LOCUS       sequence-418872-        9032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human SMARCA4 and 
            SMARCA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: 
            pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(27..482)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(164..185)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     376..395
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     609..631
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     623..640
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     624..640
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     650..669
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        650..668
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        984..1182
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1137..1157
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1200..1380
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1427..1552
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2051..2284
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2469..2513
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2811..2928
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3385..3633
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3385..3405
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3556..3575
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3762..4261
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3797..3820)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4194..4213
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4301..4897
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4301..4320)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4797..4817
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4916..4969
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4970..5671
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5731..5751)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6149..6160)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6612..6629)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6618..6842
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6868..6888)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6868..6886)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6907..6923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6907..6923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6920..6942)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6931..6947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6955..6961,6962..6979,6980..6985))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7000..7021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7138..7155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7309..7897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7389..7408)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8068..8859,8860..8928))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8691..8710
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8929..9032,1))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
       61 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      121 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      181 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      241 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
      301 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
      361 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
      421 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
      481 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
      541 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      601 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
      661 actatagggc gaattgacta gtacataact tacggtaaat ggcccgcctg gctgaccgcc
      721 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      781 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      841 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      901 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      961 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1021 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1081 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1141 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     1201 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1261 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1321 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1381 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1441 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1501 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1561 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1621 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1681 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1741 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1801 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaacagca
     1861 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc gctgatcttc agacctggag
     1921 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1981 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     2041 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     2101 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     2161 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     2221 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     2281 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     2341 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     2401 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     2461 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     2521 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     2581 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     2641 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     2701 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     2761 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     2821 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2881 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2941 agggacagca gagatccagt ttggttagta ccgggcccta cgcgtctgca gtatttagca
     3001 tgccccaccc atctgcaagg cattctggat agtgtcaaaa cagccggaaa tcaagtccgt
     3061 ttatctcaaa ctttagcatt ttgggaataa atgatatttg ctatgctggt taaattagat
     3121 tttagttaaa tttcctgctg aagctctagt acgataagca acttgaccta agtgtaaagt
     3181 tgagacttcc ttcaggttta tatagcttgt gcgccgcttg ggtacctcga tcagaacgct
     3241 gtccaactgt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     3301 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tagccgaact gtttcacact
     3361 cacgcgtcca aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat
     3421 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     3481 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     3541 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     3601 gctttatata tcttgtggaa aggacgaaac accgttgtcc cccagctacg gaggtttaag
     3661 agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
     3721 agtggcaccg agtcggtgct ttttttggat ccaattctac cgggtagggg aggcgctttt
     3781 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     3841 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     3901 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     3961 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     4021 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     4081 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     4141 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     4201 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     4261 gacctgcatc catctagatc tcgagcagct gaagcttacc atgaccgagt acaagcccac
     4321 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4381 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4441 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4501 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4561 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4621 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4681 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4741 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4801 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4861 accgcgcacc tggtgcatga cccgcaagcc cggtgccggc ggcgggtccg gaggagaggg
     4921 cagaggaagt ctcctaacat gcggtgacgt ggaggagaat cctggcccaa tgagcgagct
     4981 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     5041 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     5101 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     5161 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     5221 ccctgagggc ttcacatggg agagagtcac cacatacgag gacgggggcg tgctgaccgc
     5281 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     5341 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     5401 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     5461 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     5521 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     5581 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     5641 ccctagcaaa ctggggcaca agcttaattg agcggccgct caacctctgg attacaaaat
     5701 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5761 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5821 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5881 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5941 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6001 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6061 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6121 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6181 cggcctgctg ccggctctgc ggcctcttcc gcgtctacgc cttcgccctc agacgagtcg
     6241 gatctccctt tgggccgcct ccccgcaggt acctttaaga ccaatgactt acaaggcagc
     6301 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6361 aagaagtcaa gatctgcttt ttgcctgtac tgggtctctc tggttagacc agagtctctc
     6421 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6481 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6541 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtttaaac
     6601 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6661 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6721 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6781 cagcaagggg gaggattggg aagtcaatag caggcatgct ggggatgcgg tgggctctat
     6841 gggcggccgt taattaagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     6901 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6961 acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7021 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7081 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctgtt ccgcttcctc
     7141 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7201 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7261 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7321 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7381 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7441 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7501 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7561 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7621 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7681 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7741 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7801 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7861 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7921 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7981 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8041 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8101 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8161 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8221 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8281 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8341 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8401 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8461 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8521 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8581 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8641 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8701 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8761 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8821 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8881 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8941 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9001 tatttagaaa aataaacaaa taggggttcc gc
//
