LOCUS       sequence-418820-        2763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In mammalian cells expresses a Quartet of receptor-binding domains 
            (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and 
            SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP,
            Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo 
            J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, 
            Howarth MR
  TITLE     Proactive vaccination using multiviral Quartet Nanocages to elicit 
            broad anti-coronavirus responses.
  JOURNAL   Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9.
  PUBMED    38710880
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9."
COMMENT     Sequence Label: SpyTag-Quartet
FEATURES             Location/Qualifiers
     source          1..2763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..111
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             complement(2483..2500)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gcgcccacat cgtgatggtg gacgcctaca agcctaccaa aggcagcggt
      121 ggaagcggcg gatctggaac cggtagagtg gcccctagca aagaagtcgt gcggttcccc
      181 aatatcacca atctgtgccc cttcggcgag gtgttcaacg ccaccacatt tccctctgtg
      241 tacgcctggg agagaaagcg gatcagcaac tgcgtggccg actacagcgt gctgtacaac
      301 agcacctcct ttagcacctt caagtgctac ggcgtgtccg ccaccaagct gaacgatctg
      361 tgcttctcca acgtgtacgc cgacagcttc gtggtcaagg gcgacgatgt gcggcagatt
      421 gctcctggac agacaggcgt gatcgccgat tacaactaca agctgcctga cgacttcctg
      481 ggctgtgtgc tggcctggaa caccaatagc aaggacagct ccaccagcgg caactacaac
      541 tacctgtaca gatgggtccg acgcagcaag ctgaatccct acgagaggga cctgagcaac
      601 gacatctata gccctggcgg ccagagctgt tctgccgtgg gacccaattg ctacaaccct
      661 ctgaggccct acggcttctt taccacagct ggcgtgggcc accagcctta cagagtggtg
      721 gtgctgtcat tcgagctgct gaatgcccct gccacagtgt gtggccctaa gctgtctacc
      781 gacctgatca agaaccaggg gagtggtggg tccgggggtt cgggacgggt gtcccctaca
      841 cacgaggtcg tgcggttccc caatatcacc aacagatgcc ccttcgacaa ggtgttcaac
      901 gccagcagat tccccaacgt gtacgcctgg gagcgcacca agatcagcga ttgcgtggcc
      961 gattacaccg tgctgtacaa ctccaccagc tttagcacct ttaagtgcta cggcgtgtcc
     1021 ccaagcaagc tgatcgacct gtgcttcacc tctgtgtacg ccgacacctt cctgatccgg
     1081 tctagcgaag tgcgacaagt ggcccctggc gaaacaggcg tgatcgccga ctacaactac
     1141 aagctgcccg acgacttcac cggctgtgtg atcgcctgga ataccgccaa gcaggaccag
     1201 ggccagtact actacagaag cagcagaaag accaagctca agcccttcga gcgggacctg
     1261 acctccgatg agaatggcgt cagaaccctg tccacctacg atttctaccc caatgtgccc
     1321 atcgagtacc aggccaccag agtggtggtg ctgtctttcg aactgctgaa cgcccctgcc
     1381 acagtgtgtg gccctaaact gtctacagcc ctggtcaaga accagtgcgt gaacttcggc
     1441 agcggcggaa cgggaggtag tggccgggtg cagcccaccg atagcatcgt gcggttcccc
     1501 aatatcacca atctgtgccc cttcggcgag gtgttcaatg ccaccacatt cgcctctgtg
     1561 tacgcctgga accggaagcg gatcagcaat tgcgtggccg actactccgt gctgtacaac
     1621 agcaccagct tcagcacctt caagtgctac ggcgtgtccc ctaccaagct gaacgacctg
     1681 tgcttcacaa acgtgtacgc cgacagcttc gtgatcaccg gggatgaagt gcggcagatt
     1741 gcccctggac agacaggcaa gatcgccgac tacaactaca agctgcccga cgacttcacc
     1801 ggctgtgtga ttgcctggaa ctccaagcac atcgacgcca aagaaggcgg gaacttcaac
     1861 tacctgtacc ggctgttccg gaaggccaac ctgaagcctt tcgagcggga tatctccacc
     1921 gagatctacc aggccggcag caagccttgt aatggccaga caggcctgaa ctgctactac
     1981 ccactgtaca gatacggctt ctaccccaca gacggcgtgg gccaccagcc ttatagagtg
     2041 gtggtgctga gcttcgagct gctgaatgcc cctgccacag tgtgcggccc taagaaaagc
     2101 accaatctcg tgaagaacaa atgcgtgaac ttcggtacgg gtggcagtgg agggagcggg
     2161 aacatcacca atctgtgccc cttcggcgag gtgttcaatg ccacaagatt tgccagcgtg
     2221 tacgcctgga accggaagag aatcagcaac tgcgtggccg actacagcgt gctgtacaat
     2281 agcgccagct tcagcacctt caagtgctac ggcgtgtccc ctaccaagct gaacgacctg
     2341 tgcttcacca atgtgtacgc cgacagcttc gtgatcagag gcgacgaagt tcggcagatc
     2401 gctcctggac agacaggcaa gatcgccgat tacaactaca agctgcccga cgacttcacc
     2461 ggctgcgtga tcgcctggaa tagcaacaac ctggacagca aagtcggcgg caactacaac
     2521 tacctgtacc ggctgttccg gaagtccaac ctgaagcctt tcgagcggga catcagcacc
     2581 gagatctatc aggccggcag caccccttgt aatggcgtgg aaggcttcaa ctgctacttc
     2641 ccactgcagt cctacggctt ccagcctaca aatggcgtcg gctaccagcc ttacagagtg
     2701 gtggtgctgt ccttcgaact gctgcatgcc cctgctaccg tgtgcggccc taaaaaaacc
     2761 tag
//
