LOCUS       sequence-418815-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 Delta RBD (receptor-binding domain from Spike) 
            with N-terminal SpyTag fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP,
            Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo 
            J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, 
            Howarth MR
  TITLE     Proactive vaccination using multiviral Quartet Nanocages to elicit 
            broad anti-coronavirus responses.
  JOURNAL   Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9.
  PUBMED    38710880
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9."
COMMENT     Sequence Label: SpyTag-RBD Delta
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..111
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gcgcccacat cgtgatggtg gacgcctaca agcctaccaa aggcagcggt
      121 ggaagcggcg gatctggaac cggtaacata acaaacctct gtccctttgg ggaagtattc
      181 aatgcaactc gcttcgcatc cgtttatgcg tggaacagaa aaagaatctc caactgtgtc
      241 gcagactact ccgtacttta caacagcgca tcatttagca ctttcaagtg ttacggcgtt
      301 agtcccacta agctgaatga cttgtgcttt actaacgttt atgctgactc cttcgtaatt
      361 cgcggcgacg aagtcagaca gattgctcca ggacagacag gaaagattgc agactacaac
      421 tataagttgc ctgacgactt tactggatgc gtgatagctt ggaattcaaa taatcttgac
      481 tcaaaagtcg gtggaaacta taactaccga tatagactgt ttcgcaagtc taaccttaaa
      541 ccctttgaga gggatatttc aactgagata tatcaagctg gttctaaacc ctgtaatggg
      601 gttgaaggat tcaactgcta ttttccgttg cagtcatatg gttttcaacc aacgaacggt
      661 gttggctatc agccctatcg ggtggtcgta ctctcattcg agctgttgca cgcgccggct
      721 acggtgtgtg gtccaaagaa gtag
//
