LOCUS       sequence-418875-       10313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Luc2 and tdTomato under EF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10313)
  TITLE     express gene of interest under truncated EF-1 promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Luc2-P2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..10313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..107
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     505..525
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(594..2024)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGIGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             complement(2034..2090)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2106..3755)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     promoter        complement(3771..4949)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3780..4718
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3803..3823)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5047..5163
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5430..5474)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5659..5892
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6385..6510
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6557..6737
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6738..6964)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6979..6996)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6979..6995)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6988..7010)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7204..7223)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7323..7345
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7383..7401)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7469..7573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7574..7642,7643..8434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7792..7811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8605..9193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9347..9364
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9481..9502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9517..9522,9523..9540,9541..9547)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9555..9571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9560..9582
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9579..9595
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9579..9595
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      9628..9763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9803..9924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9814..9833)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9868..9887
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             9996..10229
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    join(10303..10313,1..578)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga
       61 gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag
      121 aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat
      181 gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag
      241 cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc
      301 ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac
      361 acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc
      421 aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac
      481 aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca
      541 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacgaattc ttattacttg
      601 tacagctcgt ccatgccgta caggaacagg tggtggcggc cctcggagcg ctcgtactgt
      661 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc cacgtagtag
      721 tagccgggca gttgcacggg cttcttggcc atgtagatgg tcttgaactc caccaggtag
      781 tggccgccgt ccttcagctt cagggcctgg tggatctcgc ccttcagcac gccgtcgcgg
      841 gggtacaggc gctcggtgga ggcctcccag cccatggtct tcttctgcat tacggggccg
      901 tcggggggga agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc gtcctgcagg
      961 gaggagtcct gggtcacggt caccagaccg ccgtcctcga agttcatcac gcgctcccac
     1021 ttgaagccct cggggaagga cagcttcttg taatcgggga tgtcggcggg gtgcttcacg
     1081 tacgccttgg agccgtacat gaactggggg gacaggatgt cccaggcgaa gggcaggggg
     1141 ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
     1201 tcgccctcga tctcgaactc gtggccgttc atggagccct ccatgcgcac cttgaagcgc
     1261 atgaactctt tgatgacggc catgttgttg tcctcggagg aggcggtgcc ggagctgccg
     1321 ctgccggtgc tgccgatgcc atgccccagg aacaggtggt ggcggccctc ggagcgctcg
     1381 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg
     1441 tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt gaactccacc
     1501 aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt cagcacgccg
     1561 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     1621 gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag cgtgccgtcc
     1681 tgcagggagg agtcctgggt cacggtcacc agaccgccgt cctcgaagtt catcacgcgc
     1741 tcccacttga agccctcggg gaaggacagc ttcttgtaat cggggatgtc ggcggggtgc
     1801 ttcacgtacg ccttggagcc gtacatgaac tggggggaca ggatgtccca ggcgaagggc
     1861 agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
     1921 tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat gcgcaccttg
     1981 aagcgcatga actctttgat gacctcctcg cccttgctca ccattggccc gggaggtcca
     2041 gggttctcct ccacgtctcc agcctgcttc agcaggctga agttagtagc tccgcttcca
     2101 agcttcacgg cgatcttgcc gcccttcttg gccttaatga gaatctcgcg gatcttgcgg
     2161 gcgtccaact tgccggtcag tcctttaggc acctcgtcca cgaacacaac accaccgcgc
     2221 agcttcttgg cggttgtaac ctggctggcc acatagtcca cgatctcctt ctcggtcatg
     2281 gttttaccgt gttccagcac gacgactgcg gcgggcagct cgccggcatc gtcgtcgggc
     2341 aggccggcga ccccggcgtc gaagatgttg gggtgttgca gcaggatgct ctccagttcg
     2401 gctggggcta cctggtagcc cttgtatttg atcaggctct tcagccggtc cacgatgaag
     2461 aagtgctcgt cctcgtccca gtaggcgatg tcgccgctgt gcagccagcc gtccttgtcg
     2521 atgagagcgt ttgtagcctc ggggttgtta acgtagccgc tcatgatcat ggggccacgg
     2581 acgcacagct cgccgcgctg gttcacaccc agtgtcttac cggtgtccaa gtccaccacc
     2641 ttagcctcga agaagggcac caccttgcct actgcgccag gcttgtcgtc cccttcgggg
     2701 gtgatcagaa tggcgctggt tgtttctgtc aggccgtagc cctggcggat gcctggtagg
     2761 tggaagcgtt tggccacggc ctcacctacc tccttgctga gcggcgcccc gccgctggcg
     2821 atctcgtgca agttgcttag gtcgtacttg tcgatgagag tgctcttagc gaagaagcta
     2881 aatagtgtgg gcaccagcag ggcagattga atcttatagt cttgcaagct gcgcaagaat
     2941 agctcctcct cgaagcggta catgagcacg acccgaaagc cgcagatcaa gtagcccagc
     3001 gtggtgaaca tgccgaagcc gtggtgaaat ggcaccacgc tgaggatagc ggtgtcgggg
     3061 atgatctggt tgccgaagat ggggtcgcgg gcatgactga atcggacaca agcggtgcgg
     3121 tgcggtaggg ctacgccctt gggcaatccg gtactgccac tactgttcat gatcagggcg
     3181 atggttttgt cccggtcgaa gctctcgggc acgaagtcgt actcgttgaa gccgggtggc
     3241 aaatgggaag tcacgaaggt gtacatgctt tggaagccct ggtagtcggt cttgctatcc
     3301 atgatgatga tcttttgtat gatcggtagc ttcttttgca cgttgaggat cttttgcagc
     3361 cctttcttgc tcacgaatac gacggtgggc tggctgatgc ccatgctgtt cagcagctcg
     3421 cgctcgttgt agatgtcgtt agctggggcc acagccacac cgatgaacag ggcacccaac
     3481 acgggcatga agaactgcaa gctattctcg ctgcacacca cgatccgatg gtttgtattc
     3541 agcccatagc gcttcatagc ttctgccagc cgaacgctca tctcgaagta ctcggcgtag
