LOCUS       sequence-418821-        2787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In mammalian cells expresses a Quartet of receptor-binding domains 
            (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each 
            separated by GS-linkers with an N-terminal SpyTag fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP,
            Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo 
            J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, 
            Howarth MR
  TITLE     Proactive vaccination using multiviral Quartet Nanocages to elicit 
            broad anti-coronavirus responses.
  JOURNAL   Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9.
  PUBMED    38710880
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9."
COMMENT     Sequence Label: SpyTag-Alternate Quartet
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..111
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gcgcccacat cgtgatggtg gacgcctaca agcctaccaa aggcagcggt
      121 ggaagcggcg gatctggaac cggtcgggtg cagcccacca tcagcatcgt gcggttcccc
      181 aatatcacca atctgtgccc cttcggcgag gtgttcaacg ccagcaagtt tgcctctgtg
      241 tacgcctgga accggaagcg gatcagcaat tgcgtggccg actacagcgt gctgtacaac
      301 agcaccagct tcagcacctt caagtgctac ggcgtgtccc ctaccaagct gaacgacctg
      361 tgcttcacaa acgtgtacgc cgacagcttc gtggtcaagg gcgacgaagt tcggcagatt
      421 gcccctggac agactggcgt gatcgccgat tacaactaca agctgcccga cgacttcacc
      481 ggctgtgtga ttgcctggaa cagcgtgaag caggatgccc tgacaggcgg caattacggc
      541 tacctgtacc ggctgttcag aaagagcaag ctgaagccct tcgagcggga catctccacc
      601 gagatctatc aggccggcag caccccttgc aatggccaag ttggcctgaa ctgctactac
      661 cctctggaaa gatacggctt tcaccccacc accggcgtga actaccagcc attcagagtg
      721 gtggtgctga gcttcgagct gctgaatgga cctgccacag tgtgcggccc taagctgtct
      781 acaaccctgg tcaaggataa gtgcgtgaac ttcggctcag gtggatccgg aggtacggga
      841 agaatcctgc ctagcacaga ggtcgtgcgg ttccccaaca tcaccaattt ctgccccttc
      901 gacaaggtgt tcaacgccac cagatttccc aacgtgtacg cctggcagcg gaccaagatc
      961 agcgactgta tcgccgacta caccgtgctg tacaacagca ccagcttcag caccttcaag
     1021 tgctacggcg tgtcccctag caagctgatc gacctgtgct tcacctctgt gtacgccgac
     1081 accttcctga tccggttctc tgaagtgcgg cagatcgccc ctggcgaaac aggcgtgatc
     1141 gccgattaca actacaagct gcccgacgac ttcaccggct gtgtgctggc ttggaatact
     1201 gcccagcagg acatcggcag ctacttctac agatcccacc gggccgtgaa gctgaagccc
     1261 ttcgagagag atctgagcag cgacgagaat ggcgtgcgga ccctgagcac ctacgacttc
     1321 aaccccaatg tgcccctgga ctaccaggcc acaagagtgg tggtgctgag cttcgagctg
     1381 ctgaatgccc ctgccacagt gtgtggccct aagctgtcta cccagctggt caagaacaga
     1441 tgcgtgaact tcgggtccgg tgggtcgggc ggtagtggga gagtgtctcc tgtgacagag
     1501 gtcgtgcggt tccccaacat caccaatctg tgccccttcg acaaggtgtt caacgccacc
     1561 agatttccca gcgtgtacgc ctgggagcgc accaagatct ctgattgcgt ggccgactac
     1621 accgtgttct acaacagcac cagcttcagc accttcaact gctacggcgt gtcccctagc
     1681 aagctgatcg acctgtgctt cacctctgtg tacgccgaca ccttcctgat ccggttcagc
     1741 gaagtgcgac aggtggcacc tggacagaca ggcgtgatcg ccgattacaa ctacaagctg
     1801 cccgacgact tcaccggctg tgtgatcgcc tggaataccg ccaagcagga cgtgggcagc
     1861 tacttctaca gaagccacag aagctccaag ctgaagccct tcgagaggga cctgagcagc
     1921 gaagaaaatg gcgtgcggac cctgagcacc tacgacttca accagaacgt gcccctggaa
     1981 tatcaggcca ccagagtggt ggtgctgagc ttcgagctgc tgaatgcccc tgccacagtg
     2041 tgtggcccta agctgtctac cagcctggtc aagaaccagt gcgtgaactt cggtagcggg
     2101 gggtcggggg gcaccggcag agtggcccct agcaaagaag tcgtgcggtt ccccaatatc
     2161 accaatctgt gccccttcgg cgaggtgttc aacgccacca catttccctc tgtgtacgcc
     2221 tgggagagaa agcggatcag caactgcgtg gccgactaca gcgtgctgta caacagcacc
     2281 tcctttagca ccttcaagtg ctacggcgtg tccgccacca agctgaacga tctgtgcttc
     2341 tccaacgtgt acgccgacag cttcgtggtc aagggcgacg atgtgcggca gattgctcct
     2401 ggacagacag gcgtgatcgc cgattacaac tacaagctgc ctgacgactt caccggctgt
     2461 gtgctggcct ggaacaccag aaacatcgac gccacacaga ccggcaacta caattacaag
     2521 tacagaagcc tgcggcacgg caagctgagg cctttcgaga gggacatcag caatgtgcct
     2581 ttcagccccg acggcaagcc ttgtacacct cctgctttca actgctactg gcccctgaac
     2641 gactacggct tctacatcac aaacggcatc ggctatcagc cctacagagt ggtggtgctg
     2701 tccttcgagc tgctgaatgc ccctgccaca gtgtgtggcc ctaagctgtc taccgacctg
     2761 atcaagaacc agtgcgtgaa cttctag
//
