LOCUS       sequence-418876-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human RHOA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(RHOA(12))-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        262..460
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     415..435
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             478..658
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    705..830
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1329..1562
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1747..1791
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2089..2206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2281..2529
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2281..2301
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2452..2471
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2658..3157
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2693..2716)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3090..3109
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3197..3793
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3197..3216)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3693..3713
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3812..3865
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3866..4567
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4627..4647)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5045..5056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5508..5525)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5514..5738
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5764..5784)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5764..5782)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5803..5819)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5803..5819)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5816..5838)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5827..5843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5851..5857,5858..5875,5876..5881))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5896..5917
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6034..6051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6205..6793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6285..6304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6964..7755,7756..7824))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7587..7606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7825..7929)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7955..8410)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8092..8113)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8304..8323
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8537..8559
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8551..8568
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8552..8568
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8578..8597
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8578..8596
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
       61 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      121 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      181 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      241 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      301 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      361 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      421 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      481 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      541 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      601 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      661 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      721 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      781 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      841 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      901 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      961 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1021 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1081 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1141 acaaaagtaa gaccaccgca cagcaagcgg ccggccgcgc tgatcttcag acctggagga
     1201 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1261 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1321 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1381 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1441 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1501 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1561 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1621 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1681 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1741 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1801 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1861 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1921 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     1981 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2041 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2101 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2161 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2221 ggacagcaga gatccagttt ggttagtacc gggccctacg cgtccaaggt cgggcaggaa
     2281 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     2341 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     2401 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     2461 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2521 cgaaacaccg cacatacacc tctgggaacg tttaagagct atgctggaaa cagcatagca
     2581 agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     2641 ttggatccaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     2701 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     2761 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     2821 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     2881 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     2941 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     3001 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     3061 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     3121 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga
     3181 gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     3241 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     3301 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     3361 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     3421 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     3481 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     3541 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     3601 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     3661 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     3721 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     3781 caagcccggt gccggcggcg ggtccggagg agagggcaga ggaagtctcc taacatgcgg
     3841 tgacgtggag gagaatcctg gcccaatgag cgagctgatt aaggagaaca tgcacatgaa
     3901 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg
     3961 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
     4021 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca
     4081 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag
     4141 agtcaccaca tacgaggacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     4201 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
     4261 gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg
     4321 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc
     4381 aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt
     4441 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga
     4501 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct
     4561 taattgagcg gccgctcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4621 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4681 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4741 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4801 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4861 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4921 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4981 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5041 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5101 tcttccgcgt ctacgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5161 gcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5221 agaaaagggg ggactggaag ggctaattca ctcccaaaga agtcaagatc tgctttttgc
     5281 ctgtactggg tctctctggt tagaccagag tctctctggt tagaccagat ctgagcctgg
     5341 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5401 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5461 ttttagtcag tgtggaaaat ctctagcagt ttaaacccgc tgatcagcct cgactgtgcc
     5521 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5581 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5641 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaagt
     5701 caatagcagg catgctgggg atgcggtggg ctctatgggc ggccgttaat taagcttttg
     5761 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     5821 gtgaaattgt tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa
     5881 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5941 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6001 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6061 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6121 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6181 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6241 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6301 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6361 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6421 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6481 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6541 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6601 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6661 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6721 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6781 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6901 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6961 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7021 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7081 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7141 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7201 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7261 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7321 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7381 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7441 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7501 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7561 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7621 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7681 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7741 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7801 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7861 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7921 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     7981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     8221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     8281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     8341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     8401 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     8461 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     8521 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     8581 tacgactcac tatagggcga attgactagt acataactta cggtaaatgg cccgcctggc
     8641 tgaccgccca
//
