LOCUS       sequence-418824-        2682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In mammalian cells expresses a Quartet of receptor-binding domains 
            (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and 
            SARS-CoV-2 with no linkers between the RBDs and an N-terminal 
            SpyTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Hills RA, Tan TK, Cohen AA, Keeffe JR, Keeble AH, Gnanapragasam PNP,
            Storm KN, Rorick AV, West AP Jr, Hill ML, Liu S, Gilbert-Jaramillo 
            J, Afzal M, Napier A, Admans G, James WS, Bjorkman PJ, Townsend AR, 
            Howarth MR
  TITLE     Proactive vaccination using multiviral Quartet Nanocages to elicit 
            broad anti-coronavirus responses.
  JOURNAL   Nat Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9.
  PUBMED    38710880
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Nanotechnol. 2024 May 6. doi: 10.1038/s41565-024-01655-9."
COMMENT     Sequence Label: SpyTag-Quartet_NoLinker
FEATURES             Location/Qualifiers
     source          1..2682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..111
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             complement(2402..2419)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gcgcccacat cgtgatggtg gacgcctaca agcctaccaa aggcagcggt
      121 ggaagcggcg gatctggaac cggtagagtg gcccctagca aagaagtcgt gcggttcccc
      181 aatatcacca atctgtgccc cttcggcgag gtgttcaacg ccaccacatt tccctctgtg
      241 tacgcctggg agagaaagcg gatcagcaac tgcgtggccg actacagcgt gctgtacaac
      301 agcacctcct ttagcacctt caagtgctac ggcgtgtccg ccaccaagct gaacgatctg
      361 tgcttctcca acgtgtacgc cgacagcttc gtggtcaagg gcgacgatgt gcggcagatt
      421 gctcctggac agacaggcgt gatcgccgat tacaactaca agctgcctga cgacttcctg
      481 ggctgtgtgc tggcctggaa caccaatagc aaggacagct ccaccagcgg caactacaac
      541 tacctgtaca gatgggtccg acgcagcaag ctgaatccct acgagaggga cctgagcaac
      601 gacatctata gccctggcgg ccagagctgt tctgccgtgg gacccaattg ctacaaccct
      661 ctgaggccct acggcttctt taccacagct ggcgtgggcc accagcctta cagagtggtg
      721 gtgctgtcat tcgagctgct gaatgcccct gccacagtgt gtggccctaa gctgtctacc
      781 gacctgatca agaaccagcg ggtgtcccct acacacgagg tcgtgcggtt ccccaatatc
      841 accaacagat gccccttcga caaggtgttc aacgccagca gattccccaa cgtgtacgcc
      901 tgggagcgca ccaagatcag cgattgcgtg gccgattaca ccgtgctgta caactccacc
      961 agctttagca cctttaagtg ctacggcgtg tccccaagca agctgatcga cctgtgcttc
     1021 acctctgtgt acgccgacac cttcctgatc cggtctagcg aagtgcgaca agtggcccct
     1081 ggcgaaacag gcgtgatcgc cgactacaac tacaagctgc ccgacgactt caccggctgt
     1141 gtgatcgcct ggaataccgc caagcaggac cagggccagt actactacag aagcagcaga
     1201 aagaccaagc tcaagccctt cgagcgggac ctgacctccg atgagaatgg cgtcagaacc
     1261 ctgtccacct acgatttcta ccccaatgtg cccatcgagt accaggccac cagagtggtg
     1321 gtgctgtctt tcgaactgct gaacgcccct gccacagtgt gtggccctaa actgtctaca
     1381 gccctggtca agaaccagtg cgtgaacttc cgggtgcagc ccaccgatag catcgtgcgg
     1441 ttccccaata tcaccaatct gtgccccttc ggcgaggtgt tcaatgccac cacattcgcc
     1501 tctgtgtacg cctggaaccg gaagcggatc agcaattgcg tggccgacta ctccgtgctg
     1561 tacaacagca ccagcttcag caccttcaag tgctacggcg tgtcccctac caagctgaac
     1621 gacctgtgct tcacaaacgt gtacgccgac agcttcgtga tcaccgggga tgaagtgcgg
     1681 cagattgccc ctggacagac aggcaagatc gccgactaca actacaagct gcccgacgac
     1741 ttcaccggct gtgtgattgc ctggaactcc aagcacatcg acgccaaaga aggcgggaac
     1801 ttcaactacc tgtaccggct gttccggaag gccaacctga agcctttcga gcgggatatc
     1861 tccaccgaga tctaccaggc cggcagcaag ccttgtaatg gccagacagg cctgaactgc
     1921 tactacccac tgtacagata cggcttctac cccacagacg gcgtgggcca ccagccttat
     1981 agagtggtgg tgctgagctt cgagctgctg aatgcccctg ccacagtgtg cggccctaag
     2041 aaaagcacca atctcgtgaa gaacaaatgc gtgaacttca acatcaccaa tctgtgcccc
     2101 ttcggcgagg tgttcaatgc cacaagattt gccagcgtgt acgcctggaa ccggaagaga
     2161 atcagcaact gcgtggccga ctacagcgtg ctgtacaata gcgccagctt cagcaccttc
     2221 aagtgctacg gcgtgtcccc taccaagctg aacgacctgt gcttcaccaa tgtgtacgcc
     2281 gacagcttcg tgatcagagg cgacgaagtt cggcagatcg ctcctggaca gacaggcaag
     2341 atcgccgatt acaactacaa gctgcccgac gacttcaccg gctgcgtgat cgcctggaat
     2401 agcaacaacc tggacagcaa agtcggcggc aactacaact acctgtaccg gctgttccgg
     2461 aagtccaacc tgaagccttt cgagcgggac atcagcaccg agatctatca ggccggcagc
     2521 accccttgta atggcgtgga aggcttcaac tgctacttcc cactgcagtc ctacggcttc
     2581 cagcctacaa atggcgtcgg ctaccagcct tacagagtgg tggtgctgtc cttcgaactg
     2641 ctgcatgccc ctgctaccgt gtgcggccct aaaaaaacct ag
//
