LOCUS       sequence-418827-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human CDK6-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(CDK6-E2(15))-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        250..448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     403..423
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             466..646
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    693..818
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1317..1550
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1735..1779
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2077..2194
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2269..2517
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2269..2289
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2440..2459
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2646..3145
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2681..2704)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3078..3097
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3185..3781
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3185..3204)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3681..3701
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3800..3853
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3854..4555
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4615..4635)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5033..5044)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5496..5513)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5502..5726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5752..5772)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5752..5770)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5791..5807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5791..5807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5804..5826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5815..5831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5839..5845,5846..5863,5864..5869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5884..5905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6022..6039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6193..6781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6273..6292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6952..7743,7744..7812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7575..7594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7813..7917)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7943..8398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8080..8101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8292..8311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8525..8547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8539..8556
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8540..8556
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8566..8585
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8566..8584
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
       61 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      121 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      181 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      241 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      301 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      361 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      421 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
      481 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      541 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      601 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      661 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      721 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      781 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      841 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      901 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      961 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1021 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1081 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1141 ccaccgcaca gcaagcggcc ggccgcgctg atcttcagac ctggaggagg agatatgagg
     1201 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1261 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1321 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1381 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1441 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1501 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1561 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1621 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1681 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1741 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1801 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1861 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1921 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1981 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
     2041 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2101 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2161 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2221 tccagtttgg ttagtaccgg gccctacgcg tccaaggtcg ggcaggaaga gggcctattt
     2281 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt
     2341 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     2401 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
     2461 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgaa
     2521 cagtattaat ctgtggtgtt taagagctat gctggaaaca gcatagcaag tttaaataag
     2581 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ggatccaatt
     2641 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     2701 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     2761 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     2821 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     2881 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     2941 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc aggggcgggg
     3001 agggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     3061 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     3121 ctcttcctca tctccgggcc tttcgacctg catccatcta gatctcgagc agctgaagct
     3181 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     3241 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     3301 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     3361 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     3421 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     3481 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     3541 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     3601 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3661 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3721 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     3781 cggcggcggg tccggaggag agggcagagg aagtctccta acatgcggtg acgtggagga
     3841 gaatcctggc ccaatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     3901 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     3961 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     4021 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     4081 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     4141 cgaggacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     4201 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     4261 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     4321 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac
     4381 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     4441 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     4501 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta attgagcggc
     4561 cgctcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4621 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4681 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4741 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4801 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4861 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4921 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4981 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5041 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5101 acgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc aggtaccttt
     5161 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5221 actggaaggg ctaattcact cccaaagaag tcaagatctg ctttttgcct gtactgggtc
     5281 tctctggtta gaccagagtc tctctggtta gaccagatct gagcctggga gctctctggc
     5341 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5401 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5461 tggaaaatct ctagcagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5521 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5581 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5641 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagtca atagcaggca
     5701 tgctggggat gcggtgggct ctatgggcgg ccgttaatta agcttttgtt ccctttagtg
     5761 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5821 tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc
     5881 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5941 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6001 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6061 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6601 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7921 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     7981 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     8041 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     8101 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     8161 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     8221 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     8281 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     8341 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     8401 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8461 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8521 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     8581 tagggcgaat tgactagtac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     8641 gacccccgcc
//
