgagc
     2941 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     3001 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     3061 gggggggcgc gcgccrggsg gggsggggsg gggsgggggg gggggngggg sgrggsggrg
     3121 rggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     3181 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     3241 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     3301 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     3361 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     3421 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     3481 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     3541 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     3601 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     3661 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     3721 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     3781 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc cycgggccgg
     3841 ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     3901 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     3961 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     4021 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     4081 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     4141 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     4201 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     4261 tattgtgctg tctcatcatt ttggcaaaga attcgtcgac aagcttctcg accaattcgg
     4321 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4381 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4441 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4501 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4561 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4621 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4681 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4741 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4801 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4861 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4921 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4981 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5041 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5101 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5161 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5221 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5281 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5341 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5401 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5461 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5521 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5581 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5641 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5701 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5761 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5821 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5881 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5941 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6001 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6061 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6121 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6181 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gc
//