     3601 gtaatgtcca cctcgatatg tgcgtcggta aaggcgatgg tgccgggcac cagggcgtag
     3661 cgcttcatgg ctttgtgcag ctgctcgccg gcggtcccgt cttcgagtgg gtagaatggc
     3721 gctgggccct tcttaatgtt tttggcatct tccatggtgg atccaattcc tcacgacacc
     3781 tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca
     3841 aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca
     3901 gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa
     3961 gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc
     4021 gtggagtcac atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac
     4081 tcacccgccc gctctcccga gcgccgcgtc ctccattttg agctccctgc agcagggccg
     4141 ggaagcggcc atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag
     4201 ggcggggcga tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac
     4261 tacccccgtc cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc
     4321 tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga
     4381 tcttggcccg catttacaag actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa
     4441 aaattttaaa tggctagaga cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag
     4501 attcgcacgc ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg
     4561 gctccttaag cgcaaggcct cgaactctcc cacccacttc caacccgaag ctcgggatca
     4621 agaatcacgt actgcagcca ggtggaagta attcaaggca cgcaagggcc ataacccgta
     4681 aagaggccag gcccgcggga accacacacg gcacttacct gtgttctggc ggcaaacccg
     4741 ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg
     4801 ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc
     4861 taggcaccgg ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg
     4921 cgctctgccc actgacgggc accggagcca attcccactc ctttcaagac ctagaaggtc
     4981 cattagctgc aaagattcct ctctgtttaa aactttatcc atctttgcaa agctatcgat
     5041 atcgataaaa ttttgaattt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     5101 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     5161 aaagttaacc gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc
     5221 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg
     5281 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
     5341 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
     5401 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
     5461 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
     5521 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc
     5581 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca
     5641 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt
     5701 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca
     5761 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt
     5821 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg
     5881 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg
     5941 gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc
     6001 caattgtccc tcatatctcc tcctccaggt ctgaagatca gtggccgctt gctgtgcggt
     6061 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     6121 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     6181 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     6241 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     6301 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     6361 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     6421 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     6481 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     6541 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     6601 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     6661 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     6721 tctaaccaga gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa
     6781 tgcggcaatt cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag
     6841 gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt
     6901 aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagagtat tgcataagac
     6961 tacacgcgtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7021 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7081 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     7141 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     7201 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     7261 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7321 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     7381 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7441 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7501 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7561 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7621 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7681 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7741 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7801 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7861 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7921 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7981 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8041 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8101 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8161 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8221 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8281 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8341 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8401 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8461 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8521 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8581 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8641 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8701 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8761 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8821 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8881 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8941 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9001 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9061 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9121 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9181 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9241 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9301 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9361 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9421 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9481 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9541 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     9601 tacgaatttg gtctctgcaa aagtctaggc ctccaaaaaa gcctcctcac tacttctgga
     9661 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     9721 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     9781 ggatagctag agccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     9841 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     9901 attataagct gcaataaaca agttcctctc actctctgat attcatttct ttgcaagtta
     9961 taaatactga ataataagat gacatgaact actactgcta gagattttcc acactgacta
    10021 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
    10081 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
    10141 ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatctta
    10201 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
    10261 atctacagct gccttgtaag tcattggtct taaaggtacc aggcggggag gcg
//
