LOCUS       sequence-418817-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain 
            from Spike) with N-terminal SpyTag fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP,
            Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo 
            J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, 
            Howarth MR
  TITLE     Proactive vaccination using multiviral Quartet Nanocages to elicit 
            broad anti-coronavirus responses.
  JOURNAL   Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9.
  PUBMED    38710880
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9."
COMMENT     Sequence Label: SpyTag-RBD Omicron XBB.1.5
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..111
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gcgcccacat cgtgatggtg gacgcctaca agcctaccaa aggcagcggt
      121 ggaagcggcg gatctggaac cggtaatata acgaatctgt gccccttcca cgaggtcttt
      181 aacgccacca cattcgcgag tgtttacgcg tggaacagaa agcggatatc caactgtgtt
      241 gccgattata gcgtcatcta taattttgcc ccgttctttg cctttaagtg ttatggggtg
      301 agtccgacga aattgaacga tctttgcttc acgaacgttt atgcggactc cttcgtaata
      361 cgaggcaatg aggtaagtca gatagctccc ggacagactg ggaatattgc ggactacaat
      421 tacaaacttc cagacgattt taccggatgc gtaattgcct ggaacagtaa taaactggat
      481 agtaaaccta gtggtaatta caattatctc tacagattgt ttaggaaaag caagcttaaa
      541 ccgttcgaaa gagacatatc cacggaaata tatcaggccg ggaacaaacc atgcaatggg
      601 gttgccggcc caaactgtta tagcccactg caatcatacg gctttcgccc tacatatggc
      661 gtcgggcacc agccgtacag agtggtagtt ctcagcttcg agctccttca cgcaccggct
      721 acggtgtgcg gaccgaaaaa gtag
//
