LOCUS       sequence-418769-        8346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAAV-RAM-NLS-mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
FEATURES             Location/Qualifiers
     source          1..8346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      138..592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(225..244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     434..455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        618..722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(723..791,792..1583)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(941..960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1754..2342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2243..2262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2647..2871
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2938..3471)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3641..3646,3647..3663,3664..3669))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   4009..4138
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        4156..4470
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4164..4182
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4200..4218
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4235..4253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4271..4289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4307..4325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4342..4360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4378..4396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4443..4467
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4488..4508
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4509..5204
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     misc_feature    5257..5845
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5310..5330)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5728..5739)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5878..5895)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5884..5995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    6143..6191
                     /note="synthetic polyadenylation signal"
     misc_feature    6205..6296
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6245..6264
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             join(6653..7270,7271..7276,7277..7312,7313..7315,7316..7351,
                     7352..7354,7355..7390,7391..7399)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7416..7432)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7457..7476)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7458..7476)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7502..7636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7665..7805
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7665..7794
                     /label=AAV2 ITR
     polyA_signal    7972..8106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8135..8300
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 aactccatgt gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt
       61 ttctgtcatc tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag
      121 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      181 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      421 cacgttctta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      481 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
      541 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      841 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      901 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      961 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1021 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1081 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1141 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1201 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1261 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1321 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1381 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1441 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1501 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1561 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1621 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1681 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1861 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2161 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2221 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2281 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2341 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2401 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2461 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2521 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag
     2581 accagccgcg taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta
     2641 agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa
     2701 taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa
     2761 aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg
     2821 cccgtcgtat taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg
     2881 tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta
     2941 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     3001 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     3061 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     3121 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt
     3181 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     3241 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     3301 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     3361 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     3421 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     3481 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
     3541 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     3601 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
     3661 ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
     3721 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt
     3781 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac
     3841 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt
     3901 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt
     3961 cgcccaggac tctagctata gttctagtgg ttggctacag cttgcatgcc tgcaggcagc
     4021 tgcgcgctcg ctcgctcact gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc
     4081 tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctgc
     4141 ggccgcacgc gtctcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     4201 ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg
     4261 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     4321 agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc
     4381 ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc
     4441 agagctcgtt tagtgaaccg tcagatcgcc accggtgttt aatggccccc aagaagaaga
     4501 ggaaggtggt gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca
     4561 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     4621 cccagaccat gagaatcaag gcggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     4681 tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa ccacacccag ggcatccccg
     4741 acttctttaa gcagtccttc cccgagggct tcacatggga gagagtcacc acatacgaag
     4801 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     4861 acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     4921 tcggctggga ggcctccacc gagaccctgt accccgctga cggcggcctg gaaggcagag
     4981 ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttg aagaccacat
     5041 acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat gtggacagaa
     5101 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg
     5161 tggccagata ctgcgacctc cctagcaaac tggggcacag atgagtcgac ggcgcgccag
     5221 cgatccaccg gatctagata actgatcctt atcgataatc aacctctgga ttacaaaatt
     5281 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5341 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5401 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5461 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5521 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5581 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5641 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     5701 cgcgggacgc ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5761 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5821 atctcccttt gggccgcctc cccgcatcga taccgtcgac tagagctcgc tgatcagcct
     5881 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5941 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6001 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6061 attgggaaga caatagcaga ctagtacgtc tctcaaggat aagtaagtaa tattaaggta
     6121 cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     6181 ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa
     6241 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctggcc
     6301 taactggccg gtaccctaga agtttgttcg tgactcagac tagaagtttg ttcgtgactc
     6361 agactagaag tttgttcgtg actcagacta gaagtttgtt cgtgactcag aactcattca
     6421 taaaacgctt gttataaaag cagtggctgc ggcgcctcgt actccaaccg catctgcagc
     6481 gagcaactga gaagccaaga ctgagccggc ggccgagctc atgagccatg gcttcccgcc
     6541 ggaggtggag gagcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat
     6601 ggaccgtcac cctgcagcct gtgcttctgc taggatcaat gtggaattca ccatgtctag
     6661 actggacaag agcaaagtca taaactctgc tctggaatta ctcaatgaag tcggtatcga
     6721 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     6781 gcacgtgaag aacaagcggg ccctgctcga tgccctggca atcgagatgc tggacaggca
     6841 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     6901 caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     6961 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     7021 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     7081 ctgcgtattg gaggatcagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     7141 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccatc agggagccga
     7201 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     7261 cgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga
     7321 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     7381 tgacatgctc cccgggtaac taagtaagga tccatcgata ccgtcgacct cgaggggggg
     7441 cccggtaccc aattcgccct atagtgagtc gtattacgcg cgcagcggcc gaccatggcc
     7501 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7621 ttatcatgtc tggatctccg gacacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     7681 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     7741 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     7801 gcaggtctga gacaataacc ctgataaatg cttcaataat gtaagcttgt cgagaagtac
     7861 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     7921 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     7981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8101 tggatctgat cactgatatc gcctaggaga tccgaaccag ataagtgaaa tctagttcca
     8161 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     8221 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     8281 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat
     8341 cctgcc
//
